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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1052-1056.doi: 10.3736/jcim20091106

• Original Clinical Research • Previous Articles     Next Articles

Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis

Zai-lai Jianga,Wei Zhangb,Hui Zhanga,Yi-bo Liub,Qing-ya Lia,Shi-bing Sua   

  1. a Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Department of Hepatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-07-20 Accepted:2009-09-23 Online:2009-11-20 Published:2009-11-15
  • Contact: Shi-bing Su E-mail:shibingsu@yahoo.com

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Objective

To investigate the relationship between single-nucleotide polymorphisms (SNPs) of interleukin-10 (IL-10) and syndrome types of traditional Chinese medicine (TCM) in posthepatitis B cirrhosis.
Methods

The genotypes of IL-10-592 A/C, -819 C/T and -1082 G/A sites were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and polymerase chain reaction-ligase detection reaction (PCR-LDR) combined with the sequencing analysis in 226 cases of posthepatitis B cirrhosis. The genotype and allele frequency distribution, and the relationship between the SNPs and TCM syndromes were analyzed.
Results

The frequency of allele C at IL-10-819 point in spleen deficiency with overabundance of dampness syndrome was significantly higher than that in non-spleen deficiency with overabundance of dampness (P < 0.01) syndrome, and genotype TT in liver stagnation syndrome was significantly higher than that in non-liver stagnation syndrome (P < 0.05). There was no significant relationship between the polymorphisms of IL-10-592 A/C, -1082 G/A and the TCM syndromes in posthepatitis B cirrhosis (P>0.05).
Conclusion

In patients with posthepatitis B cirrhosis, allele C in IL-10-819 locus may be related to spleen deficiency with overabundance of dampness syndrome, and TT genotype in IL-10-819 locus may be related to liver stagnation syndrome.

Key words: Hepatitis B, Liver cirrhosis, Polymorphism, Single nucleotide, Syndrome

Table 1

Sequences of primers, probes and size of products in PCR-LDR"

Site Primers Probes Product
IL-10-1082G/A S1-F: CTG GCT CCC
CTT ACC TTC TAC
S1-R: TCC TTT ACC
CCG ATT TCA TTA		 S1-TA: tttt AAC ACT ACT AAG GCT TCT TTC GGAA
S1-TG: ttttttt AAC ACT ACT AAG GCT TCT TTC GGAG
S1-TR: -P-GGG GAA GTA GGG ATA GGT AAG AGG Attt-FAM		 57 bp/A
60 bp/G
IL-10-819C/T S1-F：CTG GCT CCC
CTT ACC TTC TAC
S1-R：TCC TTT ACC
CCG ATT TCA TTA		 S2-TC: TGT ACC CTT GTA CAG GTG AAG TAAC
S2-TT: ttt TGT ACC CTT GTA CAG GTG AAG TAAT
S2-TR: -P-ATC TCT GTG CCT CAG TTT GCT CAC T-FAM		 50 bp/C
53 bp/T

Table 2

Genotypes of IL-10-592 A/C and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis"

Syndrome type Genotype P
Liver stagnation 20 16 6 22 0.404
Spleen deficiency with overabundance of dampness 17 18 3 21 0.641
Damp-heat accumulation 14 20 5 25 0.571
Liver-kidney yin deficiency 17 23 6 29 0.636
Blood stagnation 20 25 7 32 0.766
Spleen-kidney yang deficiency 4 5 0 5 1.000
Total 92 107 27 134

Table 3

Genotypes of IL-10-819 C/T and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis"

Syndrome type Genotype P
CC CT TT CT+CC
Liver stagnation 3 17 22 20 0.043
Spleen deficiency with overabundance of dampness 18 18 2 36 0.000
Damp-heat accumulation 4 20 15 24 0.753
Liver-kidney yin deficiency 3 25 18 28 0.673
Blood stagnation 8 25 19 33 0.925
Spleen-kidney yang deficiency 0 4 5 4 0.294
Total 36 109 81 145

Table 4

Genotypes of IL-10-1082A/G and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis"

Syndrome type Genotype P
CC CT TT CT+CC
Liver stagnation 38 4 0 4 0.797
Spleen deficiency with overabundance of dampness 33 5 0 5 0.832
Damp-heat accumulation 34 5 0 5 0.877
Liver-kidney yin deficiency 39 7 0 7 0.541
Blood stagnation 48 4 0 4 0.471
Spleen-kidney yang deficiency 7 2 0 2 0.307
Total 199 27 0 27
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