调中颗粒对大鼠混合反流性食管炎的治疗作用及其机制

doi:10.3736/jcim20080408

Abstract

Objective: To explore the mechanism of Tiaozhong Granule (TZG), a compound traditional Chinese herbal medicine, in treating rats with mixed reflux esophagitis.
Methods: Fifty-eight SD rats were randomly divided into untreated group (n=12), sham-operated group (n=10), TZG-treated group (n=12), Banxia Xiexin Decoction (BXXXD)-treated group (n=12) and cisapride-treated group (n=12). Mixed reflux esophagitis was induced by esophago-duodenum end-to-side anastomosis. Four weeks later, the rats were orally administered twice daily for 12 days. Pathological changes of esophagus mucous membrane were observed by using HE staining. The expressions of proliferating cell nuclear antigen (PCNA) and p53 in the esophagus tissue were detected by immunohistochemical SABC method. Spectrophotometric method was used to detect the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum.
Results: Compared with the untreated group, pathological changes of esophagus mucous membrane were relieved in different degrees in TZG-treated group, BXXXD-treated group and cisapride-treated group. Content of MDA and expressions of PCNA and p53 were obviously decreased in the three treated groups (P<0.01), and the activities of SOD and GSH-Px were significantly increased in the three treated groups (P<0.05, P<0.01). TZG had better effects than cisapride in decreasing the content of MDA and increasing the activities of SOD and GSH-Px (P<0.05). TZG was better in aspect of reducing the expressions of PCNA and p53 than BXXXD and cisapride tablets (P<0.05).
Conclusion: Tiaozhong Granule can treat mixed reflux esophagitis in rats, and its action mechanisms may be associated with decreasing the expressions of PCNA and p53 in esophagus mucous membrane, reducing the content of MDA and increasing the activities of SOD and GSH-Px in serum.

Key words: esophagitis, reflux, compound recipe (TCD), oxygen radicals, proliferating cell nuclear antigen, tumor suppressor protein p53, rats

CLC Number:

R571

Zhao-hong Shi, Wen Su, Jie-mei Zhang, Jian-jun Hao, Yun-xia Feng, Zhou Chen. Therapeutic effects of Tiaozhong Granule on esophagus mucous membrane in rats with mixed reflux esophagitis and the mechanisms. Journal of Chinese Integrative Medicine, 2008, 6(4): 366-371.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20080408

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References 12

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Group	n	Cases in different degrees of pathological grading
Group	n	Normal	Mild	Moderate	Severe	R
Sham-operated group	10	10	0	0	0	0.932 5
Untreated group	9	0	1	5	3	0.432 1**
Cisapride-treated group	8	5	1	2	0	0.552 1^△
BXXXD-treated group	9	6	2	1	0	0.786 5^△
TZG-treated group	10	8	2	0	0	0.853 2^△

Group	n	SOD (U/L)	MDA (μmol/L)	GSH-Px (U/L)
Sham-operated group	10	466.15±13.70	3.62±0.18	141.64±3.88
Untreated group	9	345.02±24.11**	5.77±0.16**	112.77±6.20**
Cisapride-treated group	8	402.98±36.59^△▲	4.64±0.60^△△▲	118.26±6.34^△▲▲
BXXXD-treated group	9	384.66±46.02^△▲	4.29±0.29^△△	122.78±4.76^△△▲▲
TZG-treated group	10	431.94±21.87^△△	4.11±0.37^△△	135.83±3.71^△△

Group	n	PCNA	p53
Sham-operated group	10	72.09±8.01	63.34±7.48
Untreated group	9	106.64±12.25**	83.93±6.34**
Cisapride-treated group	8	88.11±8.46^△△▲	66.34±7.71^△△▲
BXXXD -treated group	9	88.31±9.50^△△▲	71.81±8.34^△△▲
TZG-treated group	10	84.80±4.29^△△	68.80±4.29^△△

Therapeutic effects of Tiaozhong Granule on esophagus mucous membrane in rats with mixed reflux esophagitis and the mechanisms

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