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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (7): 748-751.doi: 10.3736/jcim20080717

• Original Experimental Research • Previous Articles     Next Articles

Effects of pathogenic wind-dampness on lung tissue cytokines in rats with syndrome due to pathogenic cold invading lung

Wei Zhang1(), Jing-tao Cao2, Hai-yu Liu2,3   

  1. 1. Department of Respiratory Diseases, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250011, China
    2. Department of Traditional Chinese Medicine,Weihai Economic and Technical Development Zone Hospital, Weihai, Shandong Province 264205, China
    3. School of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250014, China
  • Received:2007-10-17 Online:2008-07-20 Published:2008-07-15
  • Contact: ZHANG Wei

Objective: To explore the effects of wind and dampness pathogens on cytokines in the lung tissue of rats with cold syndrome due to different gradient cold pathogens.
Methods: One hundred and four Wistar rats of SPF grade were randomly divided into 13 groups: normal temperature group, six cold pathogen groups and six cold plus wind-dampness pathogen (wind of grade 5 and 90%-100% relative humidity) groups. The cold pathogens were constant low temperature (including 10 ℃, 0 ℃, –10 ℃) and temperature change (including 20 to 10 ℃, 20 to 0 ℃, and 20 to –10 ℃). The rats in different groups were kept in a temperature-controlled box under the corresponding condition for 2 hours on the first day of experiment. Then, the rats were all raised in normal temperature for 4 days and the rats' behaviors were observed. The contents of tumor necrosis factor α (TNF-α), interleukin-6(IL-6) and interleukin-4(IL-4) in lung homogenate were measured by radioimmunoassay and the content of interferon-γ (IFN-γ) was detected by enzyme-linked immunosorbent assay.
Results: In comparison with cold pathogen groups, contents of TNF-α, IL-6 and IL-4 were obviously increased in lung homogenate of rats in cold plus wind-dampness pathogen groups (P<0.01), and the content of IFN-γ and IFN-γ/IL-4 ratio were obviously decreased (P<0.01).
Conclusion: Wind-dampness pathogen can seriously aggravate the injury to lung tissue caused by cold pathogen, and the unbalance of Th1/Th2 in lung homogenate of rats.

Key words: pathogenic cold, pathogenic wind, pathogenic dampness, rats, pathogen-cold invading lung, cytokines

CLC Number: 

  • R563

Table 1

Contents of TNF-α, IL-6, IFN-γ, IL-4 and ratio of IFN-γ/IL-4 in lung tissue homogenate in different groups ($\bar{x}$±s)"

Group n TNF-α (ng/L) IL-6 (ng/L) IFN-γ (ng/L) IL-4 (ng/L) IFN-γ/IL-4
Normal temperature 8 113.00±8.41 42.54±2.08 449.63±12.61 82.51±2.58 5.44±0.19
Cold pathogen
A1 8 131.63±11.34 45.57±1.12 438.63±12.51 83.53±3.28 5.26±0.25
A2 8 201.63±16.48 52.53±3.66 419.50±24.11 90.46±3.16 4.64±0.27
A3 8 278.38±7.11 69.86±1.38 379.00±31.70 103.78±1.87 3.65±0.31
B1 8 174.00±8.94 54.09±3.37 428.13±26.22 86.68±4.75 4.94±0.27
B2 8 236.25±15.72 60.60±2.74 380.00±11.03 95.75±5.73 3.98±0.21
B3 8 321.88±39.38 79.14±4.06 206.63±11.51 113.19±9.89 1.84±0.18
Wind-cold-dampness pathogen
A1 8 186.63±13.41** 57.04±3.09** 379.63±13.18** 95.74±2.58** 3.97±0.13**
A2 8 342.38±21.56** 66.56±1.75** 347.50±34.69** 107.54±11.11** 3.26±0.48**
A3 8 411.13±9.61** 90.09±1.85** 175.25±32.12** 125.95±3.19** 1.39±0.25**
B1 8 334.13±39.03** 76.61±2.52** 359.13±9.30** 106.78±2.71** 3.39±0.13**
B2 8 408.50±17.90** 88.35±1.62** 249.50±12.64** 123.33±5.88** 2.03±0.14**
B3 7 505.57±9.73** 92.07±3.04** 145.86±9.99** 138.29±5.38** 1.06±0.10**
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