Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 10-12.doi: 10.3736/jcim20060103

• Original Clinical Research • Previous Articles     Next Articles

Relationship between female ages and the theory of five tones correlated with five zang viscera in Huangdi Neijing

Dong-li Wang1, Shan-quan Shi2, Jia He3, Sheng-bing Wu2, Xiao-xia Chen2, Hui-wei Pan2, Ye-tao Gao4   

  1. 1. Department of Traditional Chinese Medicine, Changzhou First People’s Hospital, Changzhou, Jiangsu Province 213000, China
    2. Department of Integrated Traditional Chinese and Western Medicine, Third Hospital, Second Military Medical University, Shanghai 200438, China
    3. Department of Statistics, Second Military Medical University, Shanghai 200433, China
    4. Faculty of Chinese Medicine, Macao University of Science and Technology, Macao, China
  • Online:2006-01-20 Published:2006-01-15
  • Contact: Ye-tao Gao


To find out some evidence that may indicate the theory of voice pitch of the five tones being correlated with the five zang viscera in Huangdi Neijing, which was lost 2 000 years.


Eight-four healthy females were divided into younger group (<34 years old) and older group (≥34 years old) according to their ages. The pronunciations of these females were examined by sono-frequency analysis facility.

Results: The frequency of Jiao tone was much higher than those of the other tones in both groups (P<0.01) and the frequency of Jiao tone increased as aging. The frequency of Zhi tone in the younger group was higher than that in the older group (P<0.05). 


The results of higher frequency of Jiao tone in females is in accordance with the theory put forward by Ye Gui (a famous doctor in Qing Dynasty of China) that the liver is the congenital base of female life. In traditional Chinese medicine, Jiao tone is correlated with the liver, and Zhi tone is correlated with the heart. The high frequency of Zhi tone in younger females implies that regulating the Small Intestine Meridian of Hand-Taiyang and Heart Meridian of Hand-Shaoyin may be beneficial to the health maintenance of younger female.

Key words: Huangdi Neijing, Five tones, Five zang viscera, Congenital base of life, Liver, Female

CLC Number: 

  • R229

Tab 1

Frequency distribution of five tones in two groups (Cases)"

GroupnGong tone
(262~293 Hz)
Shang tone
(294~329 Hz)
Jiao tone
(330~391 Hz)
Zhi tone
(392~439 Hz)
Yu tone
(440~523 Hz)
Younger (<34 years old)4105837102110103
Older (≥34 years old)43045471727888

Figure 1

CFemale frequency map of Jiao tone"


Female frequency map of Zhi tone"

[1] 张介宾.类经(第1版)[M].北京: 人民卫生出版社,1965.110
[2] 高也陶. 阴阳二十五人的经络调理(第1版)[M].北京: 中医古籍出版社,2003.
[3] 高也陶. 阴阳二十五人的饮食调理(第1版)[M].北京: 中医古籍出版社,2005.
[4] 高也陶, 时善全, 贺佳, 等. 《黄帝内经》五脏相音与男性年龄之关系的现代研究. 见: 中国针灸学会临床分会第三届全国代表大会暨全国针灸临床学术论坛论文集. 2005. 3-6.
[5] Pelling AE, Sehati S, Gralla EB, et al.Local nanomechanical motion of the cell wall of Saccharomyces cerevisiae[J].Science,2004,305(5687):1147-1150
doi: 10.1126/science.1097640 pmid: 15326353
[6] 韩宝强. 音的历程——现代音乐声学导论(第1版)[M].北京: 中国文联出版社,2003.306-312
[7] 颜爱心. “女子以肝为先天”的生理病理基础及临床意义[J].天津中医,2002,19(4):57
doi: 10.3969/j.issn.1672-1519.2002.04.030
[8] 王春梅. 论“女子以肝为先天”[J].湖北中医学院学报,2003,5(2):11-12
[9] 王小红. 论女子两先天说[J].福建中医药,2004,35(6):44-45
[10] 吴小明, 钟坚.肝肾共为女子先天考[J].实用中医内科杂志,2004,18(1):25-26
doi: 10.3969/j.issn.1671-7813.2004.01.013
[1] Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.
[2] Gai Ran, Xi-lin Feng, Yi-lin Xie, Qing-yun Zheng, Peng-peng Guo, Ming Yang, Ying-lu Feng, Chen Ling, Li-qing Zhu, Chen Zhong. The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy. Journal of Integrative Medicine, 2021, 19(6): 515-525.
[3] Mao-xing Pan, Chui-yang Zheng, Yuan-jun Deng, Kai-rui Tang, Huan Nie, Ji-qian Xie, Dong-dong Liu, Gui-fang Tu, Qin-he Yang, Yu-pei Zhang. Hepatic protective effects of Shenling Baizhu powder, a herbal compound, against inflammatory damage via TLR4/NLRP3 signalling pathway in rats with nonalcoholic fatty liver disease . Journal of Integrative Medicine, 2021, 19(5): 428-438.
[4] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[5] Liang Ding, Xin-you Zhang, Di-yao Wu, Meng-ling Liu. Application of an extreme learning machine network with particle swarm optimization in syndrome classification of primary liver cancer. Journal of Integrative Medicine, 2021, 19(5): 395-407.
[6] Chang-quan Ling, Jia Fan, Hong-sheng Lin, Feng Shen, Zhen-ye Xu, Li-zhu Lin, Shu-kui Qin, Wei-ping Zhou, Xiao-feng Zhai, Bai Li, Qing-hui Zhou, on behalf of the Chinese Integrative Therapy of Primary Liver Cancer Working Group. Clinical practice guidelines for the treatment of primary liver cancer with integrative traditional Chinese and Western medicine. Journal of Integrative Medicine, 2018, 16(4): 236-248.
[7] Matthias Kröz, Marcus Reif, Danilo Pranga, Roland Zerm, Friedemann Schad, Erik Wim Baars, Matthias Girke. The questionnaire on autonomic regulation: A useful concept for integrative medicine?. Journal of Integrative Medicine, 2016, 14(5): 315-321.
[8] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[9] Xuanbin Wang, Ning Wang, Fan Cheung, Lixing Lao, Charlie Li, Yibin Feng. Chinese medicines for prevention and treatment of human hepatocellular carcinoma: Current progress on pharmacological actions and mechanisms. Journal of Integrative Medicine, 2015, 13(3): 142-164.
[10] Chang-qing Zhao, Yang Zhou, Jian Ping, Lie-ming Xu. Traditional Chinese medicine for treatment of liver diseases: Progress, challenges and opportunities. Journal of Integrative Medicine, 2014, 12(5): 401-408.
[11] Jesmin Mondal, Kausik Bishayee, Ashis Kumar Panigrahi, Anisur Rahman Khuda-Bukhsh. Low doses of ethanolic extract of Boldo (Peumus boldus) can ameliorate toxicity generated by cisplatin in normal liver cells of mice in vivo and in WRL-68 cells in vitro, but not in cancer cells in vivo or in vitro. Journal of Integrative Medicine, 2014, 12(5): 425-438.
[12] Shu Dong, Shi-bing Su​. Advances in mesenchymal stem cells combined with traditional Chinese medicine therapy for liver fibrosis. Journal of Integrative Medicine, 2014, 12(3): 147-155.
[13] Cheng Huang​. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.
[14] Sarah Onyenibe Nwozo, Damilola Adeola Osunmadewa, Babatunji Emmanuel Oyinloye. Anti-fatty liver effects of oils from Zingiber officinale and Curcuma longa on ethanol-induced fatty liver in rats. Journal of Integrative Medicine, 2014, 12(1): 59-65.
[15] Sheng Wei, Jin-liang Hou, Yu-bin Chao, Xi-yang Du, Shao-bo Zong . Analysis on content of serum monoamine neurotransmitters in macaques with anger-in-induced premenstrual syndrome and liver-qi depression syndrome. Journal of Chinese Integrative Medicine, 2012, 10(8): 925-931.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278