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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 211-215.doi: 10.3736/jcim20050313

• Original Experimental Research • Previous Articles     Next Articles

Impact of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in treatment of acute cholangitis in rats

Jing-zhe Zhang,Xue-lin Zhang,Ju Gao,Ping Shen,En-wei Ma,Hong-gang Gu,Pei-ting Zhu   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

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Objective

To investigate the effects of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in the treatment of acute cholangitis.

Methods

Sixty SD rats were randomly divided into group A (untreated group, acute cholangitis was induced, n=20), group B (treatment group, acute cholangitis was induced and treated with antipyretic and purgative herbs, n=20) and group C (sham operation group, n=20). At the third or fifth day after operation, the rats were sacrificed and sampled. The serum endotoxin, cytokines and inflammatory mediators were tested and the numbers of labeled bacteria in the liver, spleen and mesenteric lymph nodes translocated from the gut were assayed.

Results

As compared with group A, the serum content of endotoxin, IL-6, IL-8, TNF-α, CRP and NO was significantly lower and that of IL-2 was significantly higher, and the translocated numbers of labeled bacteria from gut were reduced in both group B and group C（P<0.01).

Conclusion

Antipyretic and purgative herbs can play therapeutic roles in the treatment of acute biliary tract infections, including the protection of intestinal mucosal barrier from bacterial translocation, reduction of serum endotoxin content and regulation of inflammatory response.

Key words: Antipyretic and purgative herbs, Cholangitis, Cytokines, Inflammatory mediators

CLC Number: 

  • R283.624

Tab 1

Serum content of cytokines in each group on the third experiment day ($\bar{x}$±s)"

Group n TNF (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 51.04±10.10** 28.47±2.98** 225.92±48.41** 922.13±192.53**
Group B 8 28.40±3.84**△△ 33.86±3.63**△△ 189.31±35.80**△ 702.11±161.42**△△
Group C 8 6.92±2.43 42.56±2.73 109.40±16.09 123.05±63.25

Tab 2

Serum content of cytokines in each group on the fifth experiment day ($\bar{x}$±s)"

Group N TNF-α (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 48.63±10.52** 25.94±4.01** 184.10±60.91** 982.80±149.56**
Group B 8 29.70±3.51**△△ 32.87±4.08**△△ 166.70±66.61** 820.10±173.91**△
Group C 8 5.76±2.20 45.76±3.45 77.40±9.06 117.10±44.64

Tab 3

Serum content of inflammatory mediators in each group on the third experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 130.10±30.85** 1 520.0±217.0**
Group B 6 98.40±16.38**△ 1 208.0±242.0**△
Group C 6 16.00±6.31 2.0±0.3

Tab 4

Serum content of inflammatory mediators in each group on the fifth experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 232.50±87.42** 1 309.0±171.0**
Group B 6 180.20±85.76** 933.0±167.0**△△
Group C 7 17.70±5.16 2.0±0.4

Tab 5

Serum content of endotoxin in each group on the third experiment day ($\bar{x}$±s, EU/ml)"

Group n Endotoxin
Group A 10 0.41±0.14
Group B 10 0.21±0.08**
Group C 10 0.08±0.03

Tab 6

Numbers of translocated bacteria in liver, spleen and mesenteric lymph nodes in each group on the third experiment day ($\bar{x}$±s, cfu/0.1 ml)"

Group n Liver Spleen MLN
Group A 10 61.60±4.45** 28.20±6.16** 36.80±8.92**
Group B 10 13.80±8.92**△△ 14.20±2.10**△△ 16.70±1.77**△△
Group C 10 2.40±1.84 2.30±2.00 4.20±2.82
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