乙型肝炎后肝硬化中医证候与白细胞介素10基因多态性的关联

doi:10.3736/jcim20091106

Abstract

Objective

To investigate the relationship between single-nucleotide polymorphisms (SNPs) of interleukin-10 (IL-10) and syndrome types of traditional Chinese medicine (TCM) in posthepatitis B cirrhosis.
Methods

The genotypes of IL-10-592 A/C, -819 C/T and -1082 G/A sites were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and polymerase chain reaction-ligase detection reaction (PCR-LDR) combined with the sequencing analysis in 226 cases of posthepatitis B cirrhosis. The genotype and allele frequency distribution, and the relationship between the SNPs and TCM syndromes were analyzed.
Results

The frequency of allele C at IL-10-819 point in spleen deficiency with overabundance of dampness syndrome was significantly higher than that in non-spleen deficiency with overabundance of dampness (P < 0.01) syndrome, and genotype TT in liver stagnation syndrome was significantly higher than that in non-liver stagnation syndrome (P < 0.05). There was no significant relationship between the polymorphisms of IL-10-592 A/C, -1082 G/A and the TCM syndromes in posthepatitis B cirrhosis (P>0.05).
Conclusion

In patients with posthepatitis B cirrhosis, allele C in IL-10-819 locus may be related to spleen deficiency with overabundance of dampness syndrome, and TT genotype in IL-10-819 locus may be related to liver stagnation syndrome.

Key words: Hepatitis B, Liver cirrhosis, Polymorphism, Single nucleotide, Syndrome

Zai-lai Jiang, Wei Zhang, Hui Zhang, Yi-bo Liu, Qing-ya Li, Shi-bing Su. Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis. Journal of Chinese Integrative Medicine, 2009, 7(11): 1052-1056.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20091106

http://www.jcimjournal.com/EN/Y2009/V7/I11/1052

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References 12

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Site	Primers	Probes	Product
IL-10-1082G/A	S1-F: CTG GCT CCC CTT ACC TTC TAC S1-R: TCC TTT ACC CCG ATT TCA TTA	S1-TA: tttt AAC ACT ACT AAG GCT TCT TTC GGAA S1-TG: ttttttt AAC ACT ACT AAG GCT TCT TTC GGAG S1-TR: -P-GGG GAA GTA GGG ATA GGT AAG AGG Attt-FAM	57 bp/A 60 bp/G
IL-10-819C/T	S1-F：CTG GCT CCC CTT ACC TTC TAC S1-R：TCC TTT ACC CCG ATT TCA TTA	S2-TC: TGT ACC CTT GTA CAG GTG AAG TAAC S2-TT: ttt TGT ACC CTT GTA CAG GTG AAG TAAT S2-TR: -P-ATC TCT GTG CCT CAG TTT GCT CAC T-FAM	50 bp/C 53 bp/T

Syndrome type	Genotype				P
	CC	CT	TT	CT+CC
Liver stagnation	3	17	22	20	0.043
Spleen deficiency with overabundance of dampness	18	18	2	36	0.000
Damp-heat accumulation	4	20	15	24	0.753
Liver-kidney yin deficiency	3	25	18	28	0.673
Blood stagnation	8	25	19	33	0.925
Spleen-kidney yang deficiency	0	4	5	4	0.294
Total	36	109	81	145

Syndrome type	Genotype				P
	CC	CT	TT	CT+CC
Liver stagnation	38	4	0	4	0.797
Spleen deficiency with overabundance of dampness	33	5	0	5	0.832
Damp-heat accumulation	34	5	0	5	0.877
Liver-kidney yin deficiency	39	7	0	7	0.541
Blood stagnation	48	4	0	4	0.471
Spleen-kidney yang deficiency	7	2	0	2	0.307
Total	199	27	0	27

Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis

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