[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (7): 651-.doi: 10.3736/jcim20090709

• Original Experimental Research • Previous Articles     Next Articles

Dachengqi Decoction induces pancreatic acinar cell apoptosis in experimental acute pancreatitis in rats

Yan-yi Ren, Han-lin Gong, Wen-Fu Tang,Mei-hua Wan, Jian-lei Zhao, Xi Huang   

  1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2009-03-24 Accepted:2009-04-30 Online:2009-07-20 Published:2009-07-15
  • Contact: Wen-Fu Tang E-mail:wftang900@126.com

RichHTML

3

PDF (PC)

84

Like

Objective

To explore the effects of Dachengqi Decoction (DCQD), a compound traditional Chinese herbal medicine, on pancreatic acinar cell apoptosis in a rat model of experimental acute pancreatitis.

Methods

A total of 36 Sprague-Dawley rats were randomly divided into sham-operated group, untreated group and DCQD-treated group (12 rats in each group). Acute pancreatitis was induced in 24 rats by retrograde injection of 3.5% sodium taurocholate into pancreatic bile duct. The other 12 rats were allocated as sham-operated group. After the operation, the spray-dried DCQD (2 g/mL of crude drugs) or normal saline at 10 mL/kg body weight of rats were orally administered. The rats were sacrificed by decapitation 12 h and 24 h after the administration, and samples were collected. Amylase activity in serum, nitric oxide (NO) content and inducible NO synthetase (iNOS) activity in pancreatic tissue were measured respectively. Pancreatic acinar cell apoptosis was identified by terminal deoxy-nucleotidyl transferase mediated dUTP nick end-labeling, and pathological scores of pancreatic tissues were determined under a light microscope.
Results

At the two time points after treatment, the activities of serum amylase in the treated group were significantly lower than those in the untreated group (P<0.05), while the contents of pancreatic NO and activities of iNOS were higher than those in the untreated group (P<0.05), respectively. The pancreatic acinar cell apoptosis rates in the treatment group at 12 h and 24 h were higher than those in the untreated group, and the mean pancreatic pathomorphologic scores decreased correspondingly (P<0.05).
Conclusion

DCQD can induce pancreatic acinar cell apoptosis by increasing NO content and iNOS activity in the pancreas of experimental acute pancreatitis, which helps attenuate the pancreatic pathomorphology.

Key words: Dachengqi Decoction, Acute pancreatitis, Apoptosis, Pathomorphology, Rats

Table 1

Results of therapeutic indications in different groups ($\bar{x}$±s)"

Time Group n Amylase (U/L) NO (μmol/L) iNOS (U/mg) Apoptosis index (%) Pathology score
12 h Sham-operated 6 1 282±107 31.3±1.5 0.58±0.02 0.72±0.11 0.31±0.02
Untreated 6 7 418±559 6.8±0.5 0.38±0.02 5.40±1.24 3.95±0.25
Treated 6 4 996±422* 50.1±4.7* 0.49±0.03* 11.90±0.72* 2.98±0.28*
24 h Sham-operated 6 1 141±113 31.3±1.4 0.57±0.02 0.87±0.14 0.43±0.09
Untreated 6 6 952±545 8.5±0.9 0.36±0.02 3.54±0.65 4.67±0.31
Treated 6 4 238±379*△△ 62.2±2.2*△△ 0.43±0.06*△△ 21.50±4.30*△△ 3.15±0.25*△△

Figure 1

Pathological changes of pancreas in different groups observed by HE staining (Light microscopy, ×400) A: Sham-operated group; B: Untreated group; C: Treated group."

[1] Xia Q, Huang ZW, Jiang JM, Chen GY, Yang XN, Tang WF, Zhang ZD, Liu XB, Hu WM, Tian BL, Li QS, Yan LN, Luo CX, Kang Y, Yuan CX, Song B, Luo Y . "Yi-Huo-Qing-Xia" method as the main therapy in integrated traditional Chinese and Western medicine on severe acute pancreatitis: a report of 1 161 cases[J]. Zhongguo Zhong Xi Yi Jie He Ji Jiu Za Zhi, 2006,13(3):131-134
夏庆, 黄宗文, 蒋俊明, 陈光远, 杨晓楠, 唐文富, 张肇达, 刘续宝, 胡伟明, 田伯乐, 李全生, 严律南, 罗传兴, 康焰, 袁朝新, 宋彬, 罗燕 . 以"益活清下"为主的中西医结合综合疗法治疗重症急性胰腺炎1 161例疗效报告[J]. 中国中西医结合急救杂志,
[2] Xia Q, Jiang JM, Gong X, Chen GY, Li L, Huang ZW . Experimental study of Tong Xia purgative method in ameliorating lung injury in acute necrotizing pancreatitis[J]. World J Gastroenterol, 2000,6(1):115-118
doi: 10.3748/wjg.v6.i1.115
[3] Tang WF, Wan MH, Zhu L, Chen GY, Xia Q, Huang X . Immuno-modulatory effect of somatostatin combined with traditional Chinese medicine on severe acute pancreatitis at early stage: a randomized control trial[J]. J Chin Integr Med, 2005,3(2):103-107
唐文富, 万美华, 朱林, 陈光远, 夏庆, 黄熙 . 生长抑素联合中药对急性胰腺炎患者免疫功能的影响及临床疗效[J]. 中西医结合学报, 2005,3(2):103-107
[4] Tang W, Wan M, Zhu Z, Chen G, Huang X . Simultaneous determination of eight major bioactive compounds in Dachengqi Tang(DT) by high-performance liquid chromatography[J]. Chin Med, 2008,( 3):5
[5] Hietaranta AJ, Peuravuori HJ, Nevalainen TJ . Phospholipase A2 in sodium taurocholate-induced experimental hemorrhagic pancreatitis in the rat[J]. J Surg Res, 1995,59(2):271-278
doi: 10.1006/jsre.1995.1164 pmid: 7637343
[6] Weber H, Wagner ACC, Jonas L, Merkord J, Hofken T, Nizze H, Leitzmann P, Goke B, Schuff-Werner P . Heat shock response is associated with protection against acute interstitial pancreatitis in rats[J]. Dig Dis Sci, 2000,45(11):2252-2264
doi: 10.1023/A:1026459001195
[7] Rakonczay Z Jr, Takács T, Ivànyi B, Màndi Y, Pàpai G, Boros I, Varga IS, Jost K, Lonovics J . The effects of hypo- and hyperthermic pretreatment on sodium taurocholate-induced acute pancreatitis in rats[J]. Pancreas, 2002,24(1):83-89
doi: 10.1097/00006676-200201000-00011
[8] Kaiser AM, Saluja AK, Sengupta A, Saluja M, Steer ML . Relation between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis[J]. Am J Physiol, 1995,269(5 Pt 1):C1295-C1304
doi: 10.1152/ajpcell.1995.269.5.C1295
[9] Saluja A, Hofbauer B, Yamaguchi Y, Yamanaka K, Steer M . Induction of apoptosis reduces the severity of caerulein-induced pancreatitis in mice[J]. Biochem Biophys Res Commun, 1996,220(3):875-878
doi: 10.1006/bbrc.1996.0498
[10] Kaiser AM, Saluja AK, Lu L, Yamanaka K, Yamaguchi Y, Steer ML . Effects of cycloheximide on pancreatic endonuclease activity, apoptosis, and severity of acute pancreatitis[J]. Am J Physiol, 1996,271(3 Pt 1):C982-C993
doi: 10.1152/ajpcell.1996.271.3.C982
[11] Flint RS, Phillips AR, Power SE, Dunbar PR, Brown C, Delahunt B, Cooper GJ, Windsor JA . Acute pancreatitis severity is exacerbated by intestinal ischemia-reperfusion conditioned mesenteric lymph[J]. Surgery, 2008,143(3):404-413
doi: 10.1016/j.surg.2007.10.005
[12] Wacke R, Park S, Mundkowski RG, Block N, Kuhn-Thiel A, Drewelow B . The penetration of moxifloxacin into the pancreas of male rats in experimental acute necrotizing pancreatitis[J]. Chemotherapy, 2003,49(4):167-171
doi: 10.1159/000071140
[13] Mizunuma H, Khorram O , McCann SM.Blockade of stress-induced prolactin release in monosodium glutamate-treated rats[J]. Brain Res Bull, 1983,10(1):23-26
doi: 10.1016/0361-9230(83)90068-0 pmid: 6297690
[14] Ramkumar D, Schulze KS . Gastroduodenal motility[J]. Curr Opin Gastroenterol, 2003,19(6):540-545
doi: 10.1097/MOG.0b013e32833ece1e pmid: 15703602
[15] Yang J, Fier A, Carter Y, Liu G, Epling-Burnette PK, Bai F, Loughran TP Jr, Mastorides S, Norman JG, Murr MM . Liver injury during acute pancreatitis: the role of pancreatitis-associated ascitic fluid(PAAF), p38-MAPK, and caspase-3 in inducing hepatocyte apoptosis[J]. J Gastrointest Surg, 2003,7(2):200-208
doi: 10.1016/S1091-255X(02)00134-8
[16] Hahm KB, Kim JH, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB . Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats[J]. Pancreas, 1998,17(2):153-157
doi: 10.1097/00006676-199808000-00007
[17] Um SH, Kwon YD, Kim CD, Lee HS, Jeen YT, Chun HJ, Lee SW, Choi JH, Ryu HS, Hyun JH . The role of nitric oxide in experimental cerulein induced pancreatitis[J]. J Korean Med Sci, 2003,18(4):520-526
doi: 10.3346/jkms.2003.18.4.520 pmid: 12923328
[18] Werner J , Fernández-del Castillo C, Rivera JA, Kollias N, Lewandrowski KB, Rattner DW, Warshaw AL.On the protective mechanisms of nitric oxide in acute pancreatitis[J]. Gut, 1998,43(3):401-407
doi: 10.1136/gut.43.3.401 pmid: 9863487
[19] Zhang ZX, Sun JB, Li F, Zhang SW, Cui YQ, Sun HC, Liu S . The protective effect of endogenous nitric oxide on acute necrotic pancreatitis in rats[J]. Zhonghua Pu Tong Wai Ke Za Zhi, 2000,15(10):606-608
doi: 10.3760/j.issn:1007-631X.2000.10.010
张在兴, 孙家邦, 李非, 张淑文, 崔叶青, 孙海晨, 刘爽 . 内源性一氧化氮对大鼠急性坏死性胰腺炎的胰腺保护作用[J]. 中华普通外科杂志, 2000,15(10):606-608
doi: 10.3760/j.issn:1007-631X.2000.10.010
[20] Wu T, Chen X, Ji ZZ . Effect of L-arginine on pancreatic acinar cell apoptosis in rats with acute pancreatitis[J]. Di Si Jun Yi Da Xue Xue Bao, 2007,28(17):1570-1572
吴涛, 陈熹, 纪宗正 . 精氨酸对急性胰腺炎大鼠胰腺腺泡细胞凋亡的影响[J]. 第四军医大学学报, 2007,28(17):1570-1572
[21] Thatte U, Bagadey S, Dahanukar S . Modulation of programmed cell death by medicinal plants[J]. Cell Mol Biol (Noisy-le-grand), 2000,46(1):199-214
doi: 10.1247/csf.25.57 pmid: 10726985
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
[3] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[4] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[5] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[6] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[7] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[8] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[9] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[10] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[13] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[14] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[15] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]