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Journal of Integrative Medicine ›› 2016, Vol. 14 ›› Issue (1): 44-50.doi: 10.1016/S2095-4964(16)60241-8

Special Issue: Natural Drug

• Research Article • Previous Articles     Next Articles

Studies on metals and pesticide content in some Ayurvedic formulations containing Bacopa monnieri L.

AmritaMishraa,b, Arun K.Mishraa, Om PrakashTiwaria, ShiveshJhab   

  1. a Central Facility of Instrumentation, School of Pharmaceutical Sciences, IFTM University, Lodipur, Moradabad 244001, India
    b Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi 835215, India
  • Received:2015-06-05 Accepted:2015-09-28 Online:2016-01-15 Published:2016-01-15
  • Contact: Amrita Mishra; Tel: +91-9451751810; E-mail: amrita_azam@rediffmail.com

Objective

Ayurvedic formulations are preferred over other formulations as well as commercialized on broad level to treat various ailments. The World Health Organization has established certain guidelines for quality control of heavy metals and pesticide residues. Bacopa monnieri, a popular herb with immunomodulator and memory-enhancing properties is the chief constituent of several Ayurvedic formulations, which include Brahmi Vati (BV), Brahmi Ghrita (BG) and Saraswat Churna (SC), etc. In view of the World Health Organization guidelines, two products of each formulation from six different manufacturers were purchased from Ayurvedic Pharmacy, Bulanala-Varanasi, India for testing heavy metal and pesticide residue.

Methods

In the present study, all the formulations—BV, BG and SC—were selected for estimation of four heavy metals namely lead (Pb), cadmium (Cd), chromium (Cr) and nickel (Ni) by a plasma emission spectrophotometer. Organochlorine pesticidal residues were estimated for dichlorodiphenyl trichloroethane, isomers of hexachlorocyclohexane (HCH) and α-endosulfan, etc. in total 12 samples of test formulations containing Bacopa monnieri L. using gas chromatography technique.

Results

Out of 12 samples, Pb, Cd, Cr and Ni were present in all samples but below the permissible limit. Although atrazine, aldrin, dialdrin were in below detection limit, but other pesticides were detected in some samples as oxamyl, hexachlorocyclohexanes (α-HCH, β-HCH and γ-HCH), dichlorodiphenyl trichloroethane and dichlorodiphenyl dichloroethylene.

Conclusion

The presence of heavy metals in the formulations was low to cause toxicity. However evaluation of heavy metals and pesticide residue in every batch is necessary.

Key words: Ayurvedic formulations, Heavy metals, Pesticide residues, Drug contamination

1 Shankar D, Unnikrishnan PM, Venkatasubramanian P.Need to develop inter-cultural standards for quality, safety and efficacy of traditional Indian systems of medicine.Current Sci. 2007; 92(11): 1499-1505.
2 Ramar PS, Peter NP, Ponnampalam G.A compilation of bioactive compounds from Ayurveda.Bioinform. 2008; 3(3): 100-110.
3 Liang YZ, Xie P, Chan K.Quality control of herbal medicines.J Chromatogr B Analyt Technol Biomed Life Sci. 2004; 812(1-2): 53-70.
4 Anonymous. Enclclopaedia of total quality management. In: Ross JE. Fundamentals of quality management. Vol 1. New Delhi: Quality Institute of India. 2008: 27-85.
5 Mukherjee PK.Quality control of herbal drugs: an approach to evaluation of botanicals. New Delhi, India: Business Horizons. 2002: 604-608.
6 Ernst E.Heavy metals in traditional Indian remedies.Eur J Clin Pharmacol. 2002; 57(12): 891-896.
7 Yanqun Z, Yuan L, Jianjun C, Haiyan C, Li Q, Schvartz C.Hyperaccumulation of Pb, Zn and Cd in herbaceous grown on lead-zinc mining area in Yunnan, China.Environ Int. 2005; 31(5): 755-760.
8 Yoshida T, Yamauchi H, Fan Sun G.Chronic health effects in people exposed to arsenic via the drinking water: dose-response relationships in review.Toxicol Appl Pharmacol. 2004; 198(3): 243-249.
9 Chopra A, Doiphode VV. Ayurvedic medicine. Core concept, therapeutic principles,current relevance. Med Clin North Am.2002; 86(1): 75-89, vii.
10 Saper RB, Kales SN, Paquin J, Burns MJ, Eisenberg DM, Davis RB, Phillips RS.Heavy metal content of Ayurvedic herbal medicine products.JAMA. 2004; 292(23): 2868-2873.
11 Zwiebel SJ, Lee M, Alleyne B, Guyuron B.The incidence of vitamin, mineral, herbal, and other supplement use in facial cosmetic patients.Plast Reconstr Surg. 2013; 132(1): 78-82.
12 World Health Organization.WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. Geneva: World Health Organization. 2007.
13 Ghosh SP.Medicinal and aromatic plants.Ind Horticult. 1998; 43: 25-27.
14 Quality standards of Indian Medicinal Plants. New Delhi: Indian Council of Medical Research. 2005: 208.
15 Ayurvedic formulary of India, Part I & II. New Delhi: Department of AYUSH, Ministry of Health & Family Welfare, Government of India. 2005.
16 Dunbabin DW, Tallis GA, Popplewell PY, Lee RA.Lead poisoning from Indian herbal medicine (Ayurveda).Med J Aust. 1992; 157(11-12): 835-856.
17 Zuin VG, Vilegas JH.Pesticide residues in medicinal plants and phytomedicines.Phytother Res. 2000; 14(2): 73-88.
18 Corrigan FM, Wienburg CL, Shore RF, Daniel SE, Mann D.Organochlorine insecticides in substantia nigra in Parkinson’s disease.J Toxicol Environ Health A. 2000; 59(4): 229-234.
19 AOAC (Association of Official Analytical Chemists). Wet digestion for non-volatile metals. In: AOAC official methods of analysis. 16th ed. 1998: chapter 9.
20 Achliya Girish SA, Barabde U, Wadodkar S, Dorle A.Effect ofBramhi Ghrita, an polyherbal formulation on learning and memory paradigms in experimental animals. Indian J Pharmacol. 2004; 36(3): 159-162.
21 Mladenova R, Shtereva D.Pesticide residues in apples grown under a conventional and integrated pest management system.Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009; 26(6): 854-858.
22 Lal R, Pandey G, Sharma P, Kumari K, Malhotra S, Pandey R, Raina V, Kohler HP, Holliger C, Jackson C, Oakeshott JG.Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.Microbiol Mol Biol Rev. 2010; 74(1): 58-80.
23 Kannan N, Anbalagan K, Jayaraman J.Impact monitoring of pesticide residues: Rice plant (Oryzae sativa L.). Proc Indian Acad Sci (Plant Sci). 1980; 89(22): 123-130.
24 Gupta SK, Jani JP, Saiyed HN, Kashyap SK.Health hazards in pesticide formulators exposed to a combination of pesticides.Indian J Med Res. 1984; 79: 666-672.
25 Street JC.Methods of removal of pesticide residues.Can Med Assoc J. 1969; 100(4): 154-160.
26 Holland PT, Hamilton D, Ohlin B, Skidmore MW.IUPAC reports on pesticides (31). Effects of storage and processing on pesticides residues in plant products (Techical report).Pure Appl Chem. 1994; 66(2): 335-356.
27 Leung KS, Chan K, Chan CL, Lu GH.Systematic evaluation of organochlorine pesticide residues in Chinese materia medica. Phytother Res. 2005; 19(6): 514-518.
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