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Journal of Chinese Integrative Medicine ›› 2013, Vol. 11 ›› Issue (5): 337-342.doi: 10.3736/jintegrmed2013042

• Research Article • Previous Articles     Next Articles

Effects of rutin on oxidative stress in mice with kainic acid-induced seizure

Marjan Nassiri-Asla(), Taghi Naserpour Farivarb, Esmail Abbasic, Hamid Reza Sadeghniad, Mehdi SheikhieMina Lotfizadehf, Parisa Bazahangf   

  1. a. Cellular and Molecular Research Centre, Department of Pharmacology, Qazvin University of Medical Sciences, Qazvin, Iran
    b. Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran
    c. Department of Pharmacology, Qazvin University of Medical Sciences, Qazvin, Iran
    d. Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
    e. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    f. School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
  • Received:2013-03-04 Accepted:2013-05-27 Online:2013-09-10 Published:2013-09-15


Flavonoids are present in foods such as fruits and vegetables. Several studies have demonstrated a relationship between the consumption of flavonoid-rich foods and prevention of human disease, including neurodegenerative disorders. We assessed the effect of rutin (quercetin-3-O-rutinoside) on oxidative stress in kainic acid (KA)-induced seizure.


Thirty-six BALB/c mice were randomly divided into three groups. In the control group, saline (intra-peritoneal, i.p.) was administered for 7 d, and on the last day, KA (10 mg/kg, i.p.) was injected 30 min after administration of saline. In rutin groups, mice were pretreated with rutin (100 and 200 mg/kg, i.p.) for 7 d, and on the last day, KA (10 mg/kg, i.p.) was injected 30 min after administration of rutin. Subsequently, behavioural changes were observed in mice. Lipid peroxidation and oxidative stress were measured respectively in the early and late phases after KA-induced seizures.


Seizure scores in the rutin groups were significantly lower than those in the control group (P < 0.01). Furthermore, rutin dose-dependently inhibited the number of wet-dog shakes (WDS) (P<0.05). Malondialdehyde level in the hippocampus of the rutin groups was significantly lower than that in the hippocampus of the control group on days 1 and 21 after KA administration. In the rutin groups, the thiol levels observed on day 1 after KA administration were highe+r than that in the control group (P < 0.01).


These results indicate that rutin has potential anticonvulsant and antioxidative activities against oxidative stress in KA-induced seizure in mice.

Key words: Plant extracts, Rutin, Kainic acid, Oxidative stress, Epilepsy, Seizure, Mice

Figure 1

The effects of rutin (100 and 200 mg/kg) on seizure score (A), onset of WDS (B) and the number of WDS (C) in mice with KA-induced seizure The control group was administered saline (i.p.) for 7 d, and on the last day, KA (10 mg/kg, i.p.) was injected 30 min after administration of saline. Rutin (100 and 200 mg/kg, i.p.) was administered for 7 d, and on the last day KA (10 mg/ kg, i.p.) was injected 30 min after the administration of rutin. Data are expressed as mean ± standard error of mean. *P < 0.05, **P < 0.01, vs control group, n=36, analyzed by post-hoc Tukey’s test. KA: kainic acid; WDS: wet-dog shake."

Figure 2

Effect of rutin on MDA in the hippocampus on days 1 and 21 after kainic acid administration Values are expressed as mean ± standard error of mean. *P < 0.05, **P < 0.01, vs control group, n=6, analyzed by post-hoc Tukey’s test. MDA: malondialdehyde."

Figure 3

Effect of rutin on total thiol concentrations in the hippocampus on days 1 and 21 after kainic acid administrationValues are expressed as mean ± standard error of mean. **P < 0.01, compared to control group, n=6, analyzed by post-hoc Tukey’s test."

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