SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 333-337.doi: 10.3736/jcim20070320

Previous Articles     Next Articles

High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats

Li-qun He1, Jun Li2, Qi Li1   

  1. 1. Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
    2. The Fifth Hospital, Zunyi College of Medicine, Zunyi, Guizhou Province 563000, China
  • Online:2007-05-31 Published:2007-05-15

RichHTML

2

PDF (PC)

69

Like

Objective: To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats.Methods: CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B2 (TXB2) and 6-keto-prostaglandin Flα (6-Keto-PGFlα) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase（SOD）were measured in blood and renal tissue of the rats.Result: HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB2, reducing the level of 6-Keto-PGFlα, and attenuating the activities of SOD and GSH-Px.Conclusion: HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.

Key words: kidney failure, chronic, protein, dietary, proteinuria, hemodynamics, rats

CLC Number: 

  • R692.5

Table 1

Effects of protein diet on renal function and proteinuria in CRF rats （ヌ±S）"

Group n Scr (μmol/L) BUN (mmol/L) 24 h urine pro (mg/24 h)
Normal control 7 78.90±40.42 7.93±4.35 14.32±2.04
Untreated 8 215.78±58.07**△△ 19.73±5.24△△ 151.24±30.77**△△
Middle-protein diet 7 231.90±47.79*△△ 24.10±5.28△△ 174.00±16.85**△△
High-protein diet 7 315.00±77.32△△ 25.54±6.14△△ 248.08±66.19△△
F 19.71 15.97 43.62

Table 2

Effects of protein diet on vasoactive substances in CRF rats （ヌ±S）"

Group n ET-1 (ng/L) TXB2 (ng/L) 6-Keto-PGFlα (ng/L)
Normal control 7 62.24±33.06 180.65±26.58 8.40±1.16
Untreated 8 135.74±33.58△△ 253.26±36.37*△ 5.12±1.55△△
Middle-protein diet 7 141.35±37.62△△ 238.07±53.21*△ 4.41±1.15△△
High-protein diet 7 152.22±63.78△△ 304.30±13.63△△ 4.09±1.83△△
F 6.11 11.63 11.12

Table 3

Effects of protein diet on oxidation indexes of blood in CRF rats（ヌ±S）"

Group n SOD (kU/L) MDA (μmol/L) GSH-Px (mmol/L)
Normal control 7 49.50±3.10 8.60±2.80 2.01±0.32
Untreated 8 41.30±4.60*△ 22.20±3.80*△△ 0.81±0.11*△△
Middle-protein diet 7 37.70±8.70△△ 24.80±5.20△△ 0.61±0.22△△
High-protein diet 6 34.10±7.50△△ 27.70±6.60△△ 0.38±0.10△△
F 7.40 21.60 81.50

Table 4

Effects of protein diet on oxidation indexes of renal tissue in CRF rats （ヌ±S）"

Group n SOD (U/ml) MDA (nmol/ml) GSH-Px (mmol/L)
Normal control 7 37.84±5.99 4.28±3.81 3.06±1.45
Untreated 8 32.67±5.53 9.68±4.77△ 1.57±0.56△
Middle-protein diet 7 23.67±7.23*△△ 10.58±5.60△ 1.75±0.71△
High-protein diet 6 28.90±2.70△△ 12.39±5.23△△ 1.65±0.89△
F 7.64 4.56 3.55
[1] 陈香梅, 徐启何, 王梅 , 等. 现代慢性肾衰治疗学(第1版)[M]. 北京: 人民军医出版社, 2001,( 第1版):227-227
Cheng XM, Xu QH, Wang M , et al. Modern chronic renal failure acology(1st ed)[M]. Beijing: People's Military Medical Press, 2001,( 1st ed):227-227
[2] 何立群, 郑平东, 陈刚 . 大鼠肾大部切除诱发慢性肾衰模型的建立[J]. 上海实验动物科学, 2000,20(1):11-13
He LQ, Zheng PD, Chen G . Rat model of chronic renal failure induced by subtotal nephrectomy[J]. Shanghai Shi Yan Dong Wu Ke Xue, 2000,20(1):11-13
[3] Woo KT, Lau YK . Proteinuria: clinical significance and basis for therapy[J]. Singapore Med J, 2001,42(8):385-389
pmid: 11764058
[4] Remuzzi G, Ruggenenti P, Benigni A . Understanding the nature of renal disease progression[J]. Kidney Int, 1997,51(1):2-15
[5] Perico N, Remmuzi G . Role of endothelin in glomerular injury[J]. Kidney Int (Suppl), 1993,39(1):76-80
pmid: 8468930
[6] Hocher B, Thone-Reineke C, Rohmeiss P , et al. Endothelin-1 transgenic mice develop glomerulosclerosis, interstitial fibrosis, and renal cysts but not hypertension[J]. J Clin Invest, 1997,99(6):1380-1389
[7] Naumnik B, Borawski J, Pawlak K , et al. Renal function, proteinuria and ACE-inhibitor therapy as determinants of plasma levels of endothelial markers[J]. Nephrol Dial Transplant, 2002,17(3):526-528
[8] Ruggenenti P, Gaspari F, Perna A , et al. Cross sectional longitudinal study of spot morning urine protein: creatinine ratio, 24 hour urine protein excretion rate, glomerular filtration rate, and end stage renal failure in chronic renal disease in patients without diabetes[J]. BMJ, 1998,316(7130):504-509
[9] Whinnery MA, Shaw JO, Beck N . Thromboxane B2 and prostaglandin E2 in the rat kidney with unilateral ureteral obstruction[J]. Am J Physiol, 1982,242(3):220-225
doi: 10.1152/ajprenal.1982.242.3.F220 pmid: 6950670
[10] Stahl RA, Kudelka S, Helmchen U . High protein intake stimulates glomerular prostaglandin formation in remnant kidneys[J]. Am J Physiol, 1987,252(6 Pt 2):1088-1094
doi: 10.1152/ajprenal.1987.252.6.F1088 pmid: 3473940
[11] 廖新波, 彭杰青, 熊密 , 等. 氧自由基在肾小球肾炎发病机制中的意义[J]. 广东医学院学报, 1996,14(4):307-308,317
Liao XB, Peng JQ, Xiong M , et al. The significance of oxygen free radicals in pathogenesis of antiglomerular basement membrane glomerulonephritis[J]. Guangdong Yi Xue Yuan Xue Bao, 1996,14(4):307-308, 317
[12] Nakamura K, Oka M, Shirai M , et al. Source of reactive oxygen species in anti-Thy1 nephritis[J]. Ren Fail, 1998,20(2):399-405
[13] Border WA, Okuda S, Languino LR , et al. Transforming growth factor-beta regulates production of proteoglycans by mesangial cells[J]. Kidney Int, 1990,37(2):689-695
[14] 唐政, 黎磊石 . 氧自由基及抗氧化酶与慢性肾脏疾病的进展[J]. 国外医学·泌尿系统分册, 1990,19(4):185-189
Tang Z, Li LS . Research progress of association between oxygen free radicals, antioxidase and chronic kidney diseases[J]. Guo Wai Yi Xue Mi Niao Xi Tong Fen Ce, 1990,19(4):185-189
[1] Talat Albukhari, Bassem Refaat, El-Sayed Bakr, Sameh Baz, Bodour Rajab, Hossam Gadalla, Mohamed El-Boshy. Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats. Journal of Integrative Medicine, 2022, 20(4): 348-354.
[2] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[3] Hong-xiao Li, Ling Shi, Shang-jie Liang Chen-chen Fang, Qian-qian Xu, Ge Lu, Qian Wang, Jie Cheng, Jie Shen, Mei-hong Shen. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. Journal of Integrative Medicine, 2022, 20(2): 163-172.
[4] Andréa D. Fuzimoto. An overview of the anti-SARS-CoV-2 properties of Artemisia annua, its antiviral action, protein-associated mechanisms, and repurposing for COVID-19 treatment. Journal of Integrative Medicine, 2021, 19(5): 375-388.
[5] Tong Zhao, Bing-jie Guo, Chu-lan Xiao, Jiao-jiao Chen, Can Lü, Fan-fu Fang, Bai Li. Aerobic exercise suppresses hepatocellular carcinoma by downregulating dynamin-related protein 1 through PI3K/AKT pathway. Journal of Integrative Medicine, 2021, 19(5): 418-427.
[6] Fidele Ntchapda, Fernand C. Tchatchouang, David Miaffo, Barthelemy Maidadi, Lorella Vecchio, Rodrigue E. Talla, Christian Bonabe, Paul F. Seke Etet, Theophile Dimo. Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats. Journal of Integrative Medicine, 2021, 19(4): 243-250.
[7] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[8] Pukar Khanal, Basanagouda M. Patil. Integration of network and experimental pharmacology to decipher the antidiabetic action of Duranta repens L.. Journal of Integrative Medicine, 2021, 19(1): 66-77.
[9] Johannes Loeser, Brigitte Layer, Christopher Plata, Klaus-Maria Perrar, Tim Hucho, Rebecca Kulbida. Hirudotherapy attenuates arthritic pain in patients with various chronic pain syndromes: A retrospective analysis. Journal of Integrative Medicine, 2020, 18(5): 425-433.
[10] Xian-jun Xiao, Lei-xiao Zhang, Yun-zhou Shi, Jun-peng Yao, Wei Cao, Ying Liu, Zi-hao Zou, Si-yuan Zhou, Ming-ling Chen, Chun-xiao Li, Qian-hua Zheng, Ying Li. Cupping therapy for patients with chronic urticaria: A systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(4): 303-312.
[11] Jian-sheng Li, Xue-fang Liu, Hao-ran Dong, Wan-chun Zheng, Su-xiang Feng, Yan-ge Tian, Peng Zhao, Jin-di Ma, Zhou-xin Ren, Yang Xie. Effective-constituent compatibility-based analysis of Bufei Yishen formula, a traditional herbal compound as an effective treatment for chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2020, 18(4): 351-362.
[12] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[13] Rebecca Kulbida, Alexander Mathes, Johannes Loeser. Beneficial effects of hirudotherapy in a chronic case of complex regional pain syndrome. Journal of Integrative Medicine, 2019, 17(5): 383-386.
[14] Khaled M. M. Koriem, Mahmoud S. Arbid, Hend N. Saleh . Antidiarrheal and protein conservative activities of Psidium guajava in diarrheal rats. Journal of Integrative Medicine, 2019, 17(1): 57-65.
[15] Xin-fang Zhang, Shui-ying Xiang, Wen-ye Geng, Wen-juan Cong, Jing Lu, Chuan-wei Jiang, Kun Wang, Zi-bing Liu . Electro-acupuncture regulates the cholinergic anti-inflammatory pathway in a rat model of chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2018, 16(6): 418-426.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3