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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 190-193.doi: 10.3736/jcim20080217

• Original Experimental Research • Previous Articles     Next Articles

Impact of the method of cooling blood to remove stasis on tumor necrosis factor-alpha and interleukin-10 mRNA expressions in peripheral blood mononuclear cells of rats with experimental autoimmune thyroiditis

Hong Zhang, Xiao-guang Xu, Jun Gu()   

  1. Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2007-05-11 Online:2008-02-20 Published:2008-02-15
  • Contact: GU Jun

Objective:To explore the effects of Liangxue Huayu therapy (LXHYT), a traditional Chinese herbal therapy for cooling blood and dissipating blood stasis, on rat model of experimental autoimmune thyroiditis (EAT) and its impact on tumor necrosis factor-alpha and interleukin-10 mRNA expressions in peripheral blood mononuclear cells.

Methods:Forty Wistar rats were randomly divided into five groups: normal control group, untreated EAT group, cyclosporine A (CyA)-treated group, Tripterygium glycosides-treated group and Liangxue Huayu Recipe (LXHYR)-treated group. The interventions were given by gavage to the rats in different groups once a day. All rats were sacrificed after 4-week treatment, and the level of serum thyroglobulin antibodies (TgAb) and the changes of histological grade of thyroid specimen were assessed by blind evaluation. Expressions of tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) mRNAs in peripheral blood mononuclear cells were assessed by real-time reverse transcription polymerase chain reaction method.

Results:There was a significant increase in the serum TgAb level and severe inflammatory infiltration in the untreated group. Expression of TNF-α mRNA in peripheral blood mononuclear cells was increased, while the expression of IL-10 mRNA was decreased in the untreated group (P<0.01). Compared with the untreated group, CyA, Tripterygium glycosides and LXHYF could decrease the serum TgAb level (P<0.05), but the three interventions showed no significant improvement in thyroid inflammation (P>0.05). TNF-α mRNA expression was decreased, while IL-10 mRNA expression was increased as compared with the untreated group (P<0.05).

Conclusion:LXHYT can decrease the serum TgAb level and recover the balance of Th1/Th2. This may provide an experimental basis for further research of assessing the antipsoriatic effect of Chinese herbal drugs with a rat model of EAT as an alternative model of psoriasis in vivo.

Key words: psoriasis, Chinese herbal drugs, experimental animal models, Thl cells, autoimmune thyroidits, rats

CLC Number: 

  • R581.4

Table 1

Severity of inflammatory infiltration in thyroid in different groups of rats"

Group n Number of histological grade of thyroid specimen
Normal (grade 0) Mild (grade 1) Moderate (grade 2) Severe (grade 3-4) Total (>2)
Normal control 8 7 1 0 0 0
EAT 8 0 1 2 5 7**
CyA-treated 8 1 2 2 3 5*
Tripterygium glycosides-treated 8 0 1 3 4 7**
Chinese herbal medicine-treated 8 0 1 4 3 7**

Table 2

Levels of TgAb and expressions of TNF-α and IL-10 mRNAs in peripheral blood mononuclear cells in different groups of rats ($\bar{x}$±s)"

Group n TgAb (ng/L) TNF-α/β-actin IL-10/β-actin
Normal control 8 0.420 7±0.137 3 10.216 4±2.448 4 11.035 8±3.450 7
EAT 8 1.274 4±0.079 2** 20.323 9±5.692 2** 4.161 3±1.331 8**
CyA-treated 8 0.862 5±0.115 7**△△ 5.826 9±2.570 8△△ 12.372 0±2.065 8△△
Tripterygium glycosides-treated 8 0.922 5±0.122 6**△△ 3.658 9±2.024 5* 7.659 8±1.752 2*△▲
Chinese herbal medicine-treated 8 0.906 2±0.101 8**△△ 12.755 8±2.088 8**△△▲□□ 7.733 0±1.874 5*△▲
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