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Journal of Integrative Medicine ›› 2019, Vol. 17 ›› Issue (2): 141-146.doi: 10.1016/j.joim.2019.01.004

• Original Experimental Research • Previous Articles    

Extract of Emblica officinalis enhances the growth of human keratinocytes in culture

Yoshimi Yamakami a, Kyoko Morino a, Yuki Takauji a, b, Ryuichiro Kasukabe a, Kensuke Miki a, b, Mohammad Nazir Hossain c, Dai Ayusawa a, b, Michihiko Fujii a()   

  1. a Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan
    b Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan
    c Department of Biochemistry, Primeasia University, Dhaka 1213, Bangladesh
  • Received:2018-03-15 Accepted:2018-08-18 Online:2019-03-06 Published:2019-05-18

Objective
Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here we searched for such compounds from the herbs used in traditional medicine Ayurveda.
Methods
Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2?weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography (HPLC).
Results
We found that the extract of Emblica officinalis enhanced the growth of keratinocytes in culture. Further, we fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of keratinocytes.
Conclusion
The extract of E. officinalis enhanced the growth of human keratinocytes in culture. E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing.

Key words: Keratinocyte, Cell growth, Ayurveda, Emblica officinalis

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