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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (10): 1118-1124.doi: 10.3736/jcim20111013

• Original Experimental Research • Previous Articles     Next Articles

Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice

Mahmoud Hosseini1(), Mahboobeh Ghasemzadeh Rahbardar1, Hamid Reza Sadeghnia2, Hassan Rakhshandeh3#br#   

  1. 1. Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    2. Neuroscience Research Center and Department of Pharmacology, Department of New Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    3. Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Received:2011-05-05 Accepted:2011-08-07 Online:2011-10-20 Published:2011-10-15
  • Contact: Hosseini Mahmoud E-mail:hosseinim@mums.ac.ir

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Objective: In the present study, the effects of aqueous, ethanolic and chloroformic extracts of Rosa damascena on pentylenetetrazol (PTZ)-induced seizures were investigated in mice.

Methods: The animals were divided into the following groups: normal saline control group, diazepam group (3 mg/kg), three aqueous extract groups (100, 500 and 1 000 mg/kg), three ethanolic extract groups (100, 500 and 1 000 mg/kg) and three chloroformic extract groups (100, 500 and 1 000 mg/kg). The extracts, normal saline or diazepam were injected intraperitoneally 30 min before PTZ injection. Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality of rats in each group were recorded.

Results: Significant increases in both MCS and GTCS latencies were observed in all the three aqueous extract groups in comparison with the normal saline control group (P<0.05 or P<0.01). The MCS latency in the ethanolic extract (1 000 mg/kg) group and the GTCS latencies in the two ethanolic extract (500 and 1 000 mg/kg) groups were higher than those in the normal saline control group (P<0.05, P<0.01). There were no significant differences in MCS and GTCS latencies between the three chloroformic extract groups and the normal saline control group. No significant differences were seen in mortality rate following PTZ administration between the different extracts-treated mice and the control mice.

Conclusion:The results of the present study showed that R. damascena has an anticonvulsant effect in a mouse model of PTZ-induced seizures but the exact mechanism of this effect should be clarified in future studies.

Key words: Rosa damascena, Rosaceae, anticonvulsants, pentylenetetrazol, seizures, mice

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Group n MCS GTCS
Normal saline control 10 40.46±1.77 49.85±2.27
Aqueous extract (100 mg/kg, i.p.) 10 56.89±5.63* 77.62±5.79**
Aqueous extract (500 mg/kg, i.p.) 10 56.80±1.54* 68.80±2.73**
Aqueous extract (1 000 mg/kg, i.p.) 10 64.80±6.71** 70.11±5.39**

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Group n MCS GTCS
Normal saline control 10 40.46±1.77 49.85±2.27
Ethanolic extract (100 mg/kg, i.p.) 10 49.20±3.15 49.50±1.80
Ethanolic extract (500 mg/kg, i.p.) 10 41.70±1.64 64.66±4.48*
Ethanolic extract (1 000 mg/kg, i.p.) 10 60.60±3.14** 72.00±5.61**
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