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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 43-47.doi: 10.3736/jcim20060112

• Original Experimental Research • Previous Articles     Next Articles

Effects of Tangshenling Mixture and benazepril on rats with diabetic nephropathy and its mechanism

Xue-lin He1, Jian-ping Li2, Yi-ping Chen3, Zhi-gang Zhang4, Wei-qin Lin1, Jiang-hua Chen1   

  1. 1. Nephrology Center, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China
    2. Department of Traditional Chinese Medicine, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China
    3. Department of Nephrology, Longhua Hospital, Shanghai University of Traditonal Chinese Medicine, Shanghai 200032, China
    4. Department of Pathology, Medical School, Fudan University, Shanghai 200032, China
  • Online:2006-01-20 Published:2006-01-15

Objective

To investigate the effects of Tangshenling Mixture (TSLM) and benazepril on rats with diabetic nephropathy (DN) and its mechanism.

Methods

Diabetic nephropathy was induced in rats by intraperitoneal injection of streptozotocin. Fifty-eight rats with DN were randomly divided into four groups: untreated group, TSLM-treated group, TSLM plus benazepril-treated group and benazepril-treated group. Another seven normal rats were included in normal control group. Then, rats in each group were accordingly given normal saline, TSLM, TSLM plus benazepril and benazepril orally for six weeks respectively. Blood and urine biochemical indexes, plasma atrial natriuretic factor (ANF), pathomorphology of renal tissue, transforming growth factor beta1 (TGF-β1) and glucose transporter 1 (GLUT1) mRNAs in renal tissue were observed.

Results

Both TSLM and benazepril could decrease urinary albumin excretion rates, creatinine clearance and ratio of kidney weight to body weight of the rats with DN as well as reduce the pathological damages of the renal tissues. TSLM could reduce the level of plasma ANF and the expression of GLUT1 mRNA, but had no significant effect on the expression of TGF-β1 mRNA. Benazepril could reduce the expression of TGF-β1 mRNA, but had no significant effect on plasma ANF and the expression of GLUT1 mRNA.

Conclusion

TSLM can reduce the pathological damages of renal tissues in rats with early-stage DN, and its mechanism may relate to decreasing the level of plasma ANF and the expression of GLUT1 mRNA which is different from that of benazepril. It seems that TSLM has synergetic effect with benazepril.

Key words: Streptozotocin diabetes, Tangshenling Mixture, Benazepril, Transforming growth factor beta1, Glucose transporter 1

CLC Number: 

  • R587.1

Tab 1

Comparison of ratio of kidney weight to body weight in five groups(ヌ±S, %)"

Group n Ratio of kidney weight
Untreated group 15 0.66±0.07△△
TSLM-treated group 14 0.39±0.04*
TSLM plus benazepril- 10 0.37±0.03**
treated group
Benazepril-treated group 15 0.41±0.05*
Normal control group 7 0.30±0.02

Tab 2

Comparison of blood glucose and HbAlc in five groups(ヌ±S)"

Group n Blood glucose HbA1c (%)
(mmol/L)
Untreated group 15 30.43±7.47** 6.61±0.97**
TSLM-treated group 14 22.64±9.04** 5.49±1.10*
TSLM plus benazepril- 10 20.97±11.35** 5.87±1.36*
treated group
Benazepril-treated group 15 26.86±9.29** 6.31±1.42*
Normal control group 7 5.65±0.71 4.29±0.28

Tab 3

Comparison of ANF, Ccr and UAlb in five groups(ヌ±S)"

Group n ANF (ng/L) Ccr (ml/min) UAlb (μg/24 h)
Untreated group 15 197.38±67.61△△ 2.46±1.94△△ 120.74±27.24△△
TSLM-treated group 14 125.44±30.29*△△ 0.92±0.60*△△ 68.17±13.33*△△
TSLM plus benazepril-treated group 10 108.66±21.68** 0.84±0.45** 60.15±12.07**△△
Benazepril-treated group 15 182.82±60.66△△ 1.49±1.15*△△ 61.14±20.48*△△
Normal control group 7 69.00±7.71 0.12±0.10 7.79±1.99

Tab 4

Comparison of TGF-β1 mRNA, GLUT1 mRNA and ratio of positive area to matrix area in five groups(ヌ±S)"

Group n TGF-β1 mRNA GLUT1 mRNA Ratio of positive area to
matrix area (%)
Untreated group 4 1.02±0.37△△ 1.04±0.24△△ 0.66±0.07△△
TSLM-treated group 4 0.89±0.37 0.79±0.16*△ 0.49±0.04*
TSLM plus benazepril-treated group 4 0.75±0.30** 0.78±0.14** 0.42±0.03**
Benazepril-treated group 4 0.78±0.26* 0.97±0.28 0.46±0.04*
Normal control group 3 0.61±0.18 0.71±0.12 0.35±0.06
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