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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 830-835.doi: 10.3736/jcim20080812

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Analgesic effects of electroacupuncture combined with Celebrex on rats with tibial cancer pain

Qi-liang Mao-ying1, Dian-huan Ren1, Wen-li Mi1, Qiong Liu1, Yan-qing Wang1,2,3()   

  1. 1. Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    2. Institute of Acupuncture Research, Institutes of Brain Science, Fudan University, Shanghai 200032, China
    3. State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
  • Received:2007-12-15 Online:2008-08-20 Published:2008-08-15

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Objective: To establish a proper experimental model of bone cancer pain in rat for acupuncture research, and observe the pain-relieving effect of electroacupuncture (EA) and/or Celebrex on bone cancer pain in rats.
Methods: The rat model of bone cancer pain was established by percutaneous direct puncture technique and inoculating the rat mammary gland carcinoma cells Walker 256 into tibial medullary cavity directly, and evaluated by detecting the bone tumor growth and mechanical allodynia. The effects of daily EA treatment and/or Celebrex treatment on the rat mechanical allodynia after intratibial Walker 256 inoculation was observed in this study.
Results: Significant mechanical allodynia in ipsilateral hind paw and tumor growth in proximal end of tibial bone of rats in the untreated group were observed after intratibial Walker 256 inoculation. The mechanical allodynia thresholds in rats that received EA or 5 mg/(kg·d) Celebrex treatment showed no significant difference as compared with that of rats in the untreated group. However, the mechanical allodynia thresholds of rats in 10 mg/(kg·d) Celebrex group showed significant increase after 22- and 26-day treatment as compared with that in the methyl cellulose (MC) group. There was significant difference between rats with EA combined with 5 mg/(kg·d) Celebrex treatment and rats in the untreated group after 10-, 18- and 23-day treatment.
Conclusion: EA and 5 mg/(kg·d) Celebrex have synergistic effect on pain relieving and their combined use may enhance the analgesic effect on bone cancer pain.

Key words: bone cancer pain, electroacupuncture, Celebrex, analgesia, rats

CLC Number: 

  • R2-031

Figure 1

Tibial bone of a normal control rat and Walker 256 tumor-bearing rat 16 d after tumor inoculation"

Figure 2

Changes of mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain**P<0.01, vs normal group."

Figure 3

Effects of EA on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain"

Figure 4

Effects of different doses of Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs MC group."

Figure 5

Effects of EA combined with Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs untreated group."

[1] Johnstone PA, Polston GR, Niemtzow RC , et al. Integration of acupuncture into the oncology clinic[J]. Palliat Med, 2002,16(3):235-239
doi: 10.1191/0269216302pm540oa pmid: 12047000
[2] Pan CX, Morrison RS, Ness J , et al. Complementary and alternative medicine in the management of pain, dyspnea, and nausea and vomiting near the end of life. A systematic review[J]. J Pain Symptom Manage, 2000,20(5):374-387
doi: 10.1016/S0885-3924(00)00190-1
[3] Fox A, Medhurst S, Courade JP , et al. Anti-hyperalgesic activity of the cox-2 inhibitor lumiracoxib in a model of bone cancer pain in the rat[J]. Pain, 2004,107(1-2):33-40
doi: 10.1016/j.pain.2003.09.003
[4] Zhu CB, Xu SF, Wu GC , et al. Research on combination of acupuncture with drugs[J]. World J Acup-Mox, 1997,7(4):54-59
[5] Medhurst SJ, Walker K, Bowes M , et al. A rat model of bone cancer pain[J]. Pain, 2002,96(1-2):129-140
doi: 10.1016/S0304-3959(01)00437-7 pmid: 11932069
[6] Mao-Ying QL, Zhao J, Dong ZQ , et al. A rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells[J]. Biochem Biophys Res Commun, 2006,345(4):1292-1298
doi: 10.1016/j.bbrc.2006.04.186
[7] Dixon WJ .Efficient analysis of experimental observations[J].Annu Rev Pharmacol Toxicol, 1980, 20: 441-462
doi: 10.1146/annurev.pa.20.040180.002301
[8] Mantyh PW, Clohisy DR, Koltzenburg M , et al. Molecular mechanisms of cancer pain[J]. Nat Rev Cancer, 2002,2(3):201-209
doi: 10.1038/nrc747 pmid: 11990856
[9] Mercadante S . Recent progress in the pharmacotherapy of cancer pain[J]. Expert Rev Anticancer Ther, 2001,1(3):487-494
doi: 10.1586/14737140.1.3.487 pmid: 12113115
[10] Mao-Ying QL, Wang YQ, Wu GC . Experimental research progress in cancer pain[J]. Guo Wai Yi Xue Sheng Li Bing Li Ke Xue Yu Lin Chuang Fen Ce, 2004,24(2):172-174
doi: 10.3969/j.issn.1673-2588.2004.02.021
毛应启梁, 王彦青, 吴根诚 . 癌症痛实验研究进展[J]. 国外医学生理病理科学与临床分册, 2004,24(2):172-174
doi: 10.3969/j.issn.1673-2588.2004.02.021
[11] Schwei MJ, Honore P, Rogers SD , et al. Neurochemical and cellular reorganization of the spinal cord in a murine model of bone cancer pain[J]. J Neurosci, 1999,19(24):10886-10897
doi: 10.1523/JNEUROSCI.19-24-10886.1999
[12] Honore P, Rogers SD, Schwei MJ , et al. Murine models of inflammatory, neuropathic and cancer pain each generates a unique set of neurochemical changes in the spinal cord and sensory neurons[J]. Neuroscience, 2000,98(3):585-598
doi: 10.1016/S0306-4522(00)00110-X
[13] Kehl LJ, Hamamoto DT, Wacnik PW , et al. A cannabinoid agonist differentially attenuates deep tissue hyperalgesia in animal models of cancer and inflammatory muscle pain[J]. Pain, 2003,103(1-2):175-186
doi: 10.1016/S0304-3959(02)00450-5
[14] Wacnik PW, Eikmeier LJ, Ruggles TR , et al. Functional interactions between tumor and peripheral nerve: morphology, algogen identification, and behavioral characterization of a new murine model of cancer pain[J]. J Neurosci, 2001,21(23):9355-9366
doi: 10.1523/JNEUROSCI.21-23-09355.2001
[15] Simpkins H, Lehman JM, Mazurkiewicz JE , et al. A morphological and phenotypic analysis of Walker 256 cells[J]. Cancer Res, 1991,51(4):1334-1338
doi: 10.1002/1097-0142(19910215)67:4+<1278::AID-CNCR2820671526>3.0.CO;2-U pmid: 1997171
[16] Shi B, Fu ZD, Lei XH , et al. Inhibitory effect of samarium-153-labeled ethylenediaminetetramethylene phosphonate(EDTMP) on bone invasion and osteolysis in Walker 256 carcinoma bearing rats[J]. Zhonghua Zhong Liu Za Zhi, 1997,19(6):423-426
施波, 傅招娣, 雷小虹 , 等. 153Sm-乙二胺四亚甲基膦酸对Walker256癌荷瘤大鼠骨侵袭骨溶解的抑制作用[J]. 中华肿瘤杂志, 1997,19(6):423-426
[17] Ao SQ, Yang XF, Zhao RC , et al. An animal model of cancer related hypercalcaemia induced by Walker256 carcinoma in rats[J]. Shanghai Shi Yan Dong Wu Ke Xue, 2000,20(1):37-39
敖淑清, 杨小芳, 赵人琤 , 等. Walker256癌肉瘤引发大鼠高血钙模型[J]. 上海实验动物科学, 2000,20(1):37-39
[18] Guo GL, Zhang XH, Yao ZX . The path of anti-mammary gland carcinoma by selective COX-2 inhibitor[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2000,20(1):37-39
郭贵龙, 张筱骅, 姚榛祥 . 选择性环氧化酶-2抑制剂的抗乳腺癌作用途径[J]. 中华实验外科杂志, 2000,20(1):37-39
[19] Mercadante S . The use of anti-inflammatory drugs in cancer pain[J]. Cancer Treat Rev, 2001,27(1):57-61
doi: 10.1053/ctrv.2000.0192 pmid: 11237777
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