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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 670-674.doi: 10.3736/jcim20070614

• Original Experimental Research • Previous Articles     Next Articles

Effect of Liriope platyphylla total saponin on learning, memory and metabolites in aging mice induced by D-galactose

Tao Jiang1,2, Bao-kang Huang1, Qiao-yan Zhang1, Ting Han1, Han-cheng Zheng1, Lu-ping Qin1   

  1. 1. Department of Pharmacognosy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
    2. Shanghai Nabio Biotechnology Co,Ltd,Shanghai 201203, China
  • Online:2007-11-20 Published:2007-11-15

Objective: To investigate the effects of Liriope platyphylla total saponin (LPTS) on learning, memory, neuromediators and metabolites in aging mice induced by D-galactose.Methods: Ninety Kunming mice were randomly divided into nine groups: normal saline (NS)-treated group, untreated group, high- (100 mg/kg), medium- (50 mg/kg) and low-dose (10 mg/kg) LPTS-treated groups, Shuxuening-treated group, jiaogulanosidi-treated group, flunarizine-treated group and vitamin E-treated group. The Kunming mice in the NS-treated group were administered with NS by intraperitoneal injection, while the aging mice in the other eight groups were administered with D-galactose by intraperitoneal injection. At the same time, the aging mice in different groups were fed with corresponding drugs for 42 days, and the aging items of the mice in different groups were measured, respectively.Results: LPTS could improve the memory of aging mice induced by D-galactose, promote its body weight, and increase the thymus and spleen indexes of the aging mice. LPTS could decrease the levels of MDA and lipofuscin, inhibit MAO activity and increase SOD activity and GSH-Px level.Conclusion: LPTS may improve the ability of learning and memory and delay aging.

Key words: Liriope platyphylla, saponins, galactose, aging, memory disorders, mic

CLC Number: 

  • R339.38

Table 1

Effect of LPTS on latent period of aging mice induced by D-galactase ($\bar{x}±S$,s)"

Group n Day 1 Day 2 Day 3 Day 4 Day 5
NS-treated 10 60.0±0.0 42.5±14.3 36.9±21.8 37.3±16.7 32.6±10.8
Untreated 10 60.0±0.0 52.7±15.1 48.6±10.7 47.3±18.0 41.6±17.9
Low-dose LPTS-treated 10 60.0±0.0 50.8±10.1 47.6±15.0 47.3±15.0 38.3±21.0
Medium-dose LPTS-treated 10 60.0±0.0 46.6±18.6 45.5±14.5 42.8±19.5 36.2±11.6
High-dose LPTS-treated 10 60.0±0.0 46.5±18.4 43.8±12.6 35.9±15.2 33.9±15.3**
Shuxuening-treated 10 60.0±0.0 46.3±14.2 42.3±18.3 42.8±19.5 30.0±16.5**

Table 2

Effect of LPTS on memory frequency of aging mice induced by D-galactase ($\bar{x}±S$,%)"

Group n Memory frequency
NS-treated 10 17.6±4.6
Untreated 10 14.1±7.3
Low-dose LPTS-treated 10 13.2±4.7
Medium-dose LPTS-treated 10 13.9±7.7
High-dose LPTS-treated 10 21.5±4.6*
Shuxuening-treated 10 13.6±4.2

Table 3

Effect of LPTS on memory score rate of aging mice induced by D-galactase ($\bar{x}±S$,%)"

Group n Memory score rate
NS-treated 10 10.0±5.0
Untreated 10 8.4±4.4
Low-dose LPTS-treated 10 6.8±5.7
Medium-dose LPTS-treated 10 8.0±6.5
High-dose LPTS-treated 10 7.2±2.6
Shuxuening-treated 10 6.6±4.9*

Table 4

Effect of LPTS on body weight of aging mice induced by D-galactase ($\bar{x}±S$, g)"

Table 5

Effect of LPTS on indexes of related organs in aging mice induced by D-galactase ($\bar{x}±S$, mg/g)"

Group n Organ index
Brain Liver Kidney Thymus Spleen
NS-treated 10 11.8±2.3 53.4±14.2 12.8±3.0 2.8±0.9 4.0±1.4
Untreated 10 12.6±1.9 50.9±7.5 12.1±1.8 1.4±1.2 3.3±0.5
Low-dose LPTS-treated 10 11.9±1.2 43.6±3.4 12.0±2.2 2.2±2.4 3.5±2.2
Medium-dose LPTS-treated 10 12.0±2.0 46.8±3.0 12.7±3.0 2.4±0.4* 4.1±1.0*
High-dose LPTS-treated 10 12.2±1.8 48.2±2.5 13.8±1.8 2.4±0.7* 4.5±0.8**
Shuxuening-treated 10 11.3±2.0 53.6±3.4 13.9±2.8 2.8±0.8** 4.3±0.8*
Jiaogulanosidi-treated 10 11.3±1.3 46.0±4.0 12.3±0.9 2.3±0.3 4.9±1.8*
VE-treated 10 10.6±1.5 44.4±17.6 13.8±1.5 2.0±0.9 4.0±1.7
Flunarizni-treated 10 12.7±1.7 48.0±2.9 12.1±1.6 1.8±1.2 3.2±1.9

Table 6

Effect of LPTS on serum concentration of MDA and activity of SOD in aging mice induced by D-galactase ($\bar{x}±S$)"

Group n SOD (μU/L) MDA (μmol/L)
NS-treated 10 305.0±102.0 28.3±19.1
Untreated 10 154.9±103.9 47.2±21.8
Low-dose LPTS-treated 10 302.1±115.9 34.2±14.6
Medium-dose LPTS-treated 10 306.1±69.7* 29.7±9.0
High-dose LPTS-treated 10 313.9±128.6* 23.6±13.6*
Shuxuening-treated 10 335.2±137.5* 25.9±19.4*
Jiaogulanosidi-treated 10 248.3±172.9 34.8±20.4
VE-treated 10 250.8±135.8 39.3±15.3
Flunarizni-treated 10 235.9±168.8 39.2±11.2

Table 7

Effect of LPTS on concentration of GSH-Px in the liver of aging mice induced by D-galactase ($\bar{x}±S$, kU/L)"

Group n GSH-Px
NS-treated 10 49.2±2.6
Untreated 10 35.7±4.5△
Low-dose LPTS-treated 10 38.2±7.3
Medium-dose LPTS-treated 10 45.0±5.1
High-dose LPTS-treated 10 48.2±8.0*
Shuxuening-treated 10 38.0±7.2
Jiaogulanosidi-treated 10 47.8±11.1*
VE-treated 10 45.7±10.2
Flunarizni-treated 10 34.4±6.6

Table 8

Effect of LPTS on brain tissue homogenate concentration of lipofuscin and activity of MAO in aging mice induced by D-galactase ($\bar{x}±S$)"

Group n MAO[U/(mg·h)] Lipofuscin (μg/g)
NS-treated 10 6.1±3.9 0.5±0.4
Untreated 10 10.3±4.3 2.4±1.8
Low-dose LPTS-treated 10 11.0±5.8 2.0±2.0
Medium-dose LPTS-treated 10 9.6±5.4 0.5±0.7*
High-dose LPTS-treated 10 4.6±2.4* 0.3±0.3*
Shuxuening-treated 10 1.8±0.7* 0.4±0.4*
Jiaogulanosidi-treated 10 5.0±5.2* 1.2±1.8
VE-treated 10 2.0±0.8* 1.5±1.1
Flunarizni-treated 10 5.4±3.0* 1.9±2.4
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