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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (4): 373-378.doi: 10.3736/jcim20100413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Ginkgo biloba extract 50 preconditioning on contents of inflammation-related cytokines in myocardium of rats with ischemia-reperfusion injury

 Yi-min Baoa, Ai-hua Liua, Zhi-xiong Zhanga, Yun Lib, Xing-yu Wangb   

  1. a Department of Physiology, School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2010-01-06 Accepted:2010-02-24 Online:2010-04-20 Published:2010-04-15
  • Contact: Zhi-xiong Zhang E-mail:zixion@online.sh.cn

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Objective

To investigate the effects of Ginkgo biloba extract 50 (GBE50) preconditioning on contents of inflammation-related cytokines in rats with myocardial ischemia-reperfusion.
Methods

Fifty-eight SD rats were divided into sham-operated group, untreated group, Salviae miltiorrhizae (SM) injection group and low-, medium- and high-dose GBE50 groups. After intragastric administration for 7 d, the left anterior descending (LAD) coronary artery was occluded for 30 min followed by 60-min reperfusion to induce ischemia-reperfuion injury. Myocardium histopathologic change was observed by HE staining under a light microscope; myeloperoxidase (MPO) activity in myocardium was measured by colorimetric detection; tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and IL-8 were detected by radioimmunoassay; IL-4 and IL-10 were tested by enzyme-linked immunosorbent assay (ELISA).
Results

Compared with untreated group, rats in medium-dose GBE50 group had lower inflammatory reaction and MPO activity (P<0.01). GBE50 also decreased the content of IL-6 (P<0.05, P<0.01) and increased the content of IL-4 in myocardium (P<0.05, P<0.01) as compared with the untreated group. The content of TNF-α in myocardium in the medium-dose GBE50 group was lower and IL-10 was higher than those in the untreated group, but without significant differences.
Conclusion

GBE50 can decrease the content of IL-6 and increase the content of IL-4 in myocardium after ischemia-reperfusion injury. It suggests that GBE50 can regulate the inflammatory reaction after ischemia-reperfusion injury via inhibiting inflammatory cytokines and promoting anti-inflammatory cytokines.

Key words: Myocardial ischemia-reperfusion, GBE50, Lnterleukin-6, Lnterleukin-4, Tumor necrosis factor-alpha, Interleukin-10, Rats

Figure 1

Morphological changes of myocardium after IRI observed by HE staining (Light microscopy, ×400) A: Sham-operated group; B: Untreated group; C: SM injection group; D: Medium-dose GBE50 group."

Table 1

MPO activity in myocardium in different groups after IRI ($\bar{x}$±s, U/g)"

Group n Activity of MPO
Sham-operated 8 0.75±0.16
Untreated 8 1.37±0.30**
SM injection 8 1.10±0.17**△
Low-dose GBE50 8 1.29±0.25**
Medium-dose GBE50 8 0.97±0.21*△△▲▲
High-dose GBE50 8 1.11±0.13**△

Table 2

Contents of TNF-α, IL-6 , IL-8, IL-4 and IL-10 in myocardium in different groups after IRI ($\bar{x}$±s)"

Group n TNF-α (pg/mg) IL-6 (pg/mg) IL-8 (pg/mg) IL-4 (ng/L) IL-10 (ng/L)
Sham-operated 8 13.31±2.24 0.93±0.27 5.24±1.27 71.70±7.40 143.55±12.87
Untreated 8 22.42±6.54** 1.33±0.23** 6.13±1.18 70.29±2.98 149.70±13.36
SM injection 8 18.92±5.67* 1.04±0.28△ 5.97±1.13 80.58±7.75*△ 157.24±23.59
Low-dose GBE50 8 20.43±4.36** 1.01±0.26△ 6.00±1.12 74.45±6.94 161.11±26.65
Medium-dose GBE50 8 18.08±3.24* 1.04±0.22△ 5.77±1.28 80.86±8.31*△ 166.91±17.76*
High-dose GBE50 8 18.69±2.00* 0.92±0.10△△ 5.13±1.63 89.89±11.38**△△▲▲□■ 167.48±15.49*
[1] Jordan JE, Zhao ZQ, Vinten-Johansen J . The role of neutrophils in myocardial ischemia-reperfusion injury[J]. Cardiovasc Res, 1999,43(4):860-878
doi: 10.1016/S0008-6363(99)00187-X
[2] Vinten-Johansen J . Involvement of neutrophils in the pathogenesis of lethal myocardial reperfusion injury[J]. Cardiovasc Res, 2004,61(3):481-497
doi: 10.1016/j.cardiores.2003.10.011
[3] Rothenbacher D, Müller-Scholze S, Herder C, Koenig W, Kolb H . Differential expression of chemokines, risk of stable coronary heart disease, and correlation with established cardiovascular risk markers[J]. Arterioscler Thromb Vasc Biol, 2006,26(1):194-199
doi: 10.1161/01.ATV.0000191633.52585.14
[4] Boros P, Bromberg JS . New cellular and molecular immune pathways in ischemia/reperfusion injury[J]. Am J Transplant, 2006,6(4):652-658
doi: 10.1111/j.1600-6143.2005.01228.x
[5] Vinten-Johansen J, Jiang R, Reeves JG, Mykytenko J, Deneve J, Jobe LJ . Inflammation, proinflammatory mediators and myocardial ischemia-reperfusion injury[J]. Hematol Oncol Clin North Am, 2007,21(1):123-145
doi: 10.1016/j.hoc.2006.11.010
[6] Chang JJ, Pan JH . Effect of GBE50 on expressions of IL-1β and TNF-α in rat’s MCAO model[J]. Zhongguo Yao Li Tong Xun, 2003,20(3):42
常建杰, 潘家祜 . GBE50对大鼠MCAO模型上IL-1β, TNF-α表达的影响[J]. 中国药理通讯, 2003,20(3):42
[7] Wang SX, Lan XL, Wang JW, Pei ZG . Improving and evaluating the rat model of myocardial ischemia-reperfusion injury[J]. Jie Pou Ke Xue Jin Zhan, 2000,6(4):371-373
王淑侠, 兰小莉, 王吉文, 裴著果 . 大鼠心肌缺血/再灌注损伤模型的改进与评价[J]. 解剖科学进展, 2000,6(4):371-373
[8] Zhou WW, Lin L, Chen J, Yang JF, Zhou XM, Hu JG . Structure of ischemia heart model in rats by coronary artery ligation[J]. Zhongguo Shi Yan Dong Wu Xue Bao, 2004,12(4):226-230
周文武, 林玲, 陈军, 杨进福, 周新民, 胡建国 . 冠脉结扎法制做大鼠心肌缺血模型[J]. 中国实验动物学报, 2004,12(4):226-230
[9] Chen X, Ao JN . Discussion about traditional Chinese medicine pathogenesis of myocardial ischemia-reperfusion and its prevention and treatment[J]. Shaanxi Zhong Yi, 2008,29(6):697-699
陈相, 敖杰男 . 浅谈心肌缺血再灌注的中医病机及其防治[J]. 陕西中医, 2008,29(6):697-699
[10] Jiang SM, Fu Y, Chen YP, Kou H, Ji H, Zhao LQ, Zhao LJ, Huang Y, Wang XF . Study on protective effects of traditional Chinese medical complex prescription on myocardial ischemia/reperfusion injury after coronary artery ligation in rats[J]. Zhongguo Zhong Xi Yi Jie He Ji Jiu Za Zhi, 2005,12(6):347-350
姜树民, 付瑜, 陈颖萍, 寇辉, 汲鸿, 赵立群, 赵丽君, 黄煜, 王晓凤 . 中药复方对大鼠冠状动脉结扎后再灌注心肌损伤保护作用的研究[J]. 中国中西医结合急救杂志, 2005,12(6):347-350
[11] Shi ZX . Think of the Chinese integrative therapy in the era of reperfusion challenge[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2005,3(1):2-5
史载祥 . 后再灌注时代难题的中西医结合治疗思考[J]. 中西医结合心脑血管病杂志, 2005,3(1):2-5
[12] Linfert D, Chowdhry T, Rabb H . Lymphocytes and ischemia-reperfusion injury[J]. Transplant Rev (Orlando), 2009,23(1):1-10
doi: 10.1016/j.trre.2008.08.003
[13] Frangogiannis NG . The immune system and cardiac repair[J]. Pharmacol Res, 2008,58(2):88-111
doi: 10.1016/j.phrs.2008.06.007
[14] Frangogiannis NG, Youker KA, Rossen RD, Gwechenberger M, Lindsey MH, Mendoza LH, Michael LH, Ballantyne CM, Smith CW, Entman ML . Cytokines and the microcirculation in ischemia and reperfusion[J]. J Mol Cell Cardiol, 1998,30(12):2567-2576
doi: 10.1006/jmcc.1998.0829
[15] Frangogiannis NG, Lindsey ML, Michael LH, Youker KA, Bressler RB, Mendoza LH, Spengler RN, Smith CW, Entman ML . Resident cardiac mast cells degranulate and release preformed TNF alpha, initiating the cytokine cascade in experimental canine myocardial ischemia/reperfusion[J]. Circulation, 1998,98(7):699-710
doi: 10.1161/01.CIR.98.7.699
[16] Han NL, Dai XH, Zhou YX, Yu H, Xu XX . The influence of the therapy invigorating qi and activating blood circulation on the levels of IL-6 and TNF-α of rats with ischemia and reperfusion injury of heart[J]. Zhongguo Zhong Yi Ji Zheng, 2005,14(5):456-458
韩宁林, 戴小华, 周宜轩, 俞浩, 徐先祥 . 益气活血法对心肌缺血-再灌注损伤大鼠IL-6及TNF-α含量的影响[J]. 中国中医急症, 2005,14(5):456-458
[17] Huang ZD, Li XH, Xie XJ, Zhang SQ . Influence of drugs for activating microcirculation and removing stasis on the endothelial vaso-active factors and inflammatory factors of myocardial ischemia-reperfusion injury in blood stasis syndrome of rabbits[J]. Zhonghua Zhong Yi Yao Xue Kan, 2007,25(7):1319-1321
黄政德, 李鑫辉, 谢雪姣, 张少泉 . 活血化瘀药对血瘀证心肌缺血再灌注损伤家兔内源性活性因子及炎症因子的影响[J]. 中华中医药学刊, 2007,25(7):1319-1321
[18] Xu Y, Zhang ZX, Li Y, Wang XY . Effects of Ginkgo biloba extract on learning and memory and cytokines of hippocampus in senile rats[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2008,22(3):51-54
徐颖, 张志雄, 李云, 王星禹 . 银杏酮酯对衰老模型大鼠学习记忆及海马细胞因子的影响[J]. 上海中医药大学学报, 2008,22(3):51-54
[19] Xiong N, Sun F, Zhao H, Xiang J . Effect of Rosiglitazone Maleate on inflammation following cerebral ischemia/reperfusion in rats[J]. J Huazhong Univ Sci Technolog Med Sci, 2007,27(3):295-298
doi: 10.1007/s11596-007-0320-x
[20] Samimi-Fard S, Dominguez-Rodriguez A, Abreu-Gonzalez P, Enjuanes-Grau C, Blanco-Palacios G, Hernandez-Baldomero IF, Bosa-Ojeda F, Marrero-Rodríguez F . Role of myeloperoxidase as predictor of systemic inflammatory response syndrome in patients with ST-segment elevation myocardial infarction after primary percutaneous coronary intervention[J]. Am J Cardiol, 2009,104(5):634-637
doi: 10.1016/j.amjcard.2009.04.049
[21] Karadag B, Vatan B, Hacioglu Y, Duman D, Baskurt M, Keles I, Ongen Z, Vural VA . Serum myeloperoxidase level predicts reperfusion in patients with myocardial infarction receiving thrombolytic therapy[J]. Heart Vessels, 2009,24(4):247-253
doi: 10.1007/s00380-008-1115-0
[22] .[ OTHER].Zhang C, Wu J, Xu X, Potter BJ, Gao X. Direct relationship between levels of TNF-alpha expression and endothelial dysfunction in reperfusion injury. Basic Res Cardiol. 2010 Jan 21. [Epub ahead of print],,( ):
[23] Niemann JT, Rosborough JP, Younqquist S, Shah AP, Lewis RJ, Phan QT, Filler SG . Cardiac function and the proinflammatory cytokine response after recovery from cardiac arrest in swine[J]. J Interferon Cytokine Res, 2009,29(11):749-758
doi: 10.1089/jir.2009.0035
[24] Vanden Berghe W, Vermeulen L, De Wilde G, De Bosscher K, Boone E, Haegeman G . Signal transduction by tumor necrosis factor and gene regulation of the inflammatory cytokine interleukin-6[J]. Biochem Pharmacol, 2000,60(8):1185-1195
doi: 10.1016/S0006-2952(00)00412-3
[25] Dhingra S, Sharma AK, Arora RC, Slezak J, Singal PK . IL-10 attenuates TNF-alpha-induced NF kappaB pathway activation and cardiomyocyte apoptosis[J]. Cardiovasc Res, 2009,82(1):59-66
doi: 10.1093/cvr/cvp040
[26] Sheng XL, Wang DM, Chen C . The changes of endogenous interleukin-10, NOS and iNOS during myocardial ischemia and reperfusion in rats[J]. Zhongguo Yi Shi Za Zhi, 2005,7(12):1619-1621
盛西陵, 王东明, 陈畅 . 大鼠心肌缺血再灌注IL-10、NOS、iNOS的变化[J]. 中国医师杂志, 2005,7(12):1619-1621
[27] Frangogiannie NG, Mendoza LH, Lindsey ML, Ballantyne CM, Michael LH, Smith CW, Entman ML . IL-10 is induced in the reperfused myocardium and may modulate the reaction to injury[J]. J Immunol, 2000,165(5):2798-2808
doi: 10.4049/jimmunol.165.5.2798
[28] Moro C, Jouan MG, Rakotovao A, Toufektsian MC, Ormezzano O, Nagy N , Tosaki A, de Leiris J, Boucher F .Delayed expression of cytokines after reperfused myocardial infarction: possible trigger for cardiac dysfunction and ventricular remodeling[J]. Am J Physiol Heart Circ Physiol, 2007,293(5):H3014-H3019
doi: 10.1152/ajpheart.00797.2007
[29] Kaur K, Dhingra S, Slezak J, Sharma AK, Bajaj A, Singal PK . Biology of TNFalpha and IL-10, and their imbalance in heart failure[J]. Heart Fail Rev, 2009,14(2):113-123
doi: 10.1007/s10741-008-9104-z
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