Search JIM Advanced Search

Journal of Integrative Medicine ›› 2014, Vol. 12 ›› Issue (6): 483-494.doi: 10.1016/S2095-4964(14)60057-1

• Research Article • Previous Articles     Next Articles

Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo

Zhang Yuan-huia,b,c,d, Wang Yuana,b,c,d, Hussein Yusufali Alic,d, Ashby Frederickc,d, Zhang Danielc,d, Yin Zi-feia,c,d, V. Aslanidi Georgec,d,e, Srivastava Arunc,d,e,f,g, Ling Chang-quana,b, Ling Chen3,4,6   

  1. Changhai Hospital of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
    Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    c Division of Cellular and Molecular Therapy, Department of Pediatrics, University of Florida College of Medicine, Gainesville, Florida 32611, USA
    Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, Florida 32611, USA
    Genetics Institute, University of Florida College of Medicine, Gainesville, Florida 32611, USA
    Shands Cancer Center, University of Florida College of Medicine, Gainesville, Florida 32611, USA
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 32611, USA
  • Received:2014-09-04 Accepted:2014-09-28 Online:2014-11-10 Published:2014-11-15


Little effort has been made to study the protein-encoding genes isolated from traditional Chinese medicine (TCM) drugs, and the delivery of these genes into malignant cells through recombinant adeno-associated virus (rAAV) vectors has not been attempted.


We synthesized the cDNAs of five known cytotoxic proteins isolated from TCM drugs and the FLAG epitope-tagged cDNAs were subcloned into a rAAV plasmid vector. The protein expression was confirmed by Western blot assay. Various cancer cell lines were transfected with the above plasmids and cell growth was monitored both in vitro and in vivo. The best cytotoxic gene was further packaged into rAAV vectors, under the control of a liver cancer-specific promoter. The liver tumor growth was then monitored following intratumor administration of the rAAV vectors. 


The expression plasmids, encoding individual potential cytotoxic genes tagged with FLAG epitope, were successfully generated and sequenced. Among these genes, trichosanthin (TCS) gene yielded the most promising results for the inhibition of cancer cell growth in vitro. The over-expressed TCS functioned as a type I ribosome-inactivating protein, followed by inducing apoptosis that is associated with the Bcl-PARP signaling pathway. Furthermore, intratumor injection of rAAV vectors containing the TCS gene significantly inhibited the growth of human hepatocellular carcinoma tumors in a murine xenograft model. 


Our studies suggest that the use of TCM cytotoxic genes is a useful therapeutic strategy for treating human cancers in general, and liver tumors in particular.

Key words: Medicine, Chinese traditional, Cytotoxic genes, Trichosanthin, Recombinant adeno-associated virus vector, Cancer gene therapy


Figure 1

Schematic outline of used plasmids AAV: adeno-associated virus; AFP: alpha-fetoprotein promoter; bGH(A)n: bovine growth hormone polyA; CBAp: chicken β-actin/cytomegalovirus enhancer hybrid promoter; CMVp: cytomegalovirus promoter; EGFP: enhanced green fluorescence protein; Gluc: Gaussia luciferase; HP: hairpin structure; GOI: gene of interest; hGH(A)n: human growth hormone polyA; HP-: hairpin structure without terminal resolution site; hrGFP: humanized recombinant green fluorescence protein; IRES: internal ribosome entry site; TCS: trichosanthin."

Figure 2

Protein translations from the potential cytotoxic traditional Chinese medicine genes A: Gel electrophoresis showing Sma I-digested and undigested plasmids; B: Fluorescence microscopy results at 48 h post-transfection with indicated plasmids in HeLa cells; C: Western blot assay showing protein expression in HeLa cells."

Figure 3

Expression of cytotoxic traditional Chinese medicine genes results in a reduction in cancer cell proliferation A: Various cancer cell types were transfected with indicated plasmids. The percentage of viable cells, compared to untransfected cells, was determined by CCK-8 kit at 48 h post-transfection. Data are expressed as mean ± standard deviation; **P<0.01, vs IRES-hrGFP. B: Various hHCC cell lines were transfected with TCS-containing plasmids. Viable cell numbers were determined by CCK-8 kit over time. The red numbers indicate the transfection efficiency in various cell lines. The experiment was repeated three times using triplicate samples in each experiment. CCK-8: Cell Counting Kit-8; hHCC: human hepatocellular carcinoma; TCS: trichosanthin."

Figure 4

Effects of intracellular expressed TCS on protein translation A: Fluorescence microscopic images at 48 h post-transfection with indicated plasmids in HeLa cells. B: Expression of TCS gene dramatically decreases Gluc expression (upper panel) at 48 h post-transfection, but not the presence of Gluc mRNA (middle panel) and DNA (lower panel). The experiment was repeated three times using triplicate samples in each experiment. TCS: trichosanthin; Gluc: Guassia luciferase."

Figure 5

Expression of TCS gene induces apoptosis A: Expression of TCS genes in HeLa cells causes an increased percentage of apoptotic cells determined by annexin V/PI double staining followed by FACS analysis. Cells treated with 1 mmol/L H2O2 or etoposide for overnight were used as positive controls. B: Western blot analysis reveals that expression of TCS gene results in changes in mitochondrial pathways in HeLa cells. C: Expression of TCS gene in different human hepatocellular carcinoma cell lines causes an increase in level of cleaved PARP. 1: Mock-treated cells; 2: Cells transfected with pAAV-CMVp-IRES-hrGFP; 3: Cells transfected with pAAV-CMVp-TCS-FLAG-IRES-hrGFP; 4: Cells treated with 1 mmol/L H2O2 for 18 h. The experiment was repeated three times using triplicate samples in each experiment. TCS: trichosanthin; PARP: poly-ADP-ribose polymerase."

Figure 6

Expression of TCS gene reduces growth of hHCC xenograft in NSG mice Huh7-Fluc cells were mock-transfected or transfected with indicated plasmid, followed by subcutaneous inoculation into NSG mice. Tumor growth was then monitored over time using in vivo bioluminescent imaging. Data are expressed as mean ± standard deviation, n=5. TCS: trichosanthin; hHCC: human hepatocellular carcinoma; Fluc: firefly luciferase."

Figure 7

Intratumor delivery of TCS gene through rAAV3 vectors significantly inhibits growth of established hHCC tumor Huh7-Fluc cells were subcutaneously injected into NSG mice for 3 weeks, followed by intratumor injection of indicated rAAV3 vectors. Tumor growth was then monitored over time using in vivo bioluminescent imaging. Data are expressed as mean ± standard deviation, n=5. TCS: trichosanthin; rAAV3: recombinant adeno-associated virus serotype 3; hHCC: human hepatocellular carcinoma."

[1] Xu J, Yang Y . Traditional Chinese medicine in the Chinese health care system[J]. Health Policy, 2009,90(2-3):133-139
doi: 10.1016/j.healthpol.2008.09.003
[2] Qiu J . China plans to modernize traditional medicine[J]. Nature, 2007,446(7136):590-591
doi: 10.1038/446590a
[3] Tang JL, Liu BY, Ma KW . Traditional Chinese medicine[J]. Lancet, 2008,372(9654):1938-1940
doi: 10.1016/S0140-6736(08)61354-9
[4] Dong S, Su SB . Advances in mesenchymal stem cells combined with traditional Chinese medicine therapy for liver fibrosis[J]. J Integr Med, 2014,12(3):147-155
doi: 10.1016/S2095-4964(14)60022-4
[5] Song HY, Zhang L, Pan JL, Yang LL, Ji G . Bioactivity of five components of Chinese herbal formula Jiangzhi granules against hepatocellular steatosis[J]. J Integr Med, 2013,11(4):262-268
doi: 10.3736/jintegrmed2013034
[6] Zhai XF, Chen Z, Li B, Shen F, Fan J, Zhou WP, Yang YK, Xu J, Qin X, Li LQ, Ling CQ . Traditional herbal medicine in preventing recurrence after resection of small hepatocellular carcinoma: a multicenter randomized controlled trial[J]. J Integr Med, 2013,11(2):90-100
doi: 10.3736/jintegrmed2013021
[7] Ling CQ, Wang LN, Wang Y, Zhang YH, Yin ZF, Wang M, Ling C . The roles of traditional Chinese medicine in gene therapy[J]. J Integr Med, 2014,12(2):67-75
doi: 10.1016/S2095-4964(14)60019-4
[8] Ng TB, Chan WY, Yeung HW . Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants[J]. Gen Pharmacol, 1992,23(4):579-590
[9] Shaw PC, Chan WL, Yeung HW, Ng TB . Minireview: trichosanthin ― a protein with multiple pharmacological properties[J]. Life Sci, 1994,55(4):253-262
doi: 10.1016/0024-3205(94)00727-6
[10] Fang EF, Ng TB, Shaw PC, Wong RN . Recent progress in medicinal investigations on trichosanthin and other ribosome inactivating proteins from the plant genus Trichosanthes[J]. Curr Med Chem, 2011,18(28):4410-4417
doi: 10.2174/092986711797200499
[11] Sha O, Niu J, Ng TB, Cho EY, Fu X, Jiang W . Anti-tumor action of trichosanthin, a type 1 ribosome-inactivating protein, employed in traditional Chinese medicine: a mini review[J]. Cancer Chemother Pharmacol, 2013,71(6):1387-1393
doi: 10.1007/s00280-013-2096-y
[12] Fang EF, Zhang CZ, Zhang L, Wong JH, Chan YS, Pan WL, Dan XL, Yin CM, Cho CH, Ng TB . Trichosanthin inhibits breast cancer cell proliferation in both cell lines and nude mice by promotion of apoptosis[J]. PLoS One, 2012,7(9):e41592
doi: 10.1371/journal.pone.0041592
[13] Shaw PC, Yung MH, Zhu RH, Ho WK, Ng TB, Yeung HW . Cloning of trichosanthin cDNA and its expression in Escherichia coli[J]. Gene, 1991,97(2):267-272
doi: 10.1016/0378-1119(91)90061-F
[14] Li B, Gu W, Zhang C, Huang XQ, Han KQ, Ling CQ . Growth arrest and apoptosis of the human hepatocellular carcinoma cell line BEL-7402 induced by melittin[J]. Onkologie, 2006,29(8-9):367-371
[15] Zhang K, Xu J, Huang X, Wu L, Wen C, Hu Y, Su Y, Chen Y, Zhang Z . Trichosanthin down-regulated p210Bcr-Abl and enhanced imatinib-induced growth arrest in chronic myelogenous leukemia cell line K562[J]. Cancer Chemother Pharmacol, 2007,60(4):581-587
doi: 10.1007/s00280-007-0457-0
[16] Lu J, Yang H, Yu H, Gao W, Lai R, Liu J, Liang X . A novel serine protease inhibitor from Bungarus fasciatus venom[J]. Peptides, 2008,29(3):369-374
doi: 10.1016/j.peptides.2007.11.013
[17] Zhao Y, Cai X, Ye T, Huo J, Liu C, Zhang S, Cao P . Analgesic-antitumor peptide inhibits proliferation and migration of SHG-44 human malignant glioma cells[J]. J Cell Biochem, 2011,112(9):2424-2434
doi: 10.1002/jcb.v112.9
[18] Liu S, Yu M, He Y, Xiao L, Wang F, Song C, Sun S, Ling C, Xu Z . Melittin prevents liver cancer cell metastasis through inhibition of the Rac1-dependent pathway[J]. Hepatology, 2008,47(6):1964-1973
doi: 10.1002/(ISSN)1527-3350
[19] Mingozzi F, High KA . Therapeutic in vivo gene transfer for genetic disease using AAV: progress and challenges[J]. Nat Rev Genet, 2011,12(5):341-355
[20] Melchiorri D, Pani L, Gasparini P, Cossu G, Ancans J, Borg JJ, Drai C, Fiedor P, Flory E, Hudson I, Leufkens HG, Müller-Berghaus J, Narayanan G, Neugebauer B, Pokrotnieks J, Robert JL, Salmonson T, Schneider CK . Regulatory evaluation of Glybera in Europe ― two committees, one mission[J]. Nat Rev Drug Discov, 2013,12(9):719
doi: 10.1038/nrd3835-c1
[21] Cheng B, Ling C, Dai Y, Lu Y, Glushakova LG, Gee SW , McGoogan KE, Aslanidi GV, Park M, Stacpoole PW, Siemann D, Liu C, Srivastava A, Ling C.Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells[J]. Gene Ther, 2012,19(4):375-384
doi: 10.1038/gt.2011.105
[22] You H, Ding W, Dang H, Jiang Y , Rountree CB.c-Met represents a potential therapeutic target for personalized treatment in hepatocellular carcinoma[J]. Hepatology, 2011,54(3):879-889
[23] Ling C, Lu Y, Kalsi JK, Jayandharan GR, Li B, Ma W, Cheng B, Gee SW , McGoogan KE, Govindasamy L, Zhong L, Agbandje-McKenna M, Srivastava A.Human hepatocyte growth factor receptor is a cellular coreceptor for adeno-associated virus serotype 3[J]. Hum Gene Ther, 2010,21(12):1741-1747
doi: 10.1089/hum.2010.075
[24] Lan T, Rao A, Haywood J, Davis CB, Han C, Garver E, Dawson PA . Interaction of macrolide antibiotics with intestinally expressed human and rat organic anion-transporting polypeptides[J]. Drug Metab Dispos, 2009,37(12):2375-2382
doi: 10.1124/dmd.109.028522
[25] Wang R, Kniazeva M, Han M . Peroxisome protein transportation affects metabolism of branched-chain fatty acids that critically impact growth and development of C. elegans[J]. PLoS One, 2013,8(9):e76270
doi: 10.1371/journal.pone.0076270
[26] Liu Y, Fang Y, Zhou Y, Zandi E, Lee CL, Joo KI, Wang P . Site-specific modification of adeno-associated viruses via a genetically engineered aldehyde tag[J]. Small, 2013,9(3):421-429
doi: 10.1002/smll.201201661
[27] Dai Y, Bae K, Siemann DW . Impact of hypoxia on the metastatic potential of human prostate cancer cells[J]. Int J Radiat Oncol Biol Phys, 2011,81(2):521-528
doi: 10.1016/j.ijrobp.2011.04.027
[28] Dai Y, Siemann DW . BMS-777607, a small-molecule met kinase inhibitor, suppresses hepatocyte growth factor-stimulated prostate cancer metastatic phenotype in vitro[J]. Mol Cancer Ther, 2010,9(6):1554-1561
doi: 10.1158/1535-7163.MCT-10-0359
[29] Gu Y, Lin S, Li JL, Nakagawa H, Chen Z, Jin B, Tian L, Ucar DA, Shen H, Lu J, Hochwald SN, Kaye FJ, Wu L . Altered LKB1/CREB-regulated transcription co-activator(CRTC) signaling axis promotes esophageal cancer cell migration and invasion[J]. Oncogene, 2011,31(4):469-479
[30] Liu B, Behura SK, Clem RJ, Schneemann A, Becnel J, Severson DW, Zhou L . P53-mediated rapid induction of apoptosis conveys resistance to viral infection in Drosophila melanogaster[J]. PLoS Pathog, 2013,9(2):e1003137
doi: 10.1371/journal.ppat.1003137
[31] Dai Y, Desano J, Qu Y, Tang W, Meng Y, Lawrence TS, Xu L . Natural IAP inhibitor Embelin enhances therapeutic efficacy of ionizing radiation in prostate cancer[J]. Am J Cancer Res, 2011,1(2):128-143
[32] Liu Y, Joo KI, Wang P . Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells[J]. Gene Ther, 2012,20(3):308-317
[33] Wang Y, Ling C, Song L, Wang L, Aslanidi GV, Tan M, Ling C, Srivastava A . Limitations of encapsidation of recombinant self-complementary adeno-associated viral genomes in different serotype capsids and their quantitation[J]. Hum Gene Ther Methods, 2012,23(4):225-233
doi: 10.1089/hgtb.2012.090
[34] Liu Y, Kim JY, Man J, Fang J, Siriwon N, Zhang L, Wang P . Enhancing gene delivery of adeno-associated viruses by cell-permeable peptides[J]. Mol Ther Methods Clin Dev, 2014,1:12
doi: 10.1038/mtm.2013.12
[35] Lin S, Tian L, Shen H, Gu Y, Li JL, Chen Z, Sun X, You MJ, Wu L . DDX5 is a positive regulator of oncogenic NOTCH1 signaling in T cell acute lymphoblastic leukemia[J]. Oncogene, 2012,32(40):4845-4853
[36] Ling CQ, Yue XQ, Ling C . Three advantages of using traditional Chinese medicine to prevent and treat tumor[J]. J Integr Med, 2014,12(4):331-335
doi: 10.1016/S2095-4964(14)60038-8
[37] Bosch MK, Nerbonne JM, Ornitz DM . Dual transgene expression in murine cerebellar purkinje neurons by viral transduction in vivo[J]. PLoS One, 2014,9(8):e104062
doi: 10.1371/journal.pone.0104062
[38] Li M, Li X, Li JC . Possible mechanisms of trichosanthin-induced apoptosis of tumor cells[J]. Anat Rec (Hoboken), 2010,293(6):986-992
doi: 10.1002/ar.21142
[39] Liu Y, Fang J, Kim YJ, Wong MK, Wang P . Codelivery of doxorubicin and paclitaxel by cross-linked multilamellar liposome enables synergistic antitumor activity[J]. Mol Pharm, 2014,11(5):1651-1661
doi: 10.1021/mp5000373
[40] Zhang Y, Zhao L, Li X, Wang Y, Yao J, Wang H, Li F, Li Z, Guo Q . V8, a newly synthetic flavonoid, induces apoptosis through ROS-mediated ER stress pathway in hepatocellular carcinoma[J]. Arch Toxicol, 2013,88(1):97-107
[1] Tara Rajendran. Addressing the need for personalizing music therapy in integrative oncology. Journal of Integrative Medicine, 2022, 20(4): 281-283.
[2] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[3] Cai-lian Fana, Wan-jun Caib, Meng-nan Ye, Miao Chen, Yi Dai. Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. Journal of Integrative Medicine, 2022, 20(4): 365-375.
[4] Shang-jin Song, Xuan Liu, Qing Ji, Da-zhi Sun, Li-juan Xiu, Jing-yu Xu, Xiao-qiang Yue. Ziyin Huatan Recipe, a Chinese herbal compound, inhibits migration and invasion of gastric cancer by upregulating RUNX3 expression. Journal of Integrative Medicine, 2022, 20(4): 355-364.
[5] Vibha Malhotra, Joanna Harnett, Erica McIntyre, Amie Steel, Keith Wong, Bandana Saini. To “tell or not to tell”—Exploring disclosure about medicine use by people living with sleep disorders. Journal of Integrative Medicine, 2022, 20(4): 338-347.
[6] Maryam Azimi, Hanieh Niayesh, Mahboobeh Raeiszadeh, Sedigheh Khodabandeh-shahraki. Efficacy of the herbal formula of Foeniculum vulgare and Rosa damascena on elderly patients with functional constipation: A double-blind randomized controlled tria. Journal of Integrative Medicine, 2022, 20(3): 230-236.
[7] Marisa Casal. Improving the health and treatment success rates of in vitro fertilization patients with traditional chinese medicine: need for more robust evidence and innovative approaches. Journal of Integrative Medicine, 2022, 20(3): 187-192.
[8] Ning Guo, Fei Wu, Mei Wu, Yuan Wang, Qing Lang, Xiao Lin, Yi Feng. Progress in the design and quality control of placeboes for clinical trials of traditional Chinese medicine . Journal of Integrative Medicine, 2022, 20(3): 204-212.
[9] Tareq A. Wani, Zahoor A. Kaloo, Nisar A. Dangroo. Aconitum heterophyllum Wall. ex Royle: A critically endangered medicinal herb with rich potential for use in medicine. Journal of Integrative Medicine, 2022, 20(2): 104-113.
[10] Mohammad Fazil, Sadia Nikhat. Why the “sugars” in traditional Unani formulations are a pivotal component: A viewpoint perspective. Journal of Integrative Medicine, 2022, 20(2): 91-95.
[11] Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[12] Deng-chao Wang, Miao Yu, Wen-xian Xie, Li-yan Huang, Jian Wei, Yue-hua Lei. Meta-analysis on the effect of combining Lianhua Qingwen with Western medicine to treat coronavirus disease 2019. Journal of Integrative Medicine, 2022, 20(1): 26-33.
[13] Mahlagha Dehghan, Alireza Ghanbari, Fatemeh Ghaedi Heidari, Parvin Mangalian, Mohammad Ali Zakerid. Use of complementary and alternative medicine in general population during COVID-19 outbreak: A survey in Iran. Journal of Integrative Medicine, 2022, 20(1): 45-51.
[14] Jose Antonio Castilla-Jimena, Isabel Ruiz-Pérez, Jesús Henares-Montiel. Impact of socioeconomic and health-related factors on consumption of homeopathic and natural remedies in Spain in 2006, 2011 and 2017. Journal of Integrative Medicine, 2022, 20(1): 52-56.
[15] Ofer Baranovitch, Meirav Wolff-Bar, Meora Feinmesser, Chen Sade-Zaltz, Ilan Tsarfaty, Victoria Neiman. Searching for the emotional roots of breast cancer: A cross-disciplinary analysis integrating psychology, Chinese medicine, and oncology biomarkers. Journal of Integrative Medicine, 2022, 20(1): 57-64.
Full text



[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022