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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (4): 368-372.doi: 10.3736/jcim20100412

• Original Experimental Research • Previous Articles     Next Articles

Effects of Radix Ginseng and Radix Notoginseng formula on secretion of vascular endothelial growth factor and expression of vascular endothelial growth factor receptor-2 in human umbilical vein endothelial cells

Yan Leia, Wei Tiana, Lin-qun Zhub, Jing Yanga, Ke-ji Chena   

  1. a Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    b The Key Laboratory of Chinese Internal Medicine of Ministry of Education, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2010-01-08 Accepted:2010-03-01 Online:2010-04-20 Published:2010-04-15
  • Contact: Yan Lei E-mail:leiy999@163.com

Objective

To investigate the effects of Radix Ginseng and Radix Notoginseng formula on secretion of vascular endothelial growth factor (VEGF) and expression of vascular endothelial growth factor receptor-2 (VEGFR-2) in human umbilical vein endothelial cells (HUVECs) in vitro.
Methods

HUVECs were cultured in vitro. Bovine basic fibroblast growth factor (bFGF) at concentration of 320 U/mL and Radix Ginseng and Radix Notoginseng formula at concentrations of 0.1, 0.2 and 0.4 mg/mL were used to culture with HUVECs. And HUVECs in blank control group were cultured with culture solution only. After 24-hour culture, the content of VEGF in supernatant was detected by enzyme-linked immunosorbent assay and the expression of VEGFR-2 was detected by immunocytochemical staining and Western-blotting.
Results

Radix Ginseng and Radix Notoginseng formula at 0.4 mg/mL, the same as bFGF, increased VEGF content in the HUVEC supernatant and the number of VEGFR-2-positive HUVECs. Expression of VEGFR-2 protein in high-dose Radix Ginseng and Radix Notoginseng formula group was up-regulated as compared with the blank control group.
Conclusion

Radix Ginseng and Radix Notoginseng formula can promote HUVEC proliferation and secretion of VEGF, as well as the expression of VEGFR-2 protein, which may be one of the mechanisms of Radix Ginseng and Radix Notoginseng formula in promoting angiogenesis.

Key words: Drugs, Chinese herbal, Vascular endothelial growth factor, Vascular endothelial growth factor receptor-2, Endothelial cell

Table 1

Content of VEGF, number of VEGFR-2-positive HUVECs and expression of VEGFR-2 protein in different groups ($\bar{x}$±s)"

Group VEGF (ng/L,n=8) Number of
VEGFR-2-positive
HUVECs (n=6)
OD value of
VEGFR-2-positive
HUVECs (n=6)
VEGFR-2/β-actin
(n=3)
Blank control 67.61±8.54 13.18±5.79 0.149±0.002 1.583 9±0.102 8
bFGF 93.68±11.35* 18.08±6.06** 0.183±0.002* 2.113 9±0.211 5*
High-dose formula 89.24±12.16* 18.29±4.98** 0.171±0.003* 2.074 5±0.189 8*
Medium-dose formula 74.57±8.90 13.81±4.31△△□□ 0.168±0.003* 1.482 2±0.173 0
Low-dose formula 72.81±9.26 12.79±4.10△△□□ 0.147±0.003△▲□ 1.388 8±0.262 0

Figure 1

Expression of VEGFR-2 protein in HUVECs observed by Western blotting A: Blank control group; B: bFGF group; C: High-dose formula group; D: Medium-dose formula group; E: Low-dose formula group."

Figure 2

Expression of VEGFR-2 protein in HUVECs observed by immunocytochemical method (Light microscopy, ×200) A: Blank control group; B: bFGF group; C: High-dose formula group; D: Medium-dose formula group; E: Low-dose formula group."

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