[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 979-984.doi: 10.3736/jcim20101011

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture at Shuigou (DU26) point on motor evoked potential in rats with cerebral infarction

 Wen-ping Yaoa, Shu Wangb,c, Lin Hana, Jin-quan Mab,Yan Shenb,c   

  1. a Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    b Institute of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    c State Administration of Traditional Chinese Medicine Key Laboratory of Encephalopathy Acupuncture, Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin 300193, China
  • Received:2010-03-09 Accepted:2010-05-19 Online:2010-10-20 Published:2010-10-15

RichHTML

1

PDF (PC)

130

Like

Objective

To investigate the effects of electro-acupuncture at Shuigou (DU26) on latency and amplitude of motor evoked potential (MEP) in rats after cerebral infarction.
Methods

Fifty healthy male Wistar rats were randomly divided into five groups as normal group, untreated group, sham-operated group, non-acupoint group and Shuigou group, with 10 rats in each group. By using a modification of the technique of middle cerebral artery occlusion, focal cerebral ischemia injury was induced in rats except normal and sham-operated groups. Rats in the normal group received no treatment. Rats in the sham-operated group underwent identical experimental procedures as the other groups, except that the nylon suture was inserted into the internal carotid artery. After the behavioral deficit was evaluated by using Zausinger 6-point neurological function score, rats in the Shuigou group and non-acupoint group were separately acupunctured and stimulated by continuous-wave with 2 Hz frequency, 1 mA intensity, for 10 minutes, twice a day for 3 days. Non-acupoint was located at the left side 0.5 cm of the Shuigou point. No intervention was given to the other groups. Zausinger 6-point neurological function and MEP were measured after 72-hour treatment.
Results

The latency at the affected side in the untreated group was significantly longer than that in the normal group (P<0.05), and the amplitude at the affected side was significantly reduced (P<0.01). After 3-day electro-acupuncture, the latency at the affected side of rats in the Shuigou group was obviously shortened as compared with the untreated group (P<0.05), and the amplitude was significantly increased (P<0.05). The latency at the affected side in the Shuigou group was shorter than that in the non-acupoint group (P<0.05), and the amplitude tended to increase as compared with the non-acupoint group, but there were no significant differences (P>0.05). There were no significant differences on the latency and amplitude between the untreated group and non-acupoint group (P>0.05). The neurobehavioral score of the Shuigou group was significantly higher than those of the untreated group and non-acupoint group (P<0.05), and there were no significant differences between the untreated group and non-acupoint group (P>0.05).
Conclusion

Electro-acupuncture at Shuigou can increase the latency and amplitude of MEP in rats after cerebral infarction.

Key words: Electro-acupuncture, Shuigou, Cerebral infarction, Motor evoked potential, Rats

Table 1

Neurobehavioral scores of rats in different groups"

Table 2

MEP latency of rats in different groups"

Table 3

MEP amplitude of rats in different groups"

Figure 1

MEP of rats in different groups A: Normal group; B: The affected side of sham-operated group; C: The affected side of untreated group; D: The affected side of non-acupoint group; E: The affected side of Shuigou group."

[1] Escudero JV, Sancho J, Bautista D, Escudero M, López-Trigo J . Prognostic value of motor evoked potential obtained by transcranial magnetic brain stimulation in motor function recovery in patients with acute ischemic stroke[J]. Stroke, 1998,29(9):1854-1859
doi: 10.1161/01.STR.29.9.1854
[2] Cruz Martínez A, Muñoz J, Palacios F .The muscle inhibitory period by transcranial magnetic stimulation. Study in stroke patients[J]. Electromyogr Clin Neurophysiol, 1998,38(3):189-192
pmid: 9637946
[3] Schäbitz WR, Schade H, Heiland S, Kollmar R, Bardutzky J, Henninger N, Müller H, Carl U, Toyokuni S, Sommer C, Schwab S . Neuroprotection by hyperbaric oxygenation after experimental focal cerebral ischemia monitored by MRI[J]. Stroke, 2004,35(5):1175-1179
doi: 10.1161/01.STR.0000125868.86298.8e
[4] Huang W, Wu XP, Ding J, Wang YW, Wang XJ, Sun GJ . Influence of electro-acupuncture of “Shuigou”(GV26) on hippocampal CGRP and NPY contents in cerebral ischemia rats[J]. Zhen Ci Yan Jiu, 2009,34(1):13-15, 42
黄伟, 吴绪平, 丁昀, 王亚文, 王述菊, 孙国杰 . 电针“水沟”对脑缺血大鼠海马降钙素基因相关肽、神经肽Y含量的影响[J]. 针刺研究, 2009,34(1):13-15, 42
[5] Yang ZJ, Camporesi C, Yang X, Wang J, Bosco G, Lok J, Gorji R, Schelper RL, Camporesi EM . Hyperbaric oxygenation mitigates focal cerebral injury and reduces striatal dopamine release in a rat model of transient middle cerebral artery occlusion[J]. Eur J Appl Physiol, 2002,87(2):101-107
doi: 10.1007/s00421-002-0601-9
[6] Longa EZ, Weinstein PR, Carlson S, Cummins R . Reversible middle cerebral artery occlusion without craniotomy in rats[J]. Stroke, 1989,20(1):84-91
doi: 10.1161/01.STR.20.1.84
[7] Zausinger S, Hungerhuber E, Baethmann A, Reulen H, Schmid-Elsaesser R . Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms[J]. Brain Res, 2000,863(1-2):94-105
doi: 10.1016/S0006-8993(00)02100-4
[8] Li ZR. Experimental acupuncture science[M]. Beijing: China Press of Traditional Chinese Medicine,2003: 327-328
李忠仁 . 实验针灸学[M]. 北京: 中国中医药出版社,2003: 327-328
[9] Yang XG, Li Y, Tian XP, Liang FR . Review on acupuncture study of non-meridian and non-point selection method all over the world[J]. Zhong Yi Za Zhi, 2009,50(8):748-750
doi: 10.3321/j.issn:1001-1668.2009.08.033
杨旭光, 李瑛, 田小平, 梁繁荣 . 国内外针灸研究中非经非穴选取方法评述[J]. 中医杂志, 2009,50(8):748-750
doi: 10.3321/j.issn:1001-1668.2009.08.033
[10] Bao XM, Shu SY. Stereotaxic atlas of rat brain[M]. Beijing: People’s Medical Publishing House,1991: 21-29
包新民, 舒斯云 . 大鼠脑立体定位图谱[M]. 北京: 人民卫生出版社,1991: 21-29
[11] Abbruzzese G, Morena M, Dall’Agata D, Abbruzzese M, Favale E .Motor evoked potentials(MEPs) in lacunar syndromes[J]. Electroencephalogr Clin Neurophysiol, 1991,81(3):202-208
doi: 10.1016/0168-5597(91)90073-7 pmid: 1710969
[12] Yu KW, Li JS, Jia LS, Yuan W, Hu YH, Shi ZC, Dai BJ, He HL . Study on the amplitude and latency characteristics of motor evoked potentials elicited by transcranial magnetic or electric stimulation in rats[J]. Di Er Jun Yi Da Xue Xue Bao, 2000,21(7):625-628
doi: 10.3321/j.issn:0258-879X.2000.07.008
余科炜, 李家顺, 贾连顺, 袁文, 胡玉华, 石志才, 戴伯军, 何海龙 . 大鼠经颅磁及电刺激运动诱发电位波幅及潜伏期特征的研究[J]. 第二军医大学学报, 2000,21(7):625-628
doi: 10.3321/j.issn:0258-879X.2000.07.008
[13] Kovala T, Tolonen U, Pyhtinen J . Median nerve and posterior tibial nerve somatosensory evoked potentials in the non-affected hemisphere: a prospective one-year follow-up study in patients with supratentorial cerebral infarction[J]. Electromyogr Clin Neurophysiol, 1991,31(2):85-92
[14] Yang YH, Wu XP, Liu YX, Sun GJ . Analysis of brain CT images for electro-acupuncture treatment of ischemic and hemorrhagic apoplexy at early stage[J]. Zhongguo Zhen Jiu, 1999,19(6):325-327
杨毅红, 吴绪平, 刘又香, 孙国杰 . 早期电针治疗缺血性与出血性中风头颅CT分析[J]. 中国针灸, 1999,19(6):325-327
[15] Guo JC, Cheng JS . Effect of electro-acupuncture on extracellular contents of excitatory amino acid transmitters in rat striatum after transient focal cerebral ischemia[J]. Shanghai Zhen Jiu Za Zhi, 2000,19(1):37-39
郭景春, 程介士 . 电针对脑缺血大鼠纹状体细胞外兴奋性氨基酸水平的影响[J]. 上海针灸杂志, 2000,19(1):37-39
[16] Zhan XL, Pan SQ, Su BG, Lu LQ, Ma SF . Up-regulation of electroacupuncture on the expression of neuroglobin in cortical penumbra of MCAO rats[J]. Zhongguo Lin Chuang Jie Pou Xue Za Zhi, 2009,27(3):313-315
湛湘丽, 潘三强, 宿宝贵, 吕来清, 马尚峰 . 电针上调MCAO大鼠皮质缺血半暗带脑红蛋白的表达[J]. 中国临床解剖学杂志, 2009,27(3):313-315
[1] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[2] Lawrence A. Olatunji, Taofeek O. Usman, Joseph O. Adebayo, Victoria A. Olatunji . Effects of aqueous extract of Hibiscus sabdariffa on renal Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in Wistar rats. Journal of Chinese Integrative Medicine, 2012, 10(9): 1049-1055.
[3] Dhirender Kaushik, Ajay Kumar, Pawan Kaushik, Avatar C. Rana. Anticonvulsant activity of alcoholic extract of bark of Pinus roxburghii Sarg. Journal of Chinese Integrative Medicine, 2012, 10(9): 1056-1060.
[4] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[5] Jian-sheng Li, Ya Li, Su-yun Li, Yuan-yuan Wang, Li Deng, Yan-ge Tian, Su-li Jiang , Ying Wang. Long-term effects of Tiaobu Feishen therapies on systemic and local inflammation responses in rats with stable chronic obstructive pulmonary disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1039-1048.
[6] Karuna A. Talpate, Uma A. Bhosale, Mandar R. Zambare. Clitorea ternatea, a herb from Indian folklore, improves streptozotocin-induced diabetes and diabetes-induced cognitive decline in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 939-947.
[7] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[8] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[9] Meng Li, Ling Hu, Rong-lin Cai, Zi-jian Wu, Ke-ming Wang. Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia. Journal of Chinese Integrative Medicine, 2012, 10(8): 874-879.
[10] Xin-min Li, Hui Chen, Yan-yan Ren, Yan-li Lu. Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy. Journal of Chinese Integrative Medicine, 2012, 10(8): 911-917.
[11] Hong Wang , Jing-tian Ou , Xin Zhao , Ying Guoz , Hao-ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 880-885.
[12] C. Mouli Kalla, Vijaya Tartte, Dattatreya Rao Sirpurkar. Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence. Journal of Chinese Integrative Medicine, 2012, 10(7): 784-792.
[13] Feng Xin-ying, Zhu Min, Zhang Qi-qi, Chen Yi-ping, Li Wen-wei. Selective protection of nigral dopaminergic neurons by echinacoside in a rat model of Parkinson disease induced by rotenone. Journal of Chinese Integrative Medicine, 2012, 10(7): 777-783.
[14] Chuan Chen, Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen . Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(6): 667-673.
[15] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]