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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 333-337.doi: 10.3736/jcim20070320

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High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats

Li-qun He1, Jun Li2, Qi Li1   

  1. 1. Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
    2. The Fifth Hospital, Zunyi College of Medicine, Zunyi, Guizhou Province 563000, China
  • Online:2007-05-31 Published:2007-05-15

Objective: To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats.Methods: CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B2 (TXB2) and 6-keto-prostaglandin F (6-Keto-PGF) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase(SOD)were measured in blood and renal tissue of the rats.Result: HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB2, reducing the level of 6-Keto-PGF, and attenuating the activities of SOD and GSH-Px.Conclusion: HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.

Key words: kidney failure, chronic, protein, dietary, proteinuria, hemodynamics, rats

CLC Number: 

  • R692.5

Table 1

Effects of protein diet on renal function and proteinuria in CRF rats (ヌ±S)"

Group n Scr (μmol/L) BUN (mmol/L) 24 h urine pro (mg/24 h)
Normal control 7 78.90±40.42 7.93±4.35 14.32±2.04
Untreated 8 215.78±58.07**△△ 19.73±5.24△△ 151.24±30.77**△△
Middle-protein diet 7 231.90±47.79*△△ 24.10±5.28△△ 174.00±16.85**△△
High-protein diet 7 315.00±77.32△△ 25.54±6.14△△ 248.08±66.19△△
F 19.71 15.97 43.62

Table 2

Effects of protein diet on vasoactive substances in CRF rats (ヌ±S)"

Group n ET-1 (ng/L) TXB2 (ng/L) 6-Keto-PGF (ng/L)
Normal control 7 62.24±33.06 180.65±26.58 8.40±1.16
Untreated 8 135.74±33.58△△ 253.26±36.37*△ 5.12±1.55△△
Middle-protein diet 7 141.35±37.62△△ 238.07±53.21* 4.41±1.15△△
High-protein diet 7 152.22±63.78△△ 304.30±13.63△△ 4.09±1.83△△
F 6.11 11.63 11.12

Table 3

Effects of protein diet on oxidation indexes of blood in CRF rats(ヌ±S)"

Group n SOD (kU/L) MDA (μmol/L) GSH-Px (mmol/L)
Normal control 7 49.50±3.10 8.60±2.80 2.01±0.32
Untreated 8 41.30±4.60* 22.20±3.80*△△ 0.81±0.11*△△
Middle-protein diet 7 37.70±8.70△△ 24.80±5.20△△ 0.61±0.22△△
High-protein diet 6 34.10±7.50△△ 27.70±6.60△△ 0.38±0.10△△
F 7.40 21.60 81.50

Table 4

Effects of protein diet on oxidation indexes of renal tissue in CRF rats (ヌ±S)"

Group n SOD (U/ml) MDA (nmol/ml) GSH-Px (mmol/L)
Normal control 7 37.84±5.99 4.28±3.81 3.06±1.45
Untreated 8 32.67±5.53 9.68±4.77 1.57±0.56
Middle-protein diet 7 23.67±7.23*△△ 10.58±5.60 1.75±0.71
High-protein diet 6 28.90±2.70△△ 12.39±5.23△△ 1.65±0.89
F 7.64 4.56 3.55
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