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Journal of Integrative Medicine ›› 2021, Vol. 19 ›› Issue (5): 451-459.doi: 10.1016/j.joim.2021.08.002

• Original Experimental Research • Previous Articles     Next Articles

The in-vivo assessment of Turkish propolis and its nano form on testicular damage induced by cisplatin

Pinar Tatli Sevena, Ismail Sevenb, Selcan Karakusc, Seda Iflazoglu Mutlua, Seyma Ozer Kayad, Gozde Arkalie, Merve Ilgarc, Ezgi Tanc, Yesim Muge Sahinf,g, Deniz Ismikf, Ayben Kilisliogluh   

  1. a. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Fırat University, Elazig 23119, Turkey 
    b. Department of Plant and Animal Production, Vocation School of Sivrice, Fırat University, Elazig 23119, Turkey
    c. Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, Istanbul 34320, Turkey 
    d. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazig 23119, Turkey
    e. Department of Physiology, Faculty of Veterinary Medicine, Fırat University, Elazig 23119, Turkey
    f. Istanbul Arel University, ArelPOTKAM (Polymer Technologies and Composite Application and Research Center), Istanbul 34537, Turkey 
    g. Department of Biomedical Engineering, Faculty of Engineering and Architecture, Istanbul Arel University, Istanbul 34537, Turkey
    h. Department of Electrical Electronics Engineering, Faculty of Engineering and Natural Sciences, Kadir Has University, Istanbul 34083, Turkey
  • Received:2021-03-29 Accepted:2021-07-03 Online:2021-09-15 Published:2021-09-30
  • Contact: Pinar Tatli Seven

Chemotherapeutic drugs, such as cisplatin (CP), which are associated with oxidative stress and apoptosis, may adversely affect the reproductive system. This study tests whether administration of propolis and nano-propolis (NP) can alleviate oxidative stress and apoptosis in rats with testicular damage induced by CP.
In this study, polymeric nanoparticles including propolis were synthesized with a green sonication method and characterized using Fourier transform-infrared spectroscopy, Brunauer-Emmett-Teller, and wet scanning transmission electron microscopy techniques. In total, 56 rats were divided into the following seven groups: control, CP, propolis, NP-10, CP + propolis, CP + NP-10, and CP + NP-30. Propolis (100 mg/kg), NP-10 (10 mg/kg), and NP-30 (30 mg/kg) treatments were administered by gavage daily for 21 d, and CP (3 mg/kg) was administered intraperitoneally in a single dose. After the experiment, oxidative stress parameters, namely, malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), and catalase (CAT), and apoptotic pathways including B cell leukemia/lymphoma-2 protein (Bcl-2) and Bcl-2-associated X protein (Bax) were measured in testicular tissues. Furthermore, sperm quality and weights of the testis, epididymis, right cauda epididymis, seminal vesicles and prostate were evaluated.
Propolis and NP (especially NP-30) were able to preserve oxidative balance (decreased MDA levels and increased GSH, CAT, and GPx activities) and activate apoptotic pathways (decreased Bax and increased Bcl-2) in the testes of CP-treated rats. Sperm motility in the control, CP, and CP + NP-30 groups were 60%, 48.75%, and 78%, respectively (P < 0.001). Especially, NP-30 application completely corrected the deterioration in sperm features induced by CP.
The results show that propolis and NP treatments mitigated the side effects of CP on spermatogenic activity, antioxidant situation, and apoptosis in rats.

Key words: Cisplatin, Nano-propolis, Reproductive system, Testicular damage

[1] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[2] Shui-jie Shen, Yong-hong Zhang, Xiao-xia Gu, Shui-ju Jiang, Ling-jun Xu. Yangfei Kongliu Formula, a compound Chinese herbal medicine, combined with cisplatin, inhibits growth of lung cancer cells through transforming growth factor-β1 signaling pathway. Journal of Integrative Medicine, 2017, 15(3): 242-251.
[3] Surendra Kr. Sharma, Naveen Goyal. Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice. Journal of Chinese Integrative Medicine, 2012, 10(5): 555-560.
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