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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 548-553.doi: 10.3736/jcim20100607

• Original Experimental Research • Previous Articles     Next Articles

Effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 and hypoxia-inducible factor-1α in ischemic myocardium of rats with acute myocardial infarction

Xue-jun Du,Yan Lei(),Jing Yang   

  1. Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2010-02-23 Accepted:2010-03-24 Online:2010-06-20 Published:2010-06-15
  • Contact: Yan Lei E-mail:leiy999@163.com

Objective

To investigate the effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 (VEGFR-2) and hypoxia-inducible factor-1α (HIF-1α) in ischemic myocardium of rats with acute myocardial infarction.
Methods

A total of 100 Wistar rats were randomly divided into normal control group, sham-operated group, untreated group, metoprolol (Betaloc) group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups. Acute myocardial infarction was induced in the untreated group, Betaloc group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups by left anterior descending coronary artery ligation. After 12-day treatment, microvessel density (MVD) in ischemic myocardium was detected by immunohistochemical method, while expressions of VEGFR-2 and HIF-1α proteins were detected by Western blotting, and expressions of VEGFR-2 and HIF-1α mRNAs were detected by real-time fluorescent quantitative polymerase chain reaction.
Results

MVD in the untreated group was increased significantly, higher than those in the normal control group and the sham-operated group (P<0.05) and lower than those in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group (P<0.01). VEGFR-2 and HIF-1α protein and mRNA expressions in the untreated group were higher than those in the normal control group and the sham-operated group (P<0.05). VEGFR-2 and HIF-1α protein and mRNA expressions in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group were higher than those in the untreated group (P<0.05). There was a significant difference between the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups (P<0.05).
Conclusion

Radix Ginseng and Radix Notoginseng extract can up-regulate the protein and mRNA expressions of VEGFR-2 and HIF-1α and increase MVD in ischemic myocardium to improve myocardial ischemia so as to promote the development of collateral circulation.

Key words: Radix Ginseng, Radix Notoginseng , Vascular endothelial growth factor receptor-2, Hypoxia- inducible factor-la, Myocardial ischemia, Rats

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Figure 1

Expressions of VEGFR-2 and HIF-1α proteins in myocardium observed by Western blotting1: Normal control group; 2: Sham-operated group; 3: Untreated group; 4: Bataloc group; 5: High-dose formula group; 6: Low-dose formula group."

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