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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 207-210.doi: 10.3736/jcim20050312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs

Pei-ting Zhu,Jing-zhe Zhang,Xue-lin Zhang,Zuo-biao Yuan,Hong-ying Zhang,Ju Gao   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

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Objective

To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis.

Methods

Sixty female guinea pigs were randomized into 3 groups: group Ⅰ (fed with normal diet), group Ⅱ (fed with low-protein diet) and group Ⅲ (fed with low-protein diet and Shengqing Capsules). After six-week feeding, the gallstone formation and the expressions of bilirubin UDP-glucuronosyltransferase (B-UGT) mRNA and cholesterol 7α-hydroxylase (CYP7A1) mRNA were observed.

Results

The proportions of stone-formed in groups Ⅰ, Ⅱ and Ⅲ were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of B-UGT and CYP7A1 mRNAs were higher in both group Ⅰ and group Ⅲ as compared with those in the group Ⅱ (P<0.05).

Conclusion

Shengqing Capsules can reduce the rate of stone-formation, which may be due to its interference of metabolism of bilirubin and cholesterol and up-regulation of the expressions of B-UGT and CYP7A1 mRNAs.

Key words: Cholelithiasis, Shengqing Capsules, B-UGT, Cholesterol 7α-hdroxylase, Gene

CLC Number: 

  • R575.62

Tab 1

Body weights of guinea pigs in 3 groups after one-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 15 264.9±35.5
Group Ⅱ (low-protein diet) 25 269.0±30.0
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 20 265.3±29.2

Tab 2

Body weights of guinea pigs in 3 groups after six-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 14 369.2±31.4*
Group Ⅱ (low-protein diet) 11 278.3±45.3
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 16 359.3±41.2*

Tab 3

Rates of stone-formation in 3 groups"

Group n Rate of stone-formed
Group Ⅰ (normal diet) 14 2/14**
Group Ⅱ (low-protein diet) 11 9/11
Group Ⅲ (low-protein diet and
Shengqing Capsules)		 16 5/16*

Tab 4

Expressions of B-UGT and CYP7A1 mRNAs in liver of guinea pigs in 3 groups ($\bar{x}$±s, g)"

Group n B-UGT mRNA CYP7A1 mRNA
Group Ⅰ (normal diet) 8 0.48±0.10* 0.50±0.13*
Group Ⅱ （low-protein diet) 8 0.21±0.04△ 0.19±0.03△
Group Ⅲ （low-protein diet and Shengqing Capsules) 8 0.34±0.10*△ 0.34±0.09*△
[1] 宋华荣, 朱培庭, 张静喆 , 等. 胆石净溶石作用的实验研究[J]. 上海中医药大学学报, 2002,16(2):42-44
[2] 徐长生, 朱培庭, 曹中平 , 等. 胆色素结石的动物模型及药物防治的实验研究[J]. 中华消化杂志, 1982,2(1):34-36
[3] Leoci C, Chiloiro M, Guerra V , et al. Genetic epidemiology of choleli thiasis: A case-control study of a population[J]. Minerua Gastroenterol Dietol, 1991,37(1):35-39
pmid: 1873328
[4] Pannwitz H, Nurnberg D, Berndt H . Epidemiology of gallbladder calculi in young females[J]. Leber Magen Darm, 1990,20(4):189-194
pmid: 2398774
[5] 杨文奇, 祝学光, 张红军 , 等. 肝脏胆红素葡萄糖醛酸化障碍对胆石形成的作用[J]. 中华消化杂志, 1999,19(1):67-68
doi: 10.3760/j.issn:0254-1432.1999.01.029
[6] 吴阶平, 裘法祖 . 黄家驷外科学(第6版)[M]. 北京: 人民卫生出版社, 2002,( 第6版):1282
[7] Shoda J, He BF, Tanaka N , et al. Primary dual defect of cholesterol and bile acid metabolism in liver of patients with intrahepatic calculi[J]. Gastroenterology, 1995,108(5):1534-1546
doi: 10.1016/0016-5085(95)90704-1
[8] Javitt NB . Choleserol homeostasis: role of the LDL receptor[J]. FASEB J, 1995,9(3):1378-1381
doi: 10.1096/fasebj.9.13.7557029 pmid: 7557029
[9] Brown MS, Goldstein JL . A receptor-mediated pathway for cholesterol homeostasis[J]. Science, 1986,232(4746):34-37
doi: 10.1126/science.3513311
[10] Vlahcevic ZR, Heuman DM, Hylemon PB . Regulation of bile acid synjournal[J]. Hepatology, 1991,13(3):590-600
doi: 10.1002/(ISSN)1527-3350
[11] Rudling M . Hepatic mRNA levels for the LDL receptor and HMG-COA reductase show coordinate regulation in vivo[J]. J Lipid Res, 1992,33(4):493-501
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