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Journal of Integrative Medicine ›› 2014, Vol. 12 ›› Issue (1): 59-65.doi: 10.1016/S2095-4964(14)60006-6

• Research Article • Previous Articles     Next Articles

Anti-fatty liver effects of oils from Zingiber officinale and Curcuma longa on ethanol-induced fatty liver in rats

Sarah Onyenibe Nwozoa, Damilola Adeola Osunmadewaa, Babatunji Emmanuel Oyinloyea,b   

  1. a Nutritional and Industrial Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan 200002, Nigeria
    b Department of Biochemistry, College of Sciences, Afe Babalola University, Ado Ekiti 360001, Nigeria
  • Received:2013-10-31 Accepted:2013-12-20 Online:2014-01-10 Published:2014-01-15

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Objective

The present study is aimed at evaluating the protective effects of oils from Zingiber officinale (ginger) and Curcuma longa (turmeric) on acute ethanol-induced fatty liver in male Wistar rats. 

Methods

Ferric reducing antioxidant power activity and oxygen radical absorbance capacity of the oils were evaluated ex vivo. Rats were pretreated for 28 d with standard drug (Livolin Forte) and oils from Z. officinale and C. longa before they were exposed to 45% ethanol (4.8 g/kg) to induce acute fatty liver. Histological changes were observed and the degree of protection was measured by using biochemical parameters such as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities. Serum triglyceride (TG) level, total cholesterol (TC) level and the effects of both oils on reduced gluthatione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and hepatic malondialdehyde (MDA) levels were estimated. 

Results

Oils from Z. officinale and C. longa at a dose of 200 mg/kg showed hepatoprotection by decreasing the activities of serum enzymes, serum TG, serum TC and hepatic MDA, while they significantly restored the level of GSH as well as GST and SOD activities. Histological examination of rats tissues was related to the obtained results. 

Conclusion

From the results it may be concluded that oils from Z. officinale and C. longa (200 mg/kg) exhibited hepatoprotective activity in acute ethanol-induced fatty liver and Z. officinale oil was identified to have better effects than C. longa oil.

Key words: Curcuma longa, Zingiber officinale, Plant extracts, Fatty liver, In vivo

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Figure 1

Representative histological section of liver in acute toxicity (Light microscopy, ×400; haematoxylin and eosin staining) A: Group I, no visible lesions seen. B: Group II, there is marked portal congestion and cellular infiltration by mononuclear cells. C: Group III, there are periportal hydropic degeneration of hepatocytes with cellular infiltration by mononuclear cells. D: Group IV, there are mild portal congestion and mononuclear cellular infiltration, and mild hydropic degeneration. E: Group V, there are mild portal congestion, mild hydropic degeneration and few foci of cellular infiltration by mononuclear cells."

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