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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 830-835.doi: 10.3736/jcim20080812

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Analgesic effects of electroacupuncture combined with Celebrex on rats with tibial cancer pain

Qi-liang Mao-ying1, Dian-huan Ren1, Wen-li Mi1, Qiong Liu1, Yan-qing Wang1,2,3()   

  1. 1. Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    2. Institute of Acupuncture Research, Institutes of Brain Science, Fudan University, Shanghai 200032, China
    3. State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
  • Received:2007-12-15 Online:2008-08-20 Published:2008-08-15

Objective: To establish a proper experimental model of bone cancer pain in rat for acupuncture research, and observe the pain-relieving effect of electroacupuncture (EA) and/or Celebrex on bone cancer pain in rats.
Methods: The rat model of bone cancer pain was established by percutaneous direct puncture technique and inoculating the rat mammary gland carcinoma cells Walker 256 into tibial medullary cavity directly, and evaluated by detecting the bone tumor growth and mechanical allodynia. The effects of daily EA treatment and/or Celebrex treatment on the rat mechanical allodynia after intratibial Walker 256 inoculation was observed in this study.
Results: Significant mechanical allodynia in ipsilateral hind paw and tumor growth in proximal end of tibial bone of rats in the untreated group were observed after intratibial Walker 256 inoculation. The mechanical allodynia thresholds in rats that received EA or 5 mg/(kg·d) Celebrex treatment showed no significant difference as compared with that of rats in the untreated group. However, the mechanical allodynia thresholds of rats in 10 mg/(kg·d) Celebrex group showed significant increase after 22- and 26-day treatment as compared with that in the methyl cellulose (MC) group. There was significant difference between rats with EA combined with 5 mg/(kg·d) Celebrex treatment and rats in the untreated group after 10-, 18- and 23-day treatment.
Conclusion: EA and 5 mg/(kg·d) Celebrex have synergistic effect on pain relieving and their combined use may enhance the analgesic effect on bone cancer pain.

Key words: bone cancer pain, electroacupuncture, Celebrex, analgesia, rats

CLC Number: 

  • R2-031

Figure 1

Tibial bone of a normal control rat and Walker 256 tumor-bearing rat 16 d after tumor inoculation"

Figure 2

Changes of mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain**P<0.01, vs normal group."

Figure 3

Effects of EA on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain"

Figure 4

Effects of different doses of Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs MC group."

Figure 5

Effects of EA combined with Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs untreated group."

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