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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 562-567.doi: 10.3736/jcim20100609

• Original Experimental Research • Previous Articles     Next Articles

Effects of triptolide-medicated serum on secretion function of adrenocortical cells isolated from rats

Wen-jie Maoa,Long Chena,Chun-xin Yangb,Ming-hui Yaoc,Zi-qin Zhaoa,Yi-wen Shena,Yue-qin ZhouaAi-min Xuea, Hong-mei Xua,Ming-chang Zhanga   

  1. a Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China
    b Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai 200032, China
    c Department of Pharmacology, Shanghai Medical College, Fudan University, Shanghai 200032, China
  • Received:2010-01-27 Accepted:2010-04-13 Online:2010-06-20 Published:2010-06-15
  • Contact: Long Chen E-mail:chenlong@shmu.edu.cn

Objective

To study the effects of triptolide-medicated serum on secretory function of adrenocortical cells isolated from rats.
Methods

Thirty SD rats were randomly divided into control group, prednisone group, and low-, medium- and high-dose triptolide groups. Rats were administered with normal saline, prednisone and low-, medium- and high-dose triptolide respectively by gastrogavage to prepare sera containing drugs. Primary adrenocortical cells were isolated from normal male rats and cultured with sera containing drug for 48 hours. Expression of proliferating cell nuclear antigen (PCNA) was observed by immunohistochemical method and number of PCNA-positive cells was counted. Ultrastructure of adrenocortical cells was observed under a transmission electron microscope. Content of corticosterone in supernatant of adrenocortical cell culture was detected by enzyme-linked immunosorbent assay, and real-time fluorescence quantitative polymerase chain reaction (PCR) was employed to investigate the expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) mRNA.
Results

As compared with the control group, content of corticosterone in supernatant of adrenocortical cell culture and expression of 3β-HSD mRNA were significantly increased in the triptolide-treated groups, and the numbers of PCNA-positive cells were increased in the medium- and high-dose triptolide groups, however, they were decreased in the prednisone group.
Conclusion

Triptolide-medicated serum can increase the secretion of corticosterone in rat adrenocortical cells in vitro.

Key words: Triptolide, Chemical principles (TCD), Primary culture, Adrenocortical cells, Proliferating cell nuclear antigen, 3-hydroxysteroid dehydrogenases, Rats

Figure 1

Identification of adrenocortical cells by histochemical staining (Light microscopy, ×200)"

"

Figure 2

Ultrastructure of adrenocortical cells of rats in different groupsA: Control group (TEM, ×11 000); B: Prednisone group (TEM, ×6 500); C: Low-dose triptolide group (TEM, ×6 500); D: Medium-dose triptolide group (TEM, ×11 000); E: High-dose triptolide group (TEM, ×4 800)."

"

Figure 3

Expression of 3β-HSD mRNA in adrenocortical cells of rats in different groups**P<0.01, vs control group; △△P<0.01, vs prednisone group; ▲P<0.05, vs low-dose triptolide group."

[1] Goldbach-Mansky R, Wilson M, Fleischmann R, Olsen N, Silverfield J, Kempf P, Kivitz A, Sherrer Y, Pucino F, Csako G, Costello R, Pham TH, Snyder C, van der Heijde D, Tao X, Wesley R, Lipsky PE .Comparison of Tripterygium wilfordii Hook f versus sulfasalazine in the treatment of rheumatoid arthritis: a randomized trial[J].Ann Intern Med, 2009, 151(4):229- 240, W49-W51
[2] Wang XH, Zhang ZY . Effect of tripterygium polyglucoside on T-lymphocyte subsets and serum interleukin-5 level in asthma patients[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2001,21(1):25-27
王西华, 张祖贻 . 雷公藤多甙对哮喘患者T淋巴细胞亚群和血清白细胞介素-5浓度的影响[J]. 中国中西医结合杂志, 2001,21(1):25-27
[3] Ma Z, Gao X, Zhao W, Li Y, Li C, Li C . Relationship between expression of Pad1 homologue and multidrug resistance of idiopathic nephrotic syndrome[J]. Pediatr Int, 2009,51(5):732-735
doi: 10.1111/ped.2009.51.issue-5
[4] Qin WS, Liu ZH . Mechanism of action of triptolide[J]. Shen Zang Bing Yu Tou Xi Shen Yi Zhi Za Zhi, 2007,16(2):158-161
秦卫松, 刘志红 . 雷公藤甲素及其作用机制[J]. 肾脏病与透析肾移植杂志, 2007,16(2):158-161
[5] Liu Q, Wang YQ . Research on pharmacological action of triptolide[J]. Yao Xue Jin Zhan, 2005,29(4):156-161
刘劝, 王友群 . 雷公藤甲素的药理作用研究[J]. 药学进展, 2005,29(4):156-161
[6] Carter BZ, Mak DH, Schober WD, Dietrich MF, Pinilla C, Vassilev LT, Reed JC, Andreeff M . Triptolide sensitizes AML cells to TRAIL-induced apoptosis via decrease of XIAP and p53-mediated increase of DR5[J]. Blood, 2008,111(7):3742-3750
doi: 10.1182/blood-2007-05-091504
[7] Zhang YL, He JY, Liang B, Song ZQ . Study on effects of triptolide on proliferation of airway smooth muscle cells and expression of oncogene in vitro[J]. Zhongguo Yao Xue Za Zhi, 2003,38(5):348-350
张媛莉, 何建猷, 梁标, 宋泽庆 . 雷公藤甲素对体外培养大鼠气道平滑肌细胞增殖及原癌基因的影响[J]. 中国药学杂志, 2003,38(5):348-350
[8] Ko KS, Leung WC, Ho WK, Chiu P . Herbal diterpenoids induce growth arrest and apoptosis in colon cancer cells with increased expression of the nonsteroidal anti-inflammatory drug-activated gene[J]. Eur J Pharmacol, 2007,559(1):1-13
doi: 10.1016/j.ejphar.2006.12.004
[9] Xue B, Jiao JA, Zhang L, Li KR, Gong YT, Xie JX, Wang XM . Triptolide upregulates NGF synjournal in rat astrocyte cultures[J]. Neurochem Res, 2007,32(7):1113-1119
doi: 10.1007/s11064-006-9253-1
[10] Chen L, Huang GZ, Zhang YH, Liu L, Liu SX . Experimental study of the ethylacetate extract of Tripterygium wilfordii on adrenal gland in rats[J]. Zhongguo Yao Xue Za Zhi, 1999,34(9):592-594
陈龙, 黄光照, 张益鹄, 刘良, 刘世新 . 雷公藤醋酸乙酯提取物对大鼠肾上腺影响的实验研究[J]. 中国药学杂志, 1999,34(9):592-594
[11] Chen L, Huang GZ, Li Z, Zhang YH, Liu L . The effects of Tripterygium wilfordii on nuclear DNA of adrenal glands in rats[J]. Lin Chuang Mi Niao Wai Ke Za Zhi, 1999,14(8):354-356
陈龙, 黄光照, 李中, 张益鹄, 刘良 . 雷公藤对大鼠肾上腺细胞核DNA含量的影响[J]. 临床泌尿外科杂志, 1999,14(8):354-356
[12] Chen L, Wang HJ, Zhao ZQ, Zhang Y, Huang G . Effects of the extract of a Chinese herb Tripterygium wilfordii Hook f on rat pituitary gland[J]. Am J Chin Med, 2005,33(6):945-955
doi: 10.1142/S0192415X05003521
[13] Chen L, Wang HJ, Huang GZ, Zhang YH . Effect of the ethylacetate extract of Tripterygium wilfordii on the secretion of FOS and CRH in rat hypothalamus. Proceedings of the 4th National Symposium on Tripterygium wilfordii. Shanghai. 2004: 346-348. Chinese
陈龙, 王慧君, 黄光照, 张益鹄 . 雷公藤提取物对大鼠下丘脑室旁核FOS和CRH表达的影响. 第四次全国雷公藤学术会议论文汇编. 上海. 2004: 346-348
[14] O’Hare MJ, Neville AM . Morphological responses to corticotrophin and cyclic AMP by adult rat adrenocortical cells in monolayer culture[J]. J Endocrinol, 1973,56(3):529-536
doi: 10.1677/joe.0.0560529
[15] Gao JT . Treatment of 36 corticosteroid-dependent type of aspirin asthma patients with glucosidorum Tripterygii totorum[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1994,14(12):727-729
高锦团 . 雷公藤多甙治疗激素依赖型阿斯匹林性哮喘36例[J]. 中国中西医结合杂志, 1994,14(12):727-729
[16] Li ZM, Shu DF, Liu PL, Ma ZX . Research progress of Tripterygium wilfordii Hook f[J]. Xin Zhong Yi, 1981,( 12):38-40
李志铭, 舒达夫, 刘沛霖, 马志雄 . 雷公藤研究的进展[J]. 新中医, 1981,( 12):38-40
[17] Iwama H, Amagaya S, Ogihara Y . Effect of Shaosaikoto, a Japanese and Chinese traditional herbal medicinal mixture on the mitogenic activity of lipopolysaccharide: a new pharmacological testing method[J]. J Ethnopharmacology, 1987,21(1):45-53
doi: 10.1016/0378-8741(87)90093-6
[18] Matunis MJ . On the road to repair: PCNA encounters SUMO and ubiquitin modifications[J]. Mol Cell, 2002,10(3):441-442
doi: 10.1016/S1097-2765(02)00653-6
[19] Andreis PG, Rebuffat P, Belloni AS, Neri G, Cavallini L, Gottardo G, Mazzocchi G, Coi A, Malendowicz LK, Nussdorfer GG . Stereological and functional investigations on isolated adrenocortical cells: zona fasciculata/reticularis cells of chronically ACTH-treated rats[J]. Cell Tissue Res, 1989,258(1):43-51
[20] Rainey WE, Saner K, Schimmer BP . Adrenocortical cell lines[J]. Mol Cell Endocrinol, 2004,228(1-2):23-38
doi: 10.1016/j.mce.2003.12.020
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