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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 366-371.doi: 10.3736/jcim20080408

• Original Experimental Research • Previous Articles     Next Articles

Therapeutic effects of Tiaozhong Granule on esophagus mucous membrane in rats with mixed reflux esophagitis and the mechanisms

Zhao-hong Shi1(), Wen Su1, Jie-mei Zhang1, Jian-jun Hao1, Yun-xia Feng1, Zhou Chen2   

  1. 1. Department of Gastroenterology, Wuhan No. 1 Hospital, Wuhan, Hubei Province 430022, China
    2. School of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430065, China
  • Received:2007-09-29 Online:2008-04-20 Published:2008-04-15
  • Contact: SHI Zhao-hong E-mail:ycfyx2000@126.com

Objective: To explore the mechanism of Tiaozhong Granule (TZG), a compound traditional Chinese herbal medicine, in treating rats with mixed reflux esophagitis.
Methods: Fifty-eight SD rats were randomly divided into untreated group (n=12), sham-operated group (n=10), TZG-treated group (n=12), Banxia Xiexin Decoction (BXXXD)-treated group (n=12) and cisapride-treated group (n=12). Mixed reflux esophagitis was induced by esophago-duodenum end-to-side anastomosis. Four weeks later, the rats were orally administered twice daily for 12 days. Pathological changes of esophagus mucous membrane were observed by using HE staining. The expressions of proliferating cell nuclear antigen (PCNA) and p53 in the esophagus tissue were detected by immunohistochemical SABC method. Spectrophotometric method was used to detect the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum.
Results: Compared with the untreated group, pathological changes of esophagus mucous membrane were relieved in different degrees in TZG-treated group, BXXXD-treated group and cisapride-treated group. Content of MDA and expressions of PCNA and p53 were obviously decreased in the three treated groups (P<0.01), and the activities of SOD and GSH-Px were significantly increased in the three treated groups (P<0.05, P<0.01). TZG had better effects than cisapride in decreasing the content of MDA and increasing the activities of SOD and GSH-Px (P<0.05). TZG was better in aspect of reducing the expressions of PCNA and p53 than BXXXD and cisapride tablets (P<0.05).
Conclusion: Tiaozhong Granule can treat mixed reflux esophagitis in rats, and its action mechanisms may be associated with decreasing the expressions of PCNA and p53 in esophagus mucous membrane, reducing the content of MDA and increasing the activities of SOD and GSH-Px in serum.

Key words: esophagitis, reflux, compound recipe (TCD), oxygen radicals, proliferating cell nuclear antigen, tumor suppressor protein p53, rats

CLC Number: 

  • R571

Figure 1

Pathological changes of esophagus mucous membrane in five groups(HE staining, ×400) A: TZG-treated group; B: BXXXD-treated group; C: Cisapride-treated group; D: Untreated group; E: Sham-operated group."

Table 1

Pathological grading of esophagus mucous membrane in five groups"

Group n Cases in different degrees of pathological grading
Normal Mild Moderate Severe R
Sham-operated group 10 10 0 0 0 0.932 5
Untreated group 9 0 1 5 3 0.432 1**
Cisapride-treated group 8 5 1 2 0 0.552 1
BXXXD-treated group 9 6 2 1 0 0.786 5
TZG-treated group 10 8 2 0 0 0.853 2

Table 2

Serum content of MDA and activities of SOD and GSH-Px in five groups ($\bar{x}$±s )"

Group n SOD (U/L) MDA (μmol/L) GSH-Px (U/L)
Sham-operated group 10 466.15±13.70 3.62±0.18 141.64±3.88
Untreated group 9 345.02±24.11** 5.77±0.16** 112.77±6.20**
Cisapride-treated group 8 402.98±36.59△▲ 4.64±0.60△△▲ 118.26±6.34△▲▲
BXXXD-treated group 9 384.66±46.02△▲ 4.29±0.29△△ 122.78±4.76△△▲▲
TZG-treated group 10 431.94±21.87△△ 4.11±0.37△△ 135.83±3.71△△

Figure 2

PCNA positive cells in esophagus mucous membrane in five groups (Immunohistochemical SABC method, ×400) A: TZG-treated group; B: BXXXD-treated group; C: Cisapride-treated group; D: Untreated group; E: Sham-operated group."

Figure 3

p53 positive cells in esophagus mucous membrane in five groups (Immunohistochemical SABC method, ×400) A: TZG-treated group; B: BXXXD-treated group; C: Cisapride-treated group; D: Untreated group; E: Sham-operated group."

Table 3

Expressions of PCNA and p53 in esophagus mucous membrane in five groups ($\bar{x}$±s )"

Group n PCNA p53
Sham-operated group 10 72.09±8.01 63.34±7.48
Untreated group 9 106.64±12.25** 83.93±6.34**
Cisapride-treated group 8 88.11±8.46△△▲ 66.34±7.71△△▲
BXXXD -treated group 9 88.31±9.50△△▲ 71.81±8.34△△▲
TZG-treated group 10 84.80±4.29△△ 68.80±4.29△△
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