Cytotoxic and antiproliferative activity of kanchnar guggulu, an Ayurvedic formulation

doi:10.1016/j.joim.2018.10.001

Journal of Integrative Medicine ›› 2018, Vol. 16 ›› Issue (6): 411-417.doi: 10.1016/j.joim.2018.10.001

• Original Experimental Research • Previous Articles Next Articles

Cytotoxic and antiproliferative activity of kanchnar guggulu, an Ayurvedic formulation

Prachi Tomar ^a, Yadu Nandan Dey ^b, Deepti Sharma ^b, Manish Motiram Wanjari ^b, Sudesh Gaidhani ^c, Ankush Jadhav ^b

^a Department of Biotechnology, Kamla Raja Girls Government Postgraduate Autonomous College, Gwalior 474009, Madhya Pradesh, India
^bRegional Ayurveda Research Institute for Drug Development, Aamkho, Gwalior 474009, Madhya Pradesh, India
^c Central Council for Research in Ayurvedic Sciences, New Delhi 110058, India

Received:2018-03-09 Accepted:2018-07-31 Online:2018-11-08 Published:2018-10-04
Contact: Manish Motiram Wanjari; E-mail: manish.nriashrd@gmail.com

Abstract

Objective
Kanchnar guggulu is a compound Ayurvedic formulation used in clinical practice for the treatment of benign and malignant tumors. The present study investigates its cytotoxic and antiproliferative activities.

Methods

The hydro-alcoholic (50%) extract of kanchnar guggulu was prepared. Its antimitotic activity was assessed in an Allium cepa assay, while its antiproliferative effects were studied in a yeast proliferation model. Methotrexate was used as a standard anticancer agent.

Results

In the Allium assay, all concentrations of the extract (1, 2 and 3?mg/mL) and methotrexate (0.02?mg/mL) significantly inhibited the division of A. cepa root cells, decreasing root growth and mitotic index compared to control; this effect was concentration-dependent for kanchnar guggulu extract. In the antiproliferative studies, treatment with the hydro-alcoholic extract of kanchnar guggulu (1, 5 and 10?mg/mL) and methotrexate (0.025, 0.05 and 0.1?mg/mL) resulted in marked reduction of dividing Saccharomyces cerevisiae cells and inhibition of cell viability compared to control. The cytotoxicity of the hydro-alcoholic extract of kanchnar guggulu, shown by its antimitotic and antiproliferative effects, may be due to the presence of flavonoids and phenolics.

Conclusion

Kanchnar guggulu exhibited a cytotoxic effect by inhibiting cell division (antimitotic) and reducing cell proliferation. These results substantiate its potential for the treatment of cancer and support its traditional use in the treatment of cancer.

Key words: Allium cepa, Yeast proliferation, Antimitotic, Antiproliferative, Ayurvedic medicine

Prachi Tomar, Yadu Nandan Dey, Deepti Sharma, Manish Motiram Wanjari, Sudesh Gaidhani, Ankush Jadhav. Cytotoxic and antiproliferative activity of kanchnar guggulu, an Ayurvedic formulation. Journal of Integrative Medicine, 2018, 16(6): 411-417.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.1016/j.joim.2018.10.001

http://www.jcimjournal.com/EN/Y2018/V16/I6/411

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Cytotoxic and antiproliferative activity of kanchnar guggulu, an Ayurvedic formulation

RichHTML

PDF (PC)

Like

Abstract

Cite this article

Share this article

TrendMD

References

Related Articles 2

Metrics

Comments

Recommended 10

[1]	Shubha Singhal, Vandana Roy. Awareness, practice and views about integrating AYUSH in allopathic curriculum of allopathic doctors and interns in a tertiary care teaching hospital in New Delhi, India. Journal of Integrative Medicine, 2018, 16(2): 113-119.
[2]	Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan. Apocynaceae species with antiproliferative and/or antiplasmodial properties: A review of ten genera. Journal of Integrative Medicine, 2016, 14(4): 269-284.