Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 979-984.doi: 10.3736/jcim20101011

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture at Shuigou (DU26) point on motor evoked potential in rats with cerebral infarction

 Wen-ping Yaoa, Shu Wangb,c, Lin Hana, Jin-quan Mab,Yan Shenb,c   

  1. a Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    b Institute of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    c State Administration of Traditional Chinese Medicine Key Laboratory of Encephalopathy Acupuncture, Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin 300193, China
  • Received:2010-03-09 Accepted:2010-05-19 Online:2010-10-20 Published:2010-10-15

Objective

To investigate the effects of electro-acupuncture at Shuigou (DU26) on latency and amplitude of motor evoked potential (MEP) in rats after cerebral infarction.
Methods

Fifty healthy male Wistar rats were randomly divided into five groups as normal group, untreated group, sham-operated group, non-acupoint group and Shuigou group, with 10 rats in each group. By using a modification of the technique of middle cerebral artery occlusion, focal cerebral ischemia injury was induced in rats except normal and sham-operated groups. Rats in the normal group received no treatment. Rats in the sham-operated group underwent identical experimental procedures as the other groups, except that the nylon suture was inserted into the internal carotid artery. After the behavioral deficit was evaluated by using Zausinger 6-point neurological function score, rats in the Shuigou group and non-acupoint group were separately acupunctured and stimulated by continuous-wave with 2 Hz frequency, 1 mA intensity, for 10 minutes, twice a day for 3 days. Non-acupoint was located at the left side 0.5 cm of the Shuigou point. No intervention was given to the other groups. Zausinger 6-point neurological function and MEP were measured after 72-hour treatment.
Results

The latency at the affected side in the untreated group was significantly longer than that in the normal group (P<0.05), and the amplitude at the affected side was significantly reduced (P<0.01). After 3-day electro-acupuncture, the latency at the affected side of rats in the Shuigou group was obviously shortened as compared with the untreated group (P<0.05), and the amplitude was significantly increased (P<0.05). The latency at the affected side in the Shuigou group was shorter than that in the non-acupoint group (P<0.05), and the amplitude tended to increase as compared with the non-acupoint group, but there were no significant differences (P>0.05). There were no significant differences on the latency and amplitude between the untreated group and non-acupoint group (P>0.05). The neurobehavioral score of the Shuigou group was significantly higher than those of the untreated group and non-acupoint group (P<0.05), and there were no significant differences between the untreated group and non-acupoint group (P>0.05).
Conclusion

Electro-acupuncture at Shuigou can increase the latency and amplitude of MEP in rats after cerebral infarction.

Key words: Electro-acupuncture, Shuigou, Cerebral infarction, Motor evoked potential, Rats

Table 1

Neurobehavioral scores of rats in different groups"

Table 2

MEP latency of rats in different groups"

Table 3

MEP amplitude of rats in different groups"

Figure 1

MEP of rats in different groups A: Normal group; B: The affected side of sham-operated group; C: The affected side of untreated group; D: The affected side of non-acupoint group; E: The affected side of Shuigou group."

[1] Escudero JV, Sancho J, Bautista D, Escudero M, López-Trigo J . Prognostic value of motor evoked potential obtained by transcranial magnetic brain stimulation in motor function recovery in patients with acute ischemic stroke[J]. Stroke, 1998,29(9):1854-1859
doi: 10.1161/01.STR.29.9.1854
[2] Cruz Martínez A, Muñoz J, Palacios F .The muscle inhibitory period by transcranial magnetic stimulation. Study in stroke patients[J]. Electromyogr Clin Neurophysiol, 1998,38(3):189-192
pmid: 9637946
[3] Schäbitz WR, Schade H, Heiland S, Kollmar R, Bardutzky J, Henninger N, Müller H, Carl U, Toyokuni S, Sommer C, Schwab S . Neuroprotection by hyperbaric oxygenation after experimental focal cerebral ischemia monitored by MRI[J]. Stroke, 2004,35(5):1175-1179
doi: 10.1161/01.STR.0000125868.86298.8e
[4] Huang W, Wu XP, Ding J, Wang YW, Wang XJ, Sun GJ . Influence of electro-acupuncture of “Shuigou”(GV26) on hippocampal CGRP and NPY contents in cerebral ischemia rats[J]. Zhen Ci Yan Jiu, 2009,34(1):13-15, 42
黄伟, 吴绪平, 丁昀, 王亚文, 王述菊, 孙国杰 . 电针“水沟”对脑缺血大鼠海马降钙素基因相关肽、神经肽Y含量的影响[J]. 针刺研究, 2009,34(1):13-15, 42
[5] Yang ZJ, Camporesi C, Yang X, Wang J, Bosco G, Lok J, Gorji R, Schelper RL, Camporesi EM . Hyperbaric oxygenation mitigates focal cerebral injury and reduces striatal dopamine release in a rat model of transient middle cerebral artery occlusion[J]. Eur J Appl Physiol, 2002,87(2):101-107
doi: 10.1007/s00421-002-0601-9
[6] Longa EZ, Weinstein PR, Carlson S, Cummins R . Reversible middle cerebral artery occlusion without craniotomy in rats[J]. Stroke, 1989,20(1):84-91
doi: 10.1161/01.STR.20.1.84
[7] Zausinger S, Hungerhuber E, Baethmann A, Reulen H, Schmid-Elsaesser R . Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms[J]. Brain Res, 2000,863(1-2):94-105
doi: 10.1016/S0006-8993(00)02100-4
[8] Li ZR. Experimental acupuncture science[M]. Beijing: China Press of Traditional Chinese Medicine,2003: 327-328
李忠仁 . 实验针灸学[M]. 北京: 中国中医药出版社,2003: 327-328
[9] Yang XG, Li Y, Tian XP, Liang FR . Review on acupuncture study of non-meridian and non-point selection method all over the world[J]. Zhong Yi Za Zhi, 2009,50(8):748-750
doi: 10.3321/j.issn:1001-1668.2009.08.033
杨旭光, 李瑛, 田小平, 梁繁荣 . 国内外针灸研究中非经非穴选取方法评述[J]. 中医杂志, 2009,50(8):748-750
doi: 10.3321/j.issn:1001-1668.2009.08.033
[10] Bao XM, Shu SY. Stereotaxic atlas of rat brain[M]. Beijing: People’s Medical Publishing House,1991: 21-29
包新民, 舒斯云 . 大鼠脑立体定位图谱[M]. 北京: 人民卫生出版社,1991: 21-29
[11] Abbruzzese G, Morena M, Dall’Agata D, Abbruzzese M, Favale E .Motor evoked potentials(MEPs) in lacunar syndromes[J]. Electroencephalogr Clin Neurophysiol, 1991,81(3):202-208
doi: 10.1016/0168-5597(91)90073-7 pmid: 1710969
[12] Yu KW, Li JS, Jia LS, Yuan W, Hu YH, Shi ZC, Dai BJ, He HL . Study on the amplitude and latency characteristics of motor evoked potentials elicited by transcranial magnetic or electric stimulation in rats[J]. Di Er Jun Yi Da Xue Xue Bao, 2000,21(7):625-628
doi: 10.3321/j.issn:0258-879X.2000.07.008
余科炜, 李家顺, 贾连顺, 袁文, 胡玉华, 石志才, 戴伯军, 何海龙 . 大鼠经颅磁及电刺激运动诱发电位波幅及潜伏期特征的研究[J]. 第二军医大学学报, 2000,21(7):625-628
doi: 10.3321/j.issn:0258-879X.2000.07.008
[13] Kovala T, Tolonen U, Pyhtinen J . Median nerve and posterior tibial nerve somatosensory evoked potentials in the non-affected hemisphere: a prospective one-year follow-up study in patients with supratentorial cerebral infarction[J]. Electromyogr Clin Neurophysiol, 1991,31(2):85-92
[14] Yang YH, Wu XP, Liu YX, Sun GJ . Analysis of brain CT images for electro-acupuncture treatment of ischemic and hemorrhagic apoplexy at early stage[J]. Zhongguo Zhen Jiu, 1999,19(6):325-327
杨毅红, 吴绪平, 刘又香, 孙国杰 . 早期电针治疗缺血性与出血性中风头颅CT分析[J]. 中国针灸, 1999,19(6):325-327
[15] Guo JC, Cheng JS . Effect of electro-acupuncture on extracellular contents of excitatory amino acid transmitters in rat striatum after transient focal cerebral ischemia[J]. Shanghai Zhen Jiu Za Zhi, 2000,19(1):37-39
郭景春, 程介士 . 电针对脑缺血大鼠纹状体细胞外兴奋性氨基酸水平的影响[J]. 上海针灸杂志, 2000,19(1):37-39
[16] Zhan XL, Pan SQ, Su BG, Lu LQ, Ma SF . Up-regulation of electroacupuncture on the expression of neuroglobin in cortical penumbra of MCAO rats[J]. Zhongguo Lin Chuang Jie Pou Xue Za Zhi, 2009,27(3):313-315
湛湘丽, 潘三强, 宿宝贵, 吕来清, 马尚峰 . 电针上调MCAO大鼠皮质缺血半暗带脑红蛋白的表达[J]. 中国临床解剖学杂志, 2009,27(3):313-315
[1] Xin-fang Zhang, Shui-ying Xiang, Wen-ye Geng, Wen-juan Cong, Jing Lu, Chuan-wei Jiang, Kun Wang, Zi-bing Liu . Electro-acupuncture regulates the cholinergic anti-inflammatory pathway in a rat model of chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2018, 16(6): 418-426.
[2] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[3] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[4] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[5] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[8] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[9] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[12] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[13] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[14] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[15] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[2] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[3] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[4] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246
[5] Zhao-guo Li , Rui-qiang Liu. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 7). Journal of Chinese Integrative Medicine, 2012, 10(11): 1310-1315
[6] Yong Miao Edwin. Clinical critical qualitative evaluation of the selected randomized controlled trials in current acupuncture researches for low back pain. Journal of Chinese Integrative Medicine, 2010, 8(12): 1133-1146
[7] Le-qin Xu, Xiao-feng Li , You-wei Zhang , Bing Shu , Qi Shi, Yong-jun Wang, Chong-jian Zhou. Effects of Chinese herbal medicine Yiqi Huayu Recipe on apoptosis of dorsal root ganglion neurons and expression of caspase-3 in rats with lumbar nerve root compression. Journal of Chinese Integrative Medicine, 2010, 8(12): 1174-1179
[8] Jia Guo, Li-na Wang, Dong Li. Exploring the effects of Chinese medicine in improving uterine endometrial blood flow for increasing the successful rate of in vitro fertilization and embryo transfer. Journal of Chinese Integrative Medicine, 2011, 9(12): 1301-1306
[9] Li Dong-tao, Li Fu-yu, Wang Jian, Liu Ji-hong, Yan Nan, Cheng Yong-mei, Hu Ai-hua, Jiang Hong-yang, Shi Feng-lei, Zhang Mei-zeng, Li Jie, Wei Ling-bo, Jiang Rong-qin. A study of diagnostic criteria for traditional Chinese medicine syndromes in osteoporosis. Journal of Chinese Integrative Medicine, 2011, 9(12): 1326-1332
[10] Xing Liao, Yan-ming Xie. Application of qualitative interviews in clinical research of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(2): 119-123