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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 880-885.doi: 10.3736/jcim20120808

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Vasodilator effect of oroxylin A on thoracic aorta isolated from rats

Hong Wang1,2(), Jing-tian Qu1, Xin Zhao2, Ying Guo2, Hao-ping Mao1   

  1. 1. Tianjin State Key Laboratory of Modern Chinese Medicine, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    2. Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese medicine, Tianjin 300193, China
  • Received:2012-06-06 Accepted:2012-06-14 Online:2012-08-20 Published:2018-08-15
  • Contact: Wang Hong E-mail:wanghongsys@126.com

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Objective: To investigate the vasodilator effect and the endothelium-dependent mechanism of oroxylin A in thoracic aorta isolated from rats.

Methods: Thoracic aorta was isolated from Wistar rats. After pretreatment with norepinephrine or KCl, the effects of oroxylin A at different concentrations were detected on isolated vascular rings prepared from rats’ thoracic aorta. The response of thoracic aortic ring was evaluated in the presence and absence of endothelium, and N G-nitro-L-arginine methyl ester (L-NAME), a specific inhibitor of nitric oxide synthase.

Results: Oroxylin A (10 and 100 μmol/L) caused vasodilation on endothelium-intact aortic rings pretreated with norepinephrine (1 μmol/L) and KCl (60 mmol/L) compared with the control (P<0.05, P<0.01). The vasodilation function of 10 and 100 μmol/L oroxylin A on the endothelium-denuded aorta rings was significantly lower than that on the endothelium-intact aorta rings (P<0.05, P<0.01). L-NAME pretreatment significantly attenuated the effect of 100 μmol/L oroxylin A on endothelium-intact aorta rings (P<0.05, P<0.01).

Conclusion: Oroxylin A can induce the relaxation of the aorta ring in endothelium-dependent manner. Nitric oxide may be involved in the endothelium-dependent effect of oroxylin A.

Key words: plant extracts, aorta, thoracic, oroxylin A, endothelium, vascular, vasodilation, rats, in vitro

Figure 1

Structure of oroxylin A"

Figure 2

Effects of oroxylin A on contractile function of endothelium-intact aortic rings pretreated with NEData are represented as mean±standard deviation, n=6. *P<0.05, **P<0.01, vs control group. NE: norepinephrine."

Figure 3

Effects of oroxylin A on contractile function of endothelium-intact aortic rings pretreated with KClData are represented as mean±standard deviation, n=6. *P<0.05, **P<0.01, vs control group."

Figure 4

Effects of oroxylin A on contractile function of endothelium-denuded aortic rings pretreated with NEData are represented as mean±standard deviation, n=6. △P<0.05, △△P<0.01, vs +E+oroxylin A group. +E: endothelium intact rings; –E: endothelium-denuded rings; NE: norepinephrine."

Figure 5

Effects of oroxylin A on contractile function of endothelium-denuded aortic rings pretreated with KClData are represented as mean±standard deviation, n=6. △P<0.05, △△P<0.01, vs +E+oroxylin A group. +E: endothelium intact rings; –E: endothelium-denuded rings. "

Figure 6

Effects of L-NAME on the vasodilator function of oroxylin A in aorta rings pretreated with NE Data are represented as mean±standard deviation, n=6. ▲P<0.05, ▲▲P<0.01, vs oroxylin A group. L-NAME: NG-nitro-L-arginine methyl ester; NE: norepinephrine."

Figure 7

Effects of L-NAME on the vasodilator function of oroxylin A in aorta rings pretreated with KClData are represented as mean±standard deviation, n=6. ▲▲P<0.01, vs oroxylin A group. L-NAME: NG-nitro-L-arginine methyl ester."

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