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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (2): 168-172.doi: 10.3736/jcim20100212

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yiniao Recipe on serum antidiuretic hormone level and plasma ratio of cAMP to cGMP in rats with kidney-yang deficiency

 Wen Sua, Ai-xia Hua, Heng Dua, Kai Yuanb, Hui-fu Xua   

  1. a Department of Pediatrics, Wuhan Hospital of Integrated Chinese and Western Medicine, Wuhan 430022, Hubei Province, China
    b Department of Integrated Chinese and Western medicine, Children Hospital of Wuhan, Wuhan 430020, Hubei Province, China
  • Received:2009-11-12 Accepted:2009-12-25 Online:2010-02-20 Published:2010-02-15
  • Contact: Wen Su E-mail:shuwen_008@126.com

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Objective

To investigate the effects of Yiniao Recipe, a compound traditional Chinese herbal medicine, on contents of serum antidiuretic hormone, and plasma cyclic adenosine monophosphate and cyclic guanosine monophosphate in rats with kidney-yang deficienc
Methods

Forty male Wistar rats were randomly divided into blank control group, untreated group, desmopressin (Minirin) group, low-dose Yiniao Recipe group and high-dose Yiniao Recipe group, with 8 rats in each group. Rats in the blank control group were injected with 0.2 mL normal saline, and rats in the other groups were given intramuscular injection of hydrocortisone 25 mg/kg, 1 time daily for 21 consecutive days; from the 8th day of injection, rats were given double distilled water, Minirin, and high- and low-dose Yiniao Recipe respectively for 30 days. Before and after treatment, 24-hour urine volume was observed, and serum antidiuretic hormone (AVP) as well as plasma cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) contents were detected by enzyme-linked immunosorbent assay. The cAMP/cGMP ratio and morphological changes in renal tissues were also observed.
Results

Compared with blank control group, 24-hour urine volume, serum AVP content and cAMP/cGMP ratio in the untreated group were decreased; compared with the untreated group, Minirin and Yiniao Recipe at low and high doses reduced 24-hour urine volume and increased serum AVP content and cAMP/cGMP ratio significantly (P<0.05 or P<0.01). There were no obvious pathological changes in renal tissue in all groups.
Conclusion

Yiniao Recipe may reduce 24-hour urine volume by increasing serum AVP content and regulating the ratio of cAMP to cGMP in kidney-yang deficiency rats.

Key words: Yinao Recipe, Kidney-yang deficiency, Enuresis, Antidiuretic hormone, Rats

Table 1

Volume of 24-hour urine of rats in different groups ($\bar{x}±s$, mL)"

Group 24-hour urine volume
n Before model-making After model establishment After treatment
Blank control 8 34.51±5.83 39.13±6.28 35.35±6.32
Untreated 8 35.21±5.39 76.31±17.59**▲▲ 82.67±16.02△▲▲
Minirin 8 33.13±5.78 71.30±8.82**▲▲ 54.67±13.51△△□□
Low-dose Yinao Recipe 8 35.43±5.12 74.29±8.03**▲▲ 54.93±14.13△△□□
High-dose Yinao Recipe 8 33.68±8.63 71.59±6.59**▲▲ 50.45±12.79△△□□

Table 2

Contents of serum AVP and plasma cAMP and cGMP, and ratio of cAMP/cGMP in rats in different groups ($\bar{x}±s$)"

Group n AVP (μg/L) cAMP (nmol/L) cGMP (nmol/L) cAMP/cGMP
Blank control 8 8.53±1.37 199.24±24.47 11.17±3.17 18.89±4.77
Untreated 8 4.51±0.89▲▲ 143.81±37.39▲▲ 21.76±3.57▲▲ 6.68±1.59▲▲
Minirin 8 7.74±1.24□□ 182.15±30.97□□ 13.42±4.76□□ 14.81±4.51□□
Low-dose Yinao Recipe 8 6.29±1.06▲▲□□■ 164.96±29.61□□ 18.13±4.83□□ 9.64±2.88▲▲■
High-dose Yinao Recipe 8 7.96±1.12□□☆☆ 186.07±38.63□□ 13.02±2.87□□ 15.01±5.15□□☆☆

Figure 1

Renal pathology in rats observed by light microscopy (HE staining, ×400)A: Blank control group; B: Untreated group; C: Minirin Group; D: Low-dose Yinao Recipe group; E: High-dose Yinao Recipe group."

[1] Wan LS . Etology of children enuresis[J]. Hunan Zhong Yi Yao Dao Bao, 2003,9(1):18-19, 26
万力生 . 小儿遗尿症病因的再认识[J]. 湖南中医药导报, 2003,9(1):18-19, 26
[2] Yang JY . Clinical diagnosis and treatment of infantile enuresis and research status quo[J]. Zhonghua Er Ke Za Zhi, 2000,38(5):335-336
杨霁云 . 小儿遗尿症临床诊治及研究的现状——第二届国际小儿排尿节制学会会议侧记[J]. 中华儿科杂志, 2000,38(5):335-336
[3] Kong XM . Acupuncture treatment for 72 cases of enuresis in children[J]. Jilin Zhong Yi Yao, 2005,25(8):31-32
孔杏梅 . 针灸治疗小儿遗尿72例[J]. 吉林中医药, 2005,25(8):31-32
[4] Zhang WR . Experimental study in animal model with kidney-yang deficiency and its outlook[J]. Zhong Yi Yao Xue Kan, 2006,24(10):1809-1810
张伟荣 . 肾阳虚证动物实验研究简述及展望[J]. 中医药学刊, 2006,24(10):1809-1810
[5] Chen XY. Practical animal models of traditional Chinese medicine syndromes[M]. Beijing: Joint Publishing House of Peking Medical University and Peking Union Medical University, 1993: 100
陈小野 . 实用中医证候动物模型学(第2版)[M]. 北京: 北京医科大学中国协和医科大学联合出版社, 1993: 100
[6] Aikawa T, Kasahara T, Uchiyama M . Circadian variation of plasma arginine vasopressin concentration,or arginine vasopressin in enuresis[J]. Scand J UrolNephrol Supp, 1999,202:47-49
[7] Wang SC. Pediatrics of traditional Chinese medicine(7th ed)[M]. Beijing: China Press of Traditional Chinese Medicine, 2004: 13
汪受传 . 中医儿科学(第7版)[M]. 北京: 中国中医药出版社, 2004: 13
[8] Yang JY . Pathogenesis of nocturnal enuresis in children and progress in diagnosis and treatment[J]. Shi Yong Er Ke Lin Chuang Za Zhi, 2005,20(5):385
杨霁云 . 小儿夜间遗尿症发病机制及诊治进展[J]. 实用儿科临床杂志, 2005,20(5):385
[9] Deng HY, Gao Y, Ye H, Deng YM, Zhong F, Yang HB . Clinical observation of dry-bed training and Minirin treatment of children with primary enuresis[J]. Shi Yong Yi Xue Za Zhi, 2008,24(11):1928-1930
邓会英, 高岩, 叶红, 邓颖敏, 钟桴, 杨华彬 . 干床训练和弥凝治疗儿童原发性遗尿症的临床观察[J]. 实用医学杂志, 2008,24(11):1928-1930
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