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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 918-924.doi: 10.3736/jcim20120813

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Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency

Zi-hui Li1, Jian Wang1(), Rong-lin Cai2, You-wen Wang3, Jian-peng Hu4   

  1. 1. School of Clinical Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    2. Institute of Acupuncture, Moxibustion, and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    3. School of Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    4. Graduate School of Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2012-01-16 Accepted:2012-04-04 Online:2012-08-20 Published:2018-08-15
  • Contact: Jian Wang E-mail:wangjian6301@163.com

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Objective: To establish a rat model of ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy.

Methods: Sixty rats were divided into normal control group, ulcerative colitis group, ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy group (model group) and strengthening spleen for resolving dampness group. Ulcerative colitis in rats was induced by enema containing trinitrobenzene sulfonic acid (TNBS) and ethanol. The rats in the model group were suffered from standing in water, limiting sleeping time and abnormal diet based on administration of TNBS and ethanol. The rats in the spleen strengthening and dampness resolving group were gastrically administered with Shenlin Baizhu Powder, a compound traditional Chinese herbal medicine. Symptoms, signs and pathological changes in colon tissue of rats were observed after modeling. The levels of interleukin (IL)-6, IL-8 and tumor necrosis factor-α (TNF-α) in serum of rats were measured by enzyme-linked immunosorbent assay.

Results: The rats in the model group showed lethargy, poor appetite, loss of energy, diarrhea and bloody stool. Their body weight decreased significantly compared with the normal control group, and similar changes were found in the comparison of food intake, drinking amount, urine amount, stool wet weight and assay of spontaneous activity (P<0.05). When observed under a light microscope, the colon tissues of rats in the model group showed mucosal edema, congestion, inflammatory cell infiltration and ulceration. The degree of colon injury and IL-6, IL-8 and TNF-α levels were significantly increased (P<0.05) as compared to those in the normal control group. The changes mentioned above were improved by Shenlin Baizhu Powder (P<0.05).

Conclusion: The rat model of ulcerative colitis with syndrome of spleen deficiency and dampness stagnancy is successfully induced and has the characteristics of ulcerative colitis of humans both in pathological changes and in syndrome.

Key words: ulcerative colitis, syndrome of dampness stagnancy due to spleen deficiency, disease models, animal, rats

Table 1

The score criteria of colon injury degree"

Score Colon injury degree
0 No injury
1 Mucosal hyperaemia and edema, no ulcer
2 Mucosal hyperaemia, edema and mild erosion, no ulcer
3 Mucosal hyperaemia, edema and moderate erosion, with single ulcer
4 Mucosal hyperaemia, edema and severe erosion, with multiple ulcers
5 Mucosal hyperaemia, edema and severe erosion, area of the ulcers >1 cm2

Table 2

Comparison of macroscopic quantization indexes among four groups of rats （$\bar{x}$±s）"

Group n Body
weight (g)		 Food intake
in 24 h
(g/kg weight)		 Drinking amount
in 24 h
(g/kg weight)		 Urine amount
in 24 h
(g/kg weight)		 Stool wet
weight in 24 h
(g/kg weight)		 Assay of
spontaneous
activity
Normal control 15 271.8±30.2 112.3±12.8 195.7±21.3 40.5±5.9 31.8±3.7 901.3±62.7
TNBS model 15 245.4±20.3**△ 101.4±16.7*△ 147.5±18.7**△ 31.4±3.1** 50.6±6.4** 595.2±52.3**△△
Internal and
external factors
plus TNBS model		 14 208.8±21.7** 92.5±18.9** 120.5±15.6** 29.8±3.5** 58.4±6.8** 458.4±47.1**
Shenling Baizhu
Powder		 15
 258.9±27.8△△ 107.8±13.2△△ 161.7±17.5△△ 39.8±4.7△△ 33.7±4.2△△ 756.7±56.5△△

Table 3

Comparison of the score of colon injury among four groups of rats （$\bar{x}$±s）"

Group n Score of colon injury
Normal control 15 0.79±0.25
TNBS model 15 3.68±0.84**△
Internal and external factors plus TNBS model 14 4.82±0.96**
Shenling Baizhu Powder 15 1.21±0.50△△

Figure 1

Colon tissue of rats stained with hematoxylin and eosin (Light microscopy, ×200) A: Normal control group; B: Trinitrobenzene sulfonic acid group; C: Internal and external factors plus trinitrobenzene sulfonic acid group; D: Shenling Baizhu Powder group. "

Table 4

Levels of IL-6, IL-8 and TNF-α in serum of rats in different groups （$\bar{x}$±s, ng/L）"

Group n IL-6 IL-8 TNF-α
Normal control 15 27.51±2.78 34.78±3.86 0.89±0.10
TNBS model 15 37.82±5.65** 51.21±6.38** 1.69±0.15**
Internal and external factors plus TNBS model 14 39.64±4.23** 51.62±5.49** 1.87±0.19**
Shenling Baizhu Powder 15 29.01±3.28△△ 40.65±4.02*△△ 1.02±0.11△△
[1] Li HX . Observation on Shenlin Baizhu powder with herbal enema in the treatment of 47 cases of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency[J]. Liaoning Zhong Yi Za Zhi, 2008,35(6):866
李惠霞 . 参苓白术散加减配合中药灌肠治疗脾虚湿困型溃疡性结肠炎47例[J]. 辽宁中医杂志, 2008,35(6):866
[2] Yue H, Wang TF, Chen JM, Yang L, Zhao D, Zhao Y, Wu XY, Qu K, Yu LL, Zhao FC . Modern literature of common syndromes and syndrome factors of ulcerative colitis[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 2010,33(5):306-308
doi: 10.3788/HPLPB20102202.0415
岳宏, 王天芳, 陈剑明, 杨璐, 赵丹, 赵燕, 吴秀艳, 屈凯, 于丽丽, 赵绂诚 . 溃疡性结肠炎常见中医证候及证候要素的现代文献研究[J]. 北京中医药大学学报, 2010,33(5):306-308
doi: 10.3788/HPLPB20102202.0415
[3] Wang XL . Research progress on the rat models of ulcerative colitis[J]. Shanxi Zhong Yi, 2011,27(3):54-55, 60
王小莲 . 溃疡性结肠炎大鼠模型研究进展[J]. 山西中医, 2011,27(3):54-55, 60
[4] Huang XS, Shen T, Liu W, Luo YX, Wu SG, Xu W, Hou JY, Su XH, Zhang P . Effect of Pingwei powder on part of immunological function in rats with syndrome of dampness blocking spleen and stomach[J]. Zhong Yi Za Zhi, 2007,48(8):730-732
黄秀深, 沈涛, 刘伟, 罗玉熙, 吴施国, 徐薇, 侯建业, 苏先华, 张萍 . 平胃散对湿困脾胃证模型大鼠部分免疫功能的影响[J]. 中医杂志, 2007,48(8):730-732
[5] Wang XQ, Wang XY . Influences of Kuijie decoction administered by two ways on levels of serum IFN-γ and IL-4 in rats with ulcerative colitis[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 2010,33(7):468-471
王雪茜, 王新月 . 溃结饮2种给药途径对大鼠溃疡性结肠炎血清IFN-γ和IL-4的影响[J]. 北京中医药大学学报, 2010,33(7):468-471
[6] Xu SY, Bian RL, Chen X. Experimental methodology of pharmacology.3rd ed[M]. Beijing: People’s Medical Publishing House, 2002,203
徐叔云, 卞如濂, 陈修 . 药理实验方法学.第3版[M]. 北京: 人民卫生出版社, 2002,203
[7] Deng TT, Li HD. Syndrome standard of traditional Chinese medicine[M]. Guangzhou: Guangdong Science and Technology Press, 1990, 145-147
邓铁涛, 李慧德 . 中医证候规范[M]. 广州: 广东科技出版社, 1990, 145-147
[8] Wang YH. Subject on dampness disease of traditional Chinese medicine[M]. Beijing: People’s Medical Publishing House, 1997,118
王彦晖 . 中医湿病学[M]. 北京: 人民卫生出版社, 1997,118
[9] Kuang TY, Wang QQ. The pathogenesis of syndromes and signs of traditional Chinese medicine[M]. Shanghai: Shanghai Popular Science Press, 1995,89
匡调元, 王庆其 . 中医证候病理学[M]. 上海: 上海科学普及出版社, 1995,89
[10] Drug Administration of the Ministry of Health of the People’s Republic of China. Guiding principle of clinical research on spleen deficiency syndrome with Chinese medicine[J]. Zhongguo Yi Yao Xue Bao, 1988,3(5):71-72
中华人民共和国卫生部药政局. 中药治疗脾虚证的临床研究指导原则[J]. 中国医药学报, 1988,3(5):71-72
[11] Chen XY, Zhou YS, Fan YL, Zhang YP, Jiang XL, Zou SJ, Cui CD, Qiao WB, Li YM, Wang S, Wang ZY, Shao DB, Wang LF, Pang DB, Chi XS . Preliminary study on the standardization for animal models with spleen-qi deficiency syndrome[J]. Zhongguo Yi Yao Xue Bao, 2001,16(4):52-58
陈小野, 周永生, 樊雅莉, 张宇鹏, 蒋小丽, 邹世洁, 崔成德, 乔文彪, 李玉梅, 王肃, 王左原, 邵德彬, 王丽芳, 庞大本, 池旭生 . 脾气虚证动物模型规范化的初步研究[J]. 中国医药学报, 2001,16(4):52-58
[12] Zheng XB, Liu HB, Feng YL, Dai SX . Huangqin decoction lowers the number of mast cells in ulcerative colitis in rats with dampness and heat syndrome[J]. Nan Fang Yi Ke Da Xue Xue Bao, 2011,31(2):252-255
郑学宝, 刘洪波, 封艳玲, 戴世学 . 肥大细胞在湿热型溃疡性结肠炎大鼠的表达与黄芩汤的调节[J]. 南方医科大学学报, 2011,31(2):252-255
[13] Liu XP, Jia YX, Wu JJ . Effects of Tongxie Yaofang on expressions of CD44, CD54, and CD62p in rats with ulcerative colitis of liver depression and spleen deficiency syndrome[J]. Zhong Yao Yao Li Yu Lin Chuang, 2010,26(6):10-12
刘喜平, 贾育新, 吴建军 . 痛泻要方对溃疡性结肠炎肝郁脾虚证大鼠模型CD44、CD54及CD62p的影响[J]. 中药药理与临床, 2010,26(6):10-12
[14] 王忠成 . 溃疡性结肠炎的证候分布规律研究. 南京: 南京中医药大学. 2009.
Wang ZC.Research on the distribution law of traditional Chinese medicine syndromes in chronic ulcerative colitis. Nanjing: Nanjing University of Chinese Medicine. 2009. Chinese with abstract in English.
[15] Song YX.Research on the traditional Chinese medicine syndromes of ulcerative colitis. Beijing: Beijing University of Chinese Medicine. 2005. Chinese with abstract in English.
宋银雪 . 溃疡性结肠炎中医证候研究. 北京: 北京中医药大学. 2005.
[16] Duan Z, Wang WW . Research status on rat models of ulcerative colitis[J]. Chongqing Yi Xue, 2007,36(7):661-663
段征, 汪维伟 . 溃疡性结肠炎大鼠模型研究现况[J]. 重庆医学, 2007,36(7):661-663
[17] Liang HT, Song JR . Research on the correlation between TNF-α, IL-6, IL-8 levels and ulcerative colitis[J]. Zhongguo Xian Dai Yao Wu Ying Yong, 2010,4(21):92
梁皓天, 宋静荣 . TNF-α、IL-6、IL-8水平与溃疡性结肠炎相关性研究[J]. 中国现代药物应用, 2010,4(21):92
[18] Kou ST, Wu HG, Shi DR . Correlation of interleukin and ulcerative colitis[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2006,14(4):405-411
doi: 10.3969/j.issn.1009-3079.2006.04.009
口锁堂, 吴焕淦, 施达仁 . 白介素与溃疡性结肠炎[J]. 世界华人消化杂志, 2006,14(4):405-411
doi: 10.3969/j.issn.1009-3079.2006.04.009
[19] Indaram AV, Visvalingam V, Locke M, Bank S . Mucosal cytokine production in radiation-induced proctosigmoiditis compared with inflammatory bowel disease[J]. Am J Gastroenterol, 2000,95(5):1221-1225
doi: 10.1111/ajg.2000.95.issue-5
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