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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 562-567.doi: 10.3736/jcim20100609

• Original Experimental Research • Previous Articles     Next Articles

Effects of triptolide-medicated serum on secretion function of adrenocortical cells isolated from rats

Wen-jie Maoa,Long Chena,Chun-xin Yangb,Ming-hui Yaoc,Zi-qin Zhaoa,Yi-wen Shena,Yue-qin ZhouaAi-min Xuea, Hong-mei Xua,Ming-chang Zhanga   

  1. a Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China
    b Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai 200032, China
    c Department of Pharmacology, Shanghai Medical College, Fudan University, Shanghai 200032, China
  • Received:2010-01-27 Accepted:2010-04-13 Online:2010-06-20 Published:2010-06-15
  • Contact: Long Chen E-mail:chenlong@shmu.edu.cn

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Objective

To study the effects of triptolide-medicated serum on secretory function of adrenocortical cells isolated from rats.
Methods

Thirty SD rats were randomly divided into control group, prednisone group, and low-, medium- and high-dose triptolide groups. Rats were administered with normal saline, prednisone and low-, medium- and high-dose triptolide respectively by gastrogavage to prepare sera containing drugs. Primary adrenocortical cells were isolated from normal male rats and cultured with sera containing drug for 48 hours. Expression of proliferating cell nuclear antigen (PCNA) was observed by immunohistochemical method and number of PCNA-positive cells was counted. Ultrastructure of adrenocortical cells was observed under a transmission electron microscope. Content of corticosterone in supernatant of adrenocortical cell culture was detected by enzyme-linked immunosorbent assay, and real-time fluorescence quantitative polymerase chain reaction (PCR) was employed to investigate the expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) mRNA.
Results

As compared with the control group, content of corticosterone in supernatant of adrenocortical cell culture and expression of 3β-HSD mRNA were significantly increased in the triptolide-treated groups, and the numbers of PCNA-positive cells were increased in the medium- and high-dose triptolide groups, however, they were decreased in the prednisone group.
Conclusion

Triptolide-medicated serum can increase the secretion of corticosterone in rat adrenocortical cells in vitro.

Key words: Triptolide, Chemical principles (TCD), Primary culture, Adrenocortical cells, Proliferating cell nuclear antigen, 3-hydroxysteroid dehydrogenases, Rats

Figure 1

Identification of adrenocortical cells by histochemical staining (Light microscopy, ×200)"

"

Figure 2

Ultrastructure of adrenocortical cells of rats in different groupsA: Control group (TEM, ×11 000); B: Prednisone group (TEM, ×6 500); C: Low-dose triptolide group (TEM, ×6 500); D: Medium-dose triptolide group (TEM, ×11 000); E: High-dose triptolide group (TEM, ×4 800)."

"

Figure 3

Expression of 3β-HSD mRNA in adrenocortical cells of rats in different groups**P<0.01, vs control group; △△P<0.01, vs prednisone group; ▲P<0.05, vs low-dose triptolide group."

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