复方鳖甲软肝片对阿霉素肾病大鼠肾组织结缔组织生长因子mRNA及蛋白表达的影响

doi:10.3736/jcim200706010

Abstract

Objective: To investigate the changes of connective tissue growth factor (CTGF) protein and CTGF mRNA in kidney tissue of rats with adriamycin (ADR)-induced nephropathy and to study the effects of compound Biejia Ruangan tablet (CBJRGT), a traditional Chinese herbal medicine for treatment of liver fibrosis.Methods: A rat model of ADR-induced nephropathy after one-sided nephrectomy was established. Forty-five Wistar rats were randomly divided into 5 groups: normal control group, sham-operated group, untreated group, lotensin-treated group and CBJRGT-treated group. Pathological changes of the kidney tissue were observed by microscopy after 10-week drug administration. The expressions of CTGF protein and CTGF mRNA in the kidney tissue were measured by the methods of immunohistochemistry and in situ hybridization.Results: The expressions of CTGF protein and CTGF mRNA in the normal and sham-operated groups were decreased in the intracytoplasm of glomerular mesangial cells, renal tubular epithelial cells and interstitial cells. Compared with the sham-operated group, the expressions of CTGF protein and CTGF mRNA in the untreated group were markedly increased and the development of renal fibrosis in the untreated group could be observed. CBJRGT could significantly decrease the expressions of CTGF protein and CTGF mRNA, and there was no significant difference between CBJRGT-treated group and lotensin-treated group.Conclusion: CBJRGT may suppress the development of fibrosis through down-regulating the expressions of CTGF protein and CTGF mRNA.

Key words: connective tissue growth factor, nephrosis, carpax trionycis, rats

CLC Number:

R692

Zong-jiang Zhao, Qi Qiu, Xin-xue Zhang, Mei-juan Yang. Effect of compound Biejia Ruangan tablet on expressions of connective tissue growth factor mRNA and protein in kidney tissue of rats with adriamycin-induced nephropathy. Journal of Chinese Integrative Medicine, 2007, 5(6): 651-655.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim200706010

http://www.jcimjournal.com/EN/Y2007/V5/I6/651

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References 12

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Group	n	2 weeks	4 weeks	6 weeks	8 weeks	10 weeks
Normal control	9	8.66±3.11	11.00±3.20	12.20±4.29	10.30±3.12	12.20±3.53
Untreated	9	27.03±10.82**▲▲	36.97±15.90**▲▲	49.52±11.95**▲▲	64.55±15.25**▲▲	71.91±13.93**▲▲
Sham-operated	9	17.30±5.33	15.20±4.82	12.40±2.27	14.10±3.66	20.60±5.90
Lotensin-treated	9	26.88±10.08**	30.14±16.00**	42.10±12.80**	42.26±12.89**^△△	54.08±22.71**^△
CBJRGT-treated	9	17.62±7.38△□	35.94±9.65**	28.80±18.94**^△△	57.86±15.87**	63.06±9.59**

Group	n	BUN (mmol/L)	Scr (μmol/L)	CHO (mmol/L)	TG (mmol/L)	TP (mmol/L)	ALB (mmol/L)
Normal control	9	5.95±0.27	44.27±2.61	1.49±0.17	0.86±0.46	39.97±9.04	24.80±4.50
Untreated	9	10.89±2.48*	59.04±7.17*	8.06±0.83*	3.50±3.02*	29.34±14.64*	15.36±4.60*
Sham-operated	9	6.83±1.66	47.25±7.75	1.96±0.58	1.57±1.56	29.25±5.96	18.10±2.54
Lotensin-treated	9	6.11±2.50^△	49.70±6.80^△	3.67±1.19^△	2.45±1.87^△	29.64±7.63	18.31±2.12^△
CBJRGT-treated	9	6.19±1.80^△	44.66±6.62^△	2.17±1.70^△	2.67±1.70^△	29.39±5.88	17.88±3.00^△

Group	n	CTGF protein	CTGF mRNA
Normal control	5	3.446±1.157	3.604±1.359
Untreated	5	21.364±4.113**	12.760±3.137**
Sham-operated	5	4.612±1.695	4.060±1.931
Lotensin-treated	5	6.998±1.461^△△	6.656±1.256^△△
CBJRGT-treated	5	9.186±2.392^△△	8.554±2.152^△

Effect of compound Biejia Ruangan tablet on expressions of connective tissue growth factor mRNA and protein in kidney tissue of rats with adriamycin-induced nephropathy

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