Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 211-215.doi: 10.3736/jcim20050313

• Original Experimental Research • Previous Articles     Next Articles

Impact of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in treatment of acute cholangitis in rats

Jing-zhe Zhang,Xue-lin Zhang,Ju Gao,Ping Shen,En-wei Ma,Hong-gang Gu,Pei-ting Zhu   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

Objective

To investigate the effects of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in the treatment of acute cholangitis.

Methods

Sixty SD rats were randomly divided into group A (untreated group, acute cholangitis was induced, n=20), group B (treatment group, acute cholangitis was induced and treated with antipyretic and purgative herbs, n=20) and group C (sham operation group, n=20). At the third or fifth day after operation, the rats were sacrificed and sampled. The serum endotoxin, cytokines and inflammatory mediators were tested and the numbers of labeled bacteria in the liver, spleen and mesenteric lymph nodes translocated from the gut were assayed.

Results

As compared with group A, the serum content of endotoxin, IL-6, IL-8, TNF-α, CRP and NO was significantly lower and that of IL-2 was significantly higher, and the translocated numbers of labeled bacteria from gut were reduced in both group B and group C(P<0.01).

Conclusion

Antipyretic and purgative herbs can play therapeutic roles in the treatment of acute biliary tract infections, including the protection of intestinal mucosal barrier from bacterial translocation, reduction of serum endotoxin content and regulation of inflammatory response.

Key words: Antipyretic and purgative herbs, Cholangitis, Cytokines, Inflammatory mediators

CLC Number: 

  • R283.624

Tab 1

Serum content of cytokines in each group on the third experiment day ($\bar{x}$±s)"

Group n TNF (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 51.04±10.10** 28.47±2.98** 225.92±48.41** 922.13±192.53**
Group B 8 28.40±3.84**△△ 33.86±3.63**△△ 189.31±35.80** 702.11±161.42**△△
Group C 8 6.92±2.43 42.56±2.73 109.40±16.09 123.05±63.25

Tab 2

Serum content of cytokines in each group on the fifth experiment day ($\bar{x}$±s)"

Group N TNF-α (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 48.63±10.52** 25.94±4.01** 184.10±60.91** 982.80±149.56**
Group B 8 29.70±3.51**△△ 32.87±4.08**△△ 166.70±66.61** 820.10±173.91**
Group C 8 5.76±2.20 45.76±3.45 77.40±9.06 117.10±44.64

Tab 3

Serum content of inflammatory mediators in each group on the third experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 130.10±30.85** 1 520.0±217.0**
Group B 6 98.40±16.38** 1 208.0±242.0**
Group C 6 16.00±6.31 2.0±0.3

Tab 4

Serum content of inflammatory mediators in each group on the fifth experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 232.50±87.42** 1 309.0±171.0**
Group B 6 180.20±85.76** 933.0±167.0**△△
Group C 7 17.70±5.16 2.0±0.4

Tab 5

Serum content of endotoxin in each group on the third experiment day ($\bar{x}$±s, EU/ml)"

Group n Endotoxin
Group A 10 0.41±0.14
Group B 10 0.21±0.08**
Group C 10 0.08±0.03

Tab 6

Numbers of translocated bacteria in liver, spleen and mesenteric lymph nodes in each group on the third experiment day ($\bar{x}$±s, cfu/0.1 ml)"

Group n Liver Spleen MLN
Group A 10 61.60±4.45** 28.20±6.16** 36.80±8.92**
Group B 10 13.80±8.92**△△ 14.20±2.10**△△ 16.70±1.77**△△
Group C 10 2.40±1.84 2.30±2.00 4.20±2.82
[1] 高炬, 张静喆, 朱培庭 . 锦红片治疗急性胆道感染的临床研究[J]. 上海中医药大学学报, 2002,16(3):42-43
[2] 龚建平, 韩本立 . 急性胆道感染时肠粘膜屏障损伤的研究[J]. 中华实验外科杂志, 1991,8(3):115-116
[3] 王伟军 . 肠道细菌移位与外科临床的联系[J]. 国外医学创伤与外科基本问题分册, 1994,15(3):135-139
[4] 曹建西, 侯智亮, 彭红光 . 生大黄配合抗生素治疗急性重症胆管炎24例[J]. 中医杂志, 2004,45(2):127-128
doi: 10.3321/j.issn:1001-1668.2004.02.032
[5] 胡家石, 吴咸中 . 内镜鼻胆管引流-清解灵治疗急性重型胆管炎的临床观察[J]. 中西医结合杂志, 1989,9(3):144-147
[6] 天津市南开医院. 遵义医学院主编.新急腹症学(第1版)[M]. 北京: 人民卫生出版社, 1978,( 第1版):416-423
[7] Fong Y, Moldawer LL, Shires GT , et al. The biologic characteristics of cytokines and their implication in surgical injury[J]. Surg Gynecol Obstet, 1990,170(4):363-378
doi: 10.1002/pd.1970100414 pmid: 2108507
[8] 梁华平 . 创伤后白介素-2产生受抑的机制及其研究进展[J]. 国外医学·军事医学分册, 1991,8(4):145-149
[1] Pádraic J.Dunne, Julie Lynch, Lucia Prihodova, Caoimhe O'Leary, Atiyeh Ghoreyshi, Sharee A. Basdeo, Donal J.Cox, Rachel Breen, Ali Sheikhi, Áine Carroll, Cathal Walsh, Geraldine McMahon, Barry White. Burnout in the emergency department: Randomized controlled trial of an attention-based training program. Journal of Integrative Medicine, 2019, 17(3): 173-180.
[2] J.M. Oliver-Baxter, H.S. Whitford, D.A. Turnbull, M.J. Bond. Effects of vitamin supplementation on inflammatory markers and psychological wellbeing among distressed women: A randomized controlled trial. Journal of Integrative Medicine, 2018, 16(5): 322-328.
[3] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
[4] Lilian Uchoa Carneiro, Ilna Gomes da Silva, Mirian Eliza Alves de Souza, Wellington da Silva Côrtes, Mário Geraldo de Carvalho, Bruno Guimarães Marinho. Antinociceptive and anti-inflammatory activities of leaf extracts from Annona tomentosa R.E.Fr. Journal of Integrative Medicine, 2017, 15(5): 379-387.
[5] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[6] Wen Sun, Jian Yu, Yu-min Shi, Hao Zhang, Ying Wang, Bing-bing Wu. Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage. Journal of Chinese Integrative Medicine, 2010, 8(4): 341-346.
[7] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013.
[8] Wei Zhang, Jing-tao Cao, Hai-yu Liu. Effects of pathogenic wind-dampness on lung tissue cytokines in rats with syndrome due to pathogenic cold invading lung. Journal of Chinese Integrative Medicine, 2008, 6(7): 748-751.
[9] Sheng-li Tian, Yong-ming Zhou, Tao Huang, Zhi-zhong Xue, Wei He. Effects of Yisui Jiedu Recipe on JAK2-STAT5 signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia. Journal of Chinese Integrative Medicine, 2008, 6(2): 185-189.
[10] Jing-zhe Zhang, Xue-lin Zhang, Ju Gao, Ping Shen, Pei-ting Zhu. Impact of Jinhong Tablet on thymus of rats with cholangitis. Journal of Chinese Integrative Medicine, 2007, 5(2): 174-178.
[11] Jin-shui Zhu, Ming-quan Song, Long Wang, Qun Sun, Li Zhu, Chun Fang. Immunoregulation and short-term therapeutic effects of super-selective intra-arterial chemotherapy combined with traditional Chinese drugs on gastric cancer patients. Journal of Chinese Integrative Medicine, 2006, 4(5): 478-481.
[12] Qiang Wang​, Kun-zheng Wang​, Guang-ye Wang​. Effects of soybean isoflavones on expression levels of osteoprotegerin and osteoprotegerin ligand mRNAs in bone tissues of ovariectomized rats. Journal of Chinese Integrative Medicine, 2006, 4(3): 303-306.
[13] Jun Cai, Zheng-rong Xuan, Yong-ping Wei, Hai-bo Yang, Hua Wang. Effects of perioperative administration of rhubarb on acute inflammatory response in patients with gastric cancer. Journal of Chinese Integrative Medicine, 2005, 3(3): 195-198.
[14] Yong-qing Cao, Chun-mei He, Jin-gen Lu. Effects of Wenshen Jianpi Recipe on chronic wound healing in rats. Journal of Chinese Integrative Medicine, 2005, 3(3): 220-223.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[3] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[4] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[5] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052
[6] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[7] Liang-ping Hu, Xiao-lei Bao. Three-factor designs unable to examine the interactions (Part 2). Journal of Chinese Integrative Medicine, 2012, 10(11): 1229-1232
[8] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[9] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246
[10] Dong-tao Li, Jian Wang , Hong-yang Jiang , Feng-lei Shi , Fu-yu Li , Ji-hong Liu , Yong-mei Cheng , Nan Yan , Ai-hua Hu, Mei-zeng Zhang , Jie Li, Ling-bo Wei , Rong-qin Jiang. Quantitative evaluation of the degrees of traditional Chinese medicine qualitative syndromes of osteoporosis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1254-1262