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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 656-660.doi: 10.3736/jcim20070611

• Original Experimental Research • Previous Articles     Next Articles

Effect of Kangxianling Decoction on expression of hepatocyte growth factor mRNA and phosphorylations of extracellular signal-regulated protein kinase 1/2 and p38 in renal tissue of rats with unilateral ureteral obstruction

Yue Zhang1,2, Yu-min Liu1, Hai-ying Lu3, Ke-jian Liu1, Jing Li1, Li-qun He2, Min Lin1, Li Zhou1   

  1. 1. Experiment Center for Science and Technology,Shanghai University of Traditional Chinese Medicine,Shanghai 201303,China
    2. Nephropathy Research Institute,Shanghai Research Institute of Traditional Chinese Medicine,Shanghai 201303,China,3.Medicine Technique College ,Shanghai University of Traditional Chinese Medicine,Shanghai 201303,China
  • Online:2007-11-20 Published:2007-11-15

Objective: To study the effect of Kangxianling Decoction (KXLD), a compound traditional Chinese herbal medicine, on expression of hepatocyte growth factor (HGF) mRNA and phosphorylations of extracellular signal-regulated protein kinase 1/2 (ERK 1/2) and p38 in renal tissue of rats with unilateral ureteral obstruction (UUO).Methods: Eighteen male SD rats were randomly divided into 3 groups: sham-operated group, untreated group and KXLD-treated group. A rat model of renal interstitial fibrosis was established by UUO. Rats with UUO were sacrificed after intragastric administration of KXLD for 14 days, and the parameters such as serum creatinine (SCr), blood urea nitrogen (BUN) and hydroxyproline in the kidney of rats in 3 groups were analyzed. The expression of HGF mRNA in kidney tissue was determined by reverse transcription polymerase chain reaction. The expressions of c-Met protein, ERK1/2 protein, p38 protein and the phosphorylations of ERK1/2 and p38 were determined by Western blotting method.Results: The levels of SCr, BUN and hydroxyproline in the untreated group were significantly increased as compared with those in the sham-operated group (P<0.05). The expression of HGF mRNA in the untreated group was significantly down-regulated. The expression of c-Met protein and the phosphorylations of ERK1/2 and p38 in the kidney tissue of rats with UUO in the untreated group were significantly up-regulated. After intervention with KXLD, the phosphorylations of ERK1/2 and p38 were all significantly inhibited except for c-Met expression. The HGF mRNA was increased in KXLD-treated group.Conclusion: KXLD can decrease the level of collagen in the obstructed kidney of rats with UUO and alleviate the renal interstitial fibrosis in rats with UUO through enhancing the HGF mRNA expression and inhibiting the phosphorylations of ERK1/2 and p38.

Key words: ureteral obstruction, mitogen-activated protein kinase-extracellular signal-regulated protein kinase, hepatocyte growth factor, proto-oncogene protein c-Met

CLC Number: 

  • R693

Table 1

Sequence, melting temperature of primer and product length"

Primer Sequence (5’-3’) Melting temperature (℃) Product length (bp)
Hepatocyte growth factor Forward: GAGGAGAAACGCAAACAG 56 414
Reverse: ACGACCAGGAACAATGAC
β-actin Forward: TGACGAGGCCCAGAGCAAGA 56/60 330
Rerverse: ATGGGCACAGTGTGGGTGAC

Table 2

Content of BUN, Scr and hydroxyproline in the obstructed kidney of rats with UUO in different group (ヌ±S)"

Group n BUN (μmol/L) Scr (μmol/L) Hydroxyproline (μg/g)
Sham-operated 6 6.08±0.99 21.5±3.73 0.49±0.08
Untreated 6 18.34±2.00* 32.0±2.55* 1.004±0.28*
KXLD-treated 6 8.42±1.69* 30.5±4.43* 0.768±0.06*

Figure 1

Expression of HGF mRNA in kidney tissue of rats with UUO in different groups"

Figure 2

Effect of KXLD on the enhanced c-Met protein expression in rats with UUO"

Table 3

Antagonism of KXLD on ERK1/2 and p38 phosphorylations in rats with UUO (ヌ±S)"

Group n P-ERK/ERK ERK/GAPDH P-p38/p38 p38/GAPDH
Sham-operated 4 0.069±0.058 0.942±0.037 0.819±0.214 1.597±0.045
Untreated 4 2.250±0.487* 1.137±0.184 1.44±0.151* 1.605±0.136
KXLD-treated 4 0.431±0.337△ 1.076±0.095 0.846±0.295△ 1.241±0.132

Figure 3

Antagonism of KXLD on ERK1/2 protein phosphorylation in rats with UUO"

Figure 4

Antagonism of KXLD on p38 proteinphosphorylation in rats with UUO"

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