Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 207-210.doi: 10.3736/jcim20050312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs

Pei-ting Zhu,Jing-zhe Zhang,Xue-lin Zhang,Zuo-biao Yuan,Hong-ying Zhang,Ju Gao   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

Objective

To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis.

Methods

Sixty female guinea pigs were randomized into 3 groups: group Ⅰ (fed with normal diet), group Ⅱ (fed with low-protein diet) and group Ⅲ (fed with low-protein diet and Shengqing Capsules). After six-week feeding, the gallstone formation and the expressions of bilirubin UDP-glucuronosyltransferase (B-UGT) mRNA and cholesterol 7α-hydroxylase (CYP7A1) mRNA were observed.

Results

The proportions of stone-formed in groups Ⅰ, Ⅱ and Ⅲ were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of B-UGT and CYP7A1 mRNAs were higher in both group Ⅰ and group Ⅲ as compared with those in the group Ⅱ (P<0.05).

Conclusion

Shengqing Capsules can reduce the rate of stone-formation, which may be due to its interference of metabolism of bilirubin and cholesterol and up-regulation of the expressions of B-UGT and CYP7A1 mRNAs.

Key words: Cholelithiasis, Shengqing Capsules, B-UGT, Cholesterol 7α-hdroxylase, Gene

CLC Number: 

  • R575.62

Tab 1

Body weights of guinea pigs in 3 groups after one-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 15 264.9±35.5
Group Ⅱ (low-protein diet) 25 269.0±30.0
Group Ⅲ (low-protein diet and
Shengqing Capsules)
20 265.3±29.2

Tab 2

Body weights of guinea pigs in 3 groups after six-week feeding ($\bar{x}$±s, g)"

Group n Body weight
Group Ⅰ (normal diet) 14 369.2±31.4*
Group Ⅱ (low-protein diet) 11 278.3±45.3
Group Ⅲ (low-protein diet and
Shengqing Capsules)
16 359.3±41.2*

Tab 3

Rates of stone-formation in 3 groups"

Group n Rate of stone-formed
Group Ⅰ (normal diet) 14 2/14**
Group Ⅱ (low-protein diet) 11 9/11
Group Ⅲ (low-protein diet and
Shengqing Capsules)
16 5/16*

Tab 4

Expressions of B-UGT and CYP7A1 mRNAs in liver of guinea pigs in 3 groups ($\bar{x}$±s, g)"

Group n B-UGT mRNA CYP7A1 mRNA
Group Ⅰ (normal diet) 8 0.48±0.10* 0.50±0.13*
Group Ⅱ (low-protein diet) 8 0.21±0.04△ 0.19±0.03△
Group Ⅲ (low-protein diet and Shengqing Capsules) 8 0.34±0.10*△ 0.34±0.09*△
[1] 宋华荣, 朱培庭, 张静喆 , 等. 胆石净溶石作用的实验研究[J]. 上海中医药大学学报, 2002,16(2):42-44
[2] 徐长生, 朱培庭, 曹中平 , 等. 胆色素结石的动物模型及药物防治的实验研究[J]. 中华消化杂志, 1982,2(1):34-36
[3] Leoci C, Chiloiro M, Guerra V , et al. Genetic epidemiology of choleli thiasis: A case-control study of a population[J]. Minerua Gastroenterol Dietol, 1991,37(1):35-39
pmid: 1873328
[4] Pannwitz H, Nurnberg D, Berndt H . Epidemiology of gallbladder calculi in young females[J]. Leber Magen Darm, 1990,20(4):189-194
pmid: 2398774
[5] 杨文奇, 祝学光, 张红军 , 等. 肝脏胆红素葡萄糖醛酸化障碍对胆石形成的作用[J]. 中华消化杂志, 1999,19(1):67-68
doi: 10.3760/j.issn:0254-1432.1999.01.029
[6] 吴阶平, 裘法祖 . 黄家驷外科学(第6版)[M]. 北京: 人民卫生出版社, 2002,( 第6版):1282
[7] Shoda J, He BF, Tanaka N , et al. Primary dual defect of cholesterol and bile acid metabolism in liver of patients with intrahepatic calculi[J]. Gastroenterology, 1995,108(5):1534-1546
doi: 10.1016/0016-5085(95)90704-1
[8] Javitt NB . Choleserol homeostasis: role of the LDL receptor[J]. FASEB J, 1995,9(3):1378-1381
doi: 10.1096/fasebj.9.13.7557029 pmid: 7557029
[9] Brown MS, Goldstein JL . A receptor-mediated pathway for cholesterol homeostasis[J]. Science, 1986,232(4746):34-37
doi: 10.1126/science.3513311
[10] Vlahcevic ZR, Heuman DM, Hylemon PB . Regulation of bile acid synjournal[J]. Hepatology, 1991,13(3):590-600
doi: 10.1002/(ISSN)1527-3350
[11] Rudling M . Hepatic mRNA levels for the LDL receptor and HMG-COA reductase show coordinate regulation in vivo[J]. J Lipid Res, 1992,33(4):493-501
[1] Wen Qiang Lee, Jeremy Teoh, Pei Zheng Kenneth Lee, Zhi Xiong Gerard Low, Xueling Sim, Foong Fong Mary Chong, Norbert Ludwig Wagner. Factors influencing communication of traditional Chinese medicine use between patients and doctors: a multisite cross-sectional study. Journal of Integrative Medicine, 2019, 17(6): 396-403.
[2] Mohammad Ansaripour, Mohsen Naseri, Mohammad Mahdi Esfahani, Iraj Nabipour, Fatemeh Rakhshani, Arman Zargaran, Roya Kelishadi. Periconceptional care and offspring health at birth and long term, from the perspective of Avicenna. Journal of Integrative Medicine, 2019, 17(2): 80-86.
[3] Mary Abiola Okesola, Basiru Olaitan Ajiboye, Babatunji Emmanuel Oyinloye, Oluwafemi Adeleke Ojo. Neuromodulatory effects of ethyl acetate fraction of Zingiber officinale Roscoe extract in rats with lead-induced oxidative stress. Journal of Integrative Medicine, 2019, 17(2): 125-131.
[4] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
[5] Bellavite Paolo, Marzotto Marta, Bonafini Clara. Arnica montana experimental studies: Confounders and biases?. Journal of Integrative Medicine, 2018, 16(2): 72-76.
[6] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[7] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[8] Arthur Yin Fan, Sarah Faggert . Dr. Gene Bruno: The beginning of the acupuncture profession in the United States (1969-1979) — Acupuncture, medical acupuncture and animal acupuncture. Journal of Integrative Medicine, 2015, 13(5): 281-288.
[9] Oluwatosin Adaramoye, Bettina Erguen, Olubukola Oyebode, Bianca Nitzsche, Michael Höpfner, Klaus Jung, Anja Rabien. Antioxidant, antiangiogenic and antiproliferative activities of root methanol extract of Calliandra portoricensis in human prostate cancer cells. Journal of Integrative Medicine, 2015, 13(3): 185-193.
[10] Marco Gemma, Elisa Nicelli, Luigi Gioia, Elena Moizo, Luigi Beretta, Maria Rosa Calvi. Acupuncture accelerates recovery after general anesthesia: A prospective randomized controlled trial. Journal of Integrative Medicine, 2015, 13(2): 99-104.
[11] Hui-ling Wang, Nan-mei Liu, Rui Li . Role of adult resident renal progenitor cells in tubular repair after acute kidney injury. Journal of Integrative Medicine, 2014, 12(6): 469-475.
[12] Yong-hong Lian, Mei-xia Fang, Li-guo Chen. Constructing protein-protein interaction network of hypertension with blood stasis syndrome via digital gene expression sequencing and database mining. Journal of Integrative Medicine, 2014, 12(6): 476-482.
[13] Yuan-hui Zhang, Yuan Wang, Yusufali Ali Hussein, Frederick Ashby, Daniel Zhang, Zi-fei Yin, George V. Aslanidi, Arun Srivastava, Chang-quan Ling, Chen Ling. Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo. Journal of Integrative Medicine, 2014, 12(6): 483-494.
[14] Chang-quan Ling​, Xiao-qiang Yue, Chen Ling​. Three advantages of using traditional Chinese medicine to prevent and treat tumor. Journal of Integrative Medicine, 2014, 12(4): 331-335.
[15] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[10] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017