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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (2): 189-193.doi: 10.3736/jcim20060216

• Original Experimental Research • Previous Articles     Next Articles

Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis

Chao-qin Yu1, Shu-fang Shi1, Yu-huan Liu2, Rui-xia Wang1, Yan-hua Song1, Jin Yu3   

  1. 1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    3. Gynecology and Obstetrics Hospital, Fudan University, Shanghai 200011, China
  • Online:2006-03-20 Published:2006-03-15

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Objective

To explore the method of primary culture for endometriotic cells and to find out the differences in morphological manifestations among endometriotic cells and eutopic endometrial cells sampled from patients with endometriosis and endometriosis-free women.

Methods

Endometriotic and eutopic endometrial cells were cultured by modified method of primary culture. The endometriotic cell types were observed and differentiated under optical and electron microscopes.

Results

The success rates for culture of eutopic endometrial cells from endometriosis-free women and patients with endometriosis were 91.67% and 93.75% respectively. The success rate for culture of endometriotic cells was 75.00%. The size of endometriotic glandular cells was similar to those of eutopic endometrial glandular cells from endometriosis-free women and patients with endometriosis. The chromatin was manifold and the nucleus was augmented in the endometriotic glandular cells. The endometriotic stromal cells were smaller than the eutopic endometrial stromal cells from endometriosis-free women and patients with endometriosis. Many tiny villi and protuberances on plasma membrane could be seen in the endometriotic stromal cells.

Conclusion

The success rate for culture of endometriotic cells can be elevated through improving the method of primary culture. The ultrastructures of endometriotic glandular and stromal cells are obviously different from those of eutopic endometrial glandular and stromal cells from endometriosis-free women and patients with endometriosis.

Key words: Endometriosis, Primary culture, Glandular cells, Stromal cells

CLC Number: 

  • R711.71

Figure 1

Morphological identification of human endometriotic stromal and glandular cells (SP staining, ×300)A: Endometriotic stromal cells; B: Endometriotic glandular cells."

Figure 2

Morphological characterization of glandular and stromal cells from patients with endometriosis and endometriosis-free women (HE staining, ×300)A: Eutopic endometrial glandular cells from endometriosis-free women; B: Eutopic endometrial stromal cells from endometriosis-free women; C: Eutopic endometrial glandular cells from patients with endometriosis; D: Eutopic endometrial stromal cells from patients with endometriosis; E: Endometriotic glandular cells; F: En-dometriotic stromal cells."

Figure 3

Morphological characterization of glandular and stromal cells from patients with endometriosis and endometriosis-free women by electron microscopeA: Eutopic endometrial glandular cells from endometriosis-free women (×11 000); B: Eutopic endometrial stromal cells from endometriosis-free women (×9 300); C: Eutopic endometrial glandular cells from patients with endometriosis (×11 000); D: Eutopic endometrial stromal cells from patients with endometriosis (×11 000); E: Endometriotic glandular cells (×13 000); F: Endometriotic stromal cells (×6 300)."

[1] Harada T, Kaponis A, Iwabe T , et al. Apoptosis in human endometrium and endometriosis[J]. Hum Reprod Update, 2004,10(1):29-38
[2] Collette T, Bellehumeur C, Kats R , et al. Evidence for an increased release of proteolytic activity by the eutopic endometrial tissue in women with endometriosis and for involvement of matrix metalloproteinase-9[J]. Hum Reprod, 2004,19(6):1257-1264
[3] 谭先杰, 刘东远, 郎景和 , 等. 子宫内膜腺上皮及基质细胞分离、培养作为子宫内膜异位症体外细胞模型的探索[J]. 现代妇产科进展, 2002,11(1):30-32
doi: 10.3969/j.issn.1004-7379.2002.01.010
[4] 王小敏, 黄海鹏, 何福仙 . 简易人子宫内膜细胞培养法及其优点[J]. 赣南医学院学报, 2002,22(3):256-258
doi: 10.3969/j.issn.1001-5779.2002.03.016
[5] Matthews CJ, Redfern CP, Hirst BH , et al. Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture[J]. Fertil Steril, 1992,57(5):990-997
[6] Sharpe-Timms KL, Zimmer RL, Ricke EA , et al. Endometriotic haptoglobin binds to peritoneal macrophages and alters their function in women with endometriosis[J]. Fertil Steril, 2002,78(4):810-819
[7] Dogan S, Machicao F, Wallwiener D , et al. Association of peroxisome proliferators-activated receptor gamma 2 Pro-12-Ala polymorphism with endometriosis[J]. Fertil Steril, 2004,81(5):1411-1413
[8] Koninckx PR, Barlow D, Kennedy S . Implantation versus infiltration: the Sampson versus the endometriotic disease theory[J]. Gynecol Obstet Invest, 1999,47(Supp 11):3-9
pmid: 10087422
[9] Taylor HS . Endometrial cells derived from donor stem cells in bone marrow transplant recipients[J]. JAMA, 2004,292(1):81-85
[10] Ryan IP, Schriock ED, Taylor RN . Isolation, characterization, and comparison of human endometrial and endometriosis cells in vitro[J]. J Clin Endocrinol Metab, 1994,78(3):642-649
[11] 郝敏, 石一复, 周彩云 , 等. 人子宫在位及异位内膜细胞的体外培养及生物学特性比较[J]. 生殖与避孕, 1999,19(5):301-305
[12] 江静, 吴瑞芳, 王振海 , 等. 米非司酮对离体异位与在位子宫内膜雌、孕激素受体含量的影响[J]. 中华妇产科杂志, 2001,36(4):218-221
doi: 10.7666/d.y339724
[13] Gaetje R, Kotzian S, Herrmann G , et al. Invasiveness of endometriotic cells in vitro[J]. Lancet, 1995,346(8988):1463-1464
doi: 10.1016/S0140-6736(95)92474-4 pmid: 7490993
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