SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 830-835.doi: 10.3736/jcim20080812

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Analgesic effects of electroacupuncture combined with Celebrex on rats with tibial cancer pain

Qi-liang Mao-ying1, Dian-huan Ren1, Wen-li Mi1, Qiong Liu1, Yan-qing Wang1,2,3()   

  1. 1. Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    2. Institute of Acupuncture Research, Institutes of Brain Science, Fudan University, Shanghai 200032, China
    3. State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
  • Received:2007-12-15 Online:2008-08-20 Published:2008-08-15

RichHTML

2

PDF (PC)

78

Like

Objective: To establish a proper experimental model of bone cancer pain in rat for acupuncture research, and observe the pain-relieving effect of electroacupuncture (EA) and/or Celebrex on bone cancer pain in rats.
Methods: The rat model of bone cancer pain was established by percutaneous direct puncture technique and inoculating the rat mammary gland carcinoma cells Walker 256 into tibial medullary cavity directly, and evaluated by detecting the bone tumor growth and mechanical allodynia. The effects of daily EA treatment and/or Celebrex treatment on the rat mechanical allodynia after intratibial Walker 256 inoculation was observed in this study.
Results: Significant mechanical allodynia in ipsilateral hind paw and tumor growth in proximal end of tibial bone of rats in the untreated group were observed after intratibial Walker 256 inoculation. The mechanical allodynia thresholds in rats that received EA or 5 mg/(kg·d) Celebrex treatment showed no significant difference as compared with that of rats in the untreated group. However, the mechanical allodynia thresholds of rats in 10 mg/(kg·d) Celebrex group showed significant increase after 22- and 26-day treatment as compared with that in the methyl cellulose (MC) group. There was significant difference between rats with EA combined with 5 mg/(kg·d) Celebrex treatment and rats in the untreated group after 10-, 18- and 23-day treatment.
Conclusion: EA and 5 mg/(kg·d) Celebrex have synergistic effect on pain relieving and their combined use may enhance the analgesic effect on bone cancer pain.

Key words: bone cancer pain, electroacupuncture, Celebrex, analgesia, rats

CLC Number: 

  • R2-031

Figure 1

Tibial bone of a normal control rat and Walker 256 tumor-bearing rat 16 d after tumor inoculation"

Figure 2

Changes of mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain**P<0.01, vs normal group."

Figure 3

Effects of EA on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain"

Figure 4

Effects of different doses of Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs MC group."

Figure 5

Effects of EA combined with Celebrex on mechanical allodynia of the ipsilateral hind paw in rats with tibial cancer pain *P<0.05, vs untreated group."

[1] Johnstone PA, Polston GR, Niemtzow RC , et al. Integration of acupuncture into the oncology clinic[J]. Palliat Med, 2002,16(3):235-239
doi: 10.1191/0269216302pm540oa pmid: 12047000
[2] Pan CX, Morrison RS, Ness J , et al. Complementary and alternative medicine in the management of pain, dyspnea, and nausea and vomiting near the end of life. A systematic review[J]. J Pain Symptom Manage, 2000,20(5):374-387
doi: 10.1016/S0885-3924(00)00190-1
[3] Fox A, Medhurst S, Courade JP , et al. Anti-hyperalgesic activity of the cox-2 inhibitor lumiracoxib in a model of bone cancer pain in the rat[J]. Pain, 2004,107(1-2):33-40
doi: 10.1016/j.pain.2003.09.003
[4] Zhu CB, Xu SF, Wu GC , et al. Research on combination of acupuncture with drugs[J]. World J Acup-Mox, 1997,7(4):54-59
[5] Medhurst SJ, Walker K, Bowes M , et al. A rat model of bone cancer pain[J]. Pain, 2002,96(1-2):129-140
doi: 10.1016/S0304-3959(01)00437-7 pmid: 11932069
[6] Mao-Ying QL, Zhao J, Dong ZQ , et al. A rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells[J]. Biochem Biophys Res Commun, 2006,345(4):1292-1298
doi: 10.1016/j.bbrc.2006.04.186
[7] Dixon WJ .Efficient analysis of experimental observations[J].Annu Rev Pharmacol Toxicol, 1980, 20: 441-462
doi: 10.1146/annurev.pa.20.040180.002301
[8] Mantyh PW, Clohisy DR, Koltzenburg M , et al. Molecular mechanisms of cancer pain[J]. Nat Rev Cancer, 2002,2(3):201-209
doi: 10.1038/nrc747 pmid: 11990856
[9] Mercadante S . Recent progress in the pharmacotherapy of cancer pain[J]. Expert Rev Anticancer Ther, 2001,1(3):487-494
doi: 10.1586/14737140.1.3.487 pmid: 12113115
[10] Mao-Ying QL, Wang YQ, Wu GC . Experimental research progress in cancer pain[J]. Guo Wai Yi Xue Sheng Li Bing Li Ke Xue Yu Lin Chuang Fen Ce, 2004,24(2):172-174
doi: 10.3969/j.issn.1673-2588.2004.02.021
毛应启梁, 王彦青, 吴根诚 . 癌症痛实验研究进展[J]. 国外医学生理病理科学与临床分册, 2004,24(2):172-174
doi: 10.3969/j.issn.1673-2588.2004.02.021
[11] Schwei MJ, Honore P, Rogers SD , et al. Neurochemical and cellular reorganization of the spinal cord in a murine model of bone cancer pain[J]. J Neurosci, 1999,19(24):10886-10897
doi: 10.1523/JNEUROSCI.19-24-10886.1999
[12] Honore P, Rogers SD, Schwei MJ , et al. Murine models of inflammatory, neuropathic and cancer pain each generates a unique set of neurochemical changes in the spinal cord and sensory neurons[J]. Neuroscience, 2000,98(3):585-598
doi: 10.1016/S0306-4522(00)00110-X
[13] Kehl LJ, Hamamoto DT, Wacnik PW , et al. A cannabinoid agonist differentially attenuates deep tissue hyperalgesia in animal models of cancer and inflammatory muscle pain[J]. Pain, 2003,103(1-2):175-186
doi: 10.1016/S0304-3959(02)00450-5
[14] Wacnik PW, Eikmeier LJ, Ruggles TR , et al. Functional interactions between tumor and peripheral nerve: morphology, algogen identification, and behavioral characterization of a new murine model of cancer pain[J]. J Neurosci, 2001,21(23):9355-9366
doi: 10.1523/JNEUROSCI.21-23-09355.2001
[15] Simpkins H, Lehman JM, Mazurkiewicz JE , et al. A morphological and phenotypic analysis of Walker 256 cells[J]. Cancer Res, 1991,51(4):1334-1338
doi: 10.1002/1097-0142(19910215)67:4+<1278::AID-CNCR2820671526>3.0.CO;2-U pmid: 1997171
[16] Shi B, Fu ZD, Lei XH , et al. Inhibitory effect of samarium-153-labeled ethylenediaminetetramethylene phosphonate(EDTMP) on bone invasion and osteolysis in Walker 256 carcinoma bearing rats[J]. Zhonghua Zhong Liu Za Zhi, 1997,19(6):423-426
施波, 傅招娣, 雷小虹 , 等. 153Sm-乙二胺四亚甲基膦酸对Walker256癌荷瘤大鼠骨侵袭骨溶解的抑制作用[J]. 中华肿瘤杂志, 1997,19(6):423-426
[17] Ao SQ, Yang XF, Zhao RC , et al. An animal model of cancer related hypercalcaemia induced by Walker256 carcinoma in rats[J]. Shanghai Shi Yan Dong Wu Ke Xue, 2000,20(1):37-39
敖淑清, 杨小芳, 赵人琤 , 等. Walker256癌肉瘤引发大鼠高血钙模型[J]. 上海实验动物科学, 2000,20(1):37-39
[18] Guo GL, Zhang XH, Yao ZX . The path of anti-mammary gland carcinoma by selective COX-2 inhibitor[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2000,20(1):37-39
郭贵龙, 张筱骅, 姚榛祥 . 选择性环氧化酶-2抑制剂的抗乳腺癌作用途径[J]. 中华实验外科杂志, 2000,20(1):37-39
[19] Mercadante S . The use of anti-inflammatory drugs in cancer pain[J]. Cancer Treat Rev, 2001,27(1):57-61
doi: 10.1053/ctrv.2000.0192 pmid: 11237777
[1] Yen-Lin Chao, Yi-Ai Rau, Hong-Sheng Shiue, Jiun-Lin Yan, Yuan-Yun Tang, Shao-Wen Yu, Bo-Yan Yeh, Yen-Lung Chen, Tsung-Hsien Yang, Shu-Chen Cheng, Yi-Wen Hsieh, Hsin-Chia Huang, Fu-Kuang Tsai, Yu-Sheng Chen, Geng-Hao Liu. Using a consensus acupoints regimen to explore the relationship between acupuncture sensation and lumbar spinal postoperative analgesia: A retrospective analysis of prospective clinical cooperation. Journal of Integrative Medicine, 2022, 20(4): 329-337.
[2] Xiang-yu Wei, Shi-lei Luo, HuiChen, Shan-shan Liu, Zhi-gang Gong, Song-huaZhan. Functional connectivity changes during migraine treatment with electroacupuncture at Shuaigu (GB8). Journal of Integrative Medicine, 2022, 20(3): 237-243.
[3] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[4] Luciana Aikawa, Alexandre Massao Yoshizumi, Mirian Matsura Shirassu, Marcia Kiyomi Koike. Rapid acupuncture for musculoskeletal pain in the emergency room of the Hospital Servidor Publico Estadual, Brazil: A quasi-experimental study. Journal of Integrative Medicine, 2020, 18(4): 313-318.
[5] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[6] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[7] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[8] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[9] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[10] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[11] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[12] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[13] Meng-yue Liu, Cheng-wei Wang, Zhou-peng Wu, Ning Li. Electroacupuncture for the prevention of postoperative gastrointestinal dysfunction in patients undergoing vascular surgery under general anesthesia: Study protocol for a prospective practical randomized controlled trial. Journal of Integrative Medicine, 2014, 12(6): 512-519.
[14] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[15] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3