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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 541-545.doi: 10.3736/jcim20090609

• Original Experimental Research • Previous Articles     Next Articles

Anti-inflammatory effects and quantitative study of the combinations of active ingredients of Painong powder in mice

 Jun-chao Chen, Lu-jin Li, Shi-mei Wen, Ying-chun He, Hong-xia Liu, Qing-shan Zheng   

  1. Center for Drug of Clinical Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2008-12-22 Accepted:2009-03-31 Online:2009-06-20 Published:2009-06-15


To study the anti-inflammatory effects of the combinations of active components of Painong powder, a compound traditional Chinese herbal medicine, and the quantitative analysis of their interactions.

The mouse model of acute inflammation with increase of capillary permeability was induced by intraperitoneal injection of acetic acid. An orthogonal design with 2 levels (used and unused) was applied to assign the combinations groups of active ingredients including naringin and neohesperidin, peoniflorin, and platycodin. Aspirin and normal saline were administered as control. The pharmacodynamic interactions were analyzed by the optical density (OD) of infiltrated Evans blue.

The different combinations of active ingredients showed anti-inflammatory effect with different degree, and the predicted values of OD varied from 0.115 to 0.170. The maximum anti-inflammatory effect was from the combination of naringin, neohesperidin, paeoniflorin and platycodin, better than that of the saline group (P<0.01). However, there was no significant difference as compared with the aspirin group (P>0.05). Paeoniflorin showed a dominant contribution to the formula, and platycodin the least. The combination of all active components exhibited synergism.

The results suggest that all the ingredients are efficacious constituents of the formula, and paeoniflorin shows a dominant contribution to the formula. More information about prescription compatibility can be obtained by the orthogonal simulation method.

Key words: Painong powder, Active ingredient, Compatibility (traditional Chinese drug), Drug interactions

Table 1

Two levels of active components in the orthogonal design(mg/kg)"

Factor A: Paeoniflorin B: Naringin+neohesperidin C: Platycodin
1 70 490+35 90
2 0 0 0

Table 2

Effects of different combinations on capillary permeability(x±s)"

Group ABC n OD value
1 +++ 10 0.115±0.037**
2 ++– 10 0.119±0.032**
3 +–– 10 0.129±0.044**
4 +–+ 10 0.158±0.056**
5 –++ 10 0.153±0.036**
6 –+– 10 0.160±0.051**
7 ––+ 10 0.170±0.042**
8 ––– 10 0.240±0.060
9 Aspirin 10 0.106±0.026**

Table 3

Pharmacodynamic parameters of combination"

Parameter Value RSE Note
Ebase 0.240 0.079 Baseline of response
ED (A) –0.051 0.214 Response of paeoniflorin
ED (B) –0.038 0.289 Response of naringin and neohesperidin
ED (C) –0.013 0.841 Response of platycodin
Emax 0.115 0.079 Maximum predicted response
Emin 0.170 0.064 Minimum predicted response
Ecut –0.024 Critical value of effective
σ 0.043 Errors from calculation
and experiment

Table 4

Relationship of NEB and the results of the two one-side t test"

ED –0.051 –0.038 –0.013
90% CI (%) 62-82 68-89 81-103
NEB (%) 90-110 90-110 90-110
Conclusion P P P

Table 5

Characteristics of drug interactions and their responses (Eint)"

Combination scheme Eint RSE Q value Interaction
ABC 0.136 0.086 6.4 Synergism
AB 0.069 0.147 –1.4 Antagonism
AC 0.099 0.179 –1.7 Antagonism
BC 0.063 0.179 –0.7 Antagonism

Table 6

Prediction of OD value in different combinations of the active ingredients"

Combination scheme n Predicted OD value Observed OD value PE (%) Note
ABC 10 0.115 Emax
C 10 0.170 Emin
ABC 10 0.115 0.115 0 Group 1
AB 10 0.126 0.119 6.6 Group 2
A 10 0.129 0.129 0 Group 3
AC 10 0.136 0.158 14.1 Group 4
BC 10 0.167 0.153 9.5 Group 5
B 10 0.160 0.160 0 Group 6
C 10 0.170 0.170 0 Group 7

Figure 1

Scatter plot of the fit goodness of simulation (PE1) and the dispersion of observed responses (PE2) It is perfect when all points located into Evaluation Window (dashed). (A) 76% points of 7 experimental groups (n=10 for each group) were located into the Evaluation Window. The result suggested the reliability of the model. (B) Enlarged the sample size to 700 (n=100 for each group) by Monte Carlo simulation, 567 of 700 points (81%) were located into the Evaluation Window. It revealed the stability of the model."

[1] Kano Y, Saito KI, Masao KG . Pharmacological properties of galenical preparations(Ⅳ). Effect of Chinese medicinal prescriptions "Haino-to" and "Haino-san" on mouse carbuncle induced by Staphylococcus aureus 226[J]. Nat Med, 1979,33(4):189-197
[2] Chen JC, Li LJ, Wen SM, Zheng QS . Anti-inflammatory effect and quantitative analysis of combinations of Painong powder in mice[J]. Zhongguo Shi Yan Fang Ji Xue Za Zhi, 2008,14(9):33-37
陈君超, 李禄金, 文世梅, 郑青山 . 排脓散在小鼠的抗炎作用及其方剂配伍的定量分析[J]. 中国实验方剂学杂志, 2008,14(9):33-37
[3] Wang ZJ, Chen MZ, Sun GH, Xu SY . Effect of total glucoside of paeony on rheumatoid arthritis patients[J]. Zhongguo Yao Li Xue Tong Bao, 1994,10(2):117-122
王志坚, 陈敏珠, 孙桂华, 徐叔云 . 白芍总甙治疗类风湿性关节炎的临床药理研究[J]. 中国药理学通报, 1994,10(2):117-122
[4] Liang JS, Chen MZ, Xu SY . Effect of total glycosides of paeony on peritoneal macrophage functions in rats[J]. Zhongguo Yao Li Xue Yu Du Li Xue Za Zhi, 1990,4(2):153-154
梁君山, 陈敏珠, 徐叔云 . 白芍总甙对大鼠腹腔巨噬细胞功能的影响[J]. 中国药理学与毒理学杂志, 1990,4(2):153-154
[5] Lee JH, Choi YH, Kang HS, Choi BT . An aqueous extract of Platycodi radix inhibits LPS-induced NF-kappaB nuclear translocation in human cultured airway epithelial cells[J]. Int J Mol Med, 2004,13(6):843-847
[6] Kim YP, Lee EB, Kim SY, Li D, Ban HS, Lim SS, Shin KH, Ohuchi K . Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum[J]. Planta Med, 2001,67(4):362-364
doi: 10.1055/s-2001-14317
[7] Kano Y, Saito KI, Miura G, Masao KG . Pharmacological properties of galenical preparations(Ⅹ). Inhibitory components on carrageenin edema formation in Chinese medicine prescription "Haino-san"[J]. Nat Med, 1983,37(3):204-208
[8] Li XF, Xue QP, Dong CM . Determination of total saponins in Radix Platycodon grandiflorum by colorimetry[J]. Zhong Yi Yao Xue Kan, 2006,24(12):2232-2234
李喜凤, 薛秋萍, 董诚明 . 桔梗中总皂苷的含量测定[J]. 中医药学刊, 2006,24(12):2232-2234
[9] Liu F, Yang GD . Extraction of paeoniflorin in Radix Paeoniae Alba[J]. Zhong Cheng Yao, 2003,25(10):792-795
刘芳, 杨广德 . 白芍中芍药苷的提取方法研究[J]. 中成药, 2003,25(10):792-795
[10] Jia Q, Bai Y, Ma Y, Peng W, Su WW . Chemical constituents of Fructus Aurantii and Fructus Aurantii Immaturus by HPLC-ESI-MS[J]. Zhong Cao Yao, 2005,36(2):169-172
贾强, 白杨, 马燕, 彭维, 苏薇薇 . 枳壳和枳实化学成分的HPLC-ESI-MS分析[J]. 中草药, 2005,36(2):169-172
[11] Chen Q. Technology of research on pharmacology of traditional Chinese medicine(2nd ed)[M]. Beijing: People's Medical Publishing House, 2006,( 2nd ed):300-301
陈奇 . 中药药理研究方法学(第2版)[M]. 北京: 人民卫生出版社, 2006,( 第2版):300-301
[12] Zheng QS, He YC, Yang J, Xu L, Lu YH, Liu HX, Chen JC, Sun RY . An approach for the analysis of pharmacodynamic interactions and the simulation of combined response[J]. Zhongguo Yao Li Xue Tong Bao, 2007,23(8):1106-1112
郑青山, 何迎春, 杨娟, 许羚, 吕映华, 刘红霞, 陈君超, 孙瑞元 . 药物相互作用分析与复方药效模拟: 一个方法学研究[J]. 中国药理学通报, 2007,23(8):1106-1112
[13] Wang BX. Modern pharmacology and clinic of Chinese traditional medicine[M]. Tianjin: Tianjin Science & Technology Translation & Publishing Co.,2004: 490-493
王本祥 . 现代中药药理与临床[M]. 天津: 天津科技翻译出版公司,2004: 490-493
[14] Chen JC, Pan YJ, Li XS, Sun J, Liu HX, Sun RY, Zheng QS . Quantitative approach for calculation of pharmacodynamic interactions and simulation of combined response[J]. Zhongguo Zhong Yao Za Zhi, 2008,33(16):2029-2032
陈君超, 潘友俊, 李兴珊, 孙杰, 刘红霞, 孙瑞元, 郑青山 . 药效学相互作用的定量计算方法与模拟研究[J]. 中国中药杂志, 2008,33(16):2029-2032
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