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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (7): 661-666.doi: 10.3736/jcim20090711

• Original Experimental Research • Previous Articles     Next Articles

Mechanism of combined treatment with Shenkangling Decoction and prednisone in rats with adriamycin-induced nephropathy

Qing Lina,Jian Zhengb,Si Aib,Li-xing Lina,Yu-xin Chena,Fang Wanga,Ling Zhuc   

  1. a Clinical Laboratory, People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian Prov¬ince, China;
    b Department of Pediatrics, People5 s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian Province, China
    c Department of Immunology, Fujian Medical University, Fuzhou 350004, Fujian Province, China
  • Received:2009-03-19 Accepted:2009-05-05 Online:2009-07-20 Published:2009-07-15
  • Contact: Qing Lin E-mail:fjlinqing@126.com

Objective

To study the mechanism of Shenkangling (SKL), a compound traditional Chinese herbal medicine, combined with prednisone in treating adriamycin-induced nephropathy in rats.

Methods

Sixty-six SD male rats were randomly divided into normal control group, untreated group, prednisone group, SKL group and SKL plus prednisone group. Except the normal control group, rats were injected once via caudal vein with adriamycin (5.5 mg/kg) to induce nephropathy. Then, the rats were administered with prednisone, SKL, prednisone plus SKL or distilled water for 3 weeks, respectively. After harvest, 24-hour urine protein excretion, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) contents in serum, and content of nuclear transcription factor-kappa B (NF-κB) p65 in mononuclear cells were determined, and correlation analysis among these parameters was performed. The content of NF-κB p65 was assayed with the patented method of Active Motif; TNF-α was assayed with enzyme-linked immunosorbent assay, and the content of NO was assessed by the method of nitrate reductase. The change of foot process in renal glomerulus was observed under an electron microscope.
Results

When the rats were administered with prednisone, SKL correspondingly, the contents of NF-κB p65, TNF-α and NO as well as 24-hour urine protein excretion were lower than those in the untreated group (P<0.01), and the fusion of foot process only recovered partially. Compared with other treated groups, the contents of NF-κB p65 and NO as well as 24-hour urine protein excretion were significantly decreased in SKL plus prednisone group (P<0.01, P<0.05), and the fusion of foot process recovered mostly also. There was no interaction between SKL and prednisone in decreasing the content of TNF-α.
Conclusion

Renal injury can be postponed and improved when treated with SKL puls prednisone; it may contribute to the depression of abnormal activation of NF-κB, and the inhibition of production of TNF-α and NO.

Key words: Adriamycin-induced nephropathy, Shenkangling, prednisone, NF-kappa B p65 subunit, Tumor necrosis factor-a, Nitric oxide, Rats

Table 1

Twenty-four hour urine protein excretion at different treatment time in different groups ($\bar{x}$±s, mg)"

Prednisone SKL 24-hour urine protein
n Week 1 n Week 2 n Week 3
No No 10 189.08±38.15 9 287.06±40.68 8 327.29±37.17
Yes No 10 173.98±32.30 10 203.38±37.76 10 197.91±35.34
No Yes 10 186.05±30.02 10 250.52±42.65 10 281.15±41.14
Yes Yes 10 140.07±23.40 10 153.22±25.04 10 157.58±29.61

Table 2

TNF-α content in serum at different treatment time in different groups ($\bar{x}$±s, ng/L)"

Prednisone SKL TNF-α
n Week 1 n Week 2 n Week 3
No No 10 15.01±3.91 9 20.50±3.81 8 23.91±3.46
Yes No 10 12.63±2.27 10 12.06±2.48 10 11.57±2.80
No Yes 10 13.89±2.64 10 17.22±3.63 10 17.62±3.28
Yes Yes 10 11.92±2.06 10 10.09±1.74 10 7.41±1.86

Table 3

NO content in serum at different treatment time in different groups ($\bar{x}$±s, μmol/L)"

Prednisone SKL NO
n Week 1 n Week 2 n Week 3
No No 10 81.10±12.46 9 97.81±10.71 8 114.80 10.66
Yes No 10 75.14±11.33 10 84.10±8.80 10 59.90±10.86
No Yes 10 81.43±9.78 10 91.09±7.07 10 85.21±8.79
Yes Yes 10 72.60±11.05 10 62.34±11.97 10 50.07±8.66

Figure 1

Foot process of renal glomerulus in different groups observed by transmission electron microscopy (×9 000) A: Normal control group; B: Untreated group; C: SKL plus prednisone group; D: Prednisone group; E: SKL group. The arrows point to the foot process of renal glomerulus."

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