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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 207-210.doi: 10.3736/jcim20050312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs

Pei-ting Zhu,Jing-zhe Zhang,Xue-lin Zhang,Zuo-biao Yuan,Hong-ying Zhang,Ju Gao   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

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Objective

To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis.

Methods

Sixty female guinea pigs were randomized into 3 groups: group Ⅰ (fed with normal diet), group Ⅱ (fed with low-protein diet) and group Ⅲ (fed with low-protein diet and Shengqing Capsules). After six-week feeding, the gallstone formation and the expressions of bilirubin UDP-glucuronosyltransferase (B-UGT) mRNA and cholesterol 7α-hydroxylase (CYP7A1) mRNA were observed.

Results

The proportions of stone-formed in groups Ⅰ, Ⅱ and Ⅲ were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of B-UGT and CYP7A1 mRNAs were higher in both group Ⅰ and group Ⅲ as compared with those in the group Ⅱ (P<0.05).

Conclusion

Shengqing Capsules can reduce the rate of stone-formation, which may be due to its interference of metabolism of bilirubin and cholesterol and up-regulation of the expressions of B-UGT and CYP7A1 mRNAs.

Key words: Cholelithiasis, Shengqing Capsules, B-UGT, Cholesterol 7α-hdroxylase, Gene

CLC Number: 

  • R575.62

Tab 1

Body weights of guinea pigs in 3 groups after one-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 15 264.9±35.5
Group Ⅱ (low-protein diet) 25 269.0±30.0
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 20 265.3±29.2

Tab 2

Body weights of guinea pigs in 3 groups after six-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 14 369.2±31.4*
Group Ⅱ (low-protein diet) 11 278.3±45.3
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 16 359.3±41.2*

Tab 3

Rates of stone-formation in 3 groups"

Group n Rate of stone-formed
Group Ⅰ (normal diet) 14 2/14**
Group Ⅱ (low-protein diet) 11 9/11
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 16 5/16*

Tab 4

Expressions of B-UGT and CYP7A1 mRNAs in liver of guinea pigs in 3 groups ($\bar{x}$±s, g)"

Group n B-UGT mRNA CYP7A1 mRNA
Group Ⅰ (normal diet) 8 0.48±0.10* 0.50±0.13*
Group Ⅱ （low-protein diet) 8 0.21±0.04△ 0.19±0.03△
Group Ⅲ （low-protein diet and Shengqing Capsules) 8 0.34±0.10*△ 0.34±0.09*△
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