祛湿化瘀方对脂肪肝大鼠脂联素-游离脂肪酸代谢路径的影响

doi:10.3736/jcim20090610

Abstract

Objective

To explore the effects of Qushi Huayu Decoction (QSHYD), a compound traditional Chinese herbal medicine, in prevention and treatment of non-alcoholic fatty liver disease (NAFLD) in rats.
Methods

Forty Wistar male rats were used to establish the NAFLD model by subcutaneous injection of carbon tetrachloride (CCl₄) for 4 weeks (twice weekly) along with high-fat and low-protein diet for 2 weeks. After two-week administration, the rats were randomly divided into four groups: untreated group, high-dose QSHYD group, medium-dose QSHYD group and low-dose QSHYD group. Another six rats were used as normal control. After 2-week treatment, the following indexes were detected: (1) liver pathology; (2) contents of serum adiponectin (ADP) and liver triglyceride (TG); (3) concentrations of liver FFA, adiponectin receptor 2 (AdipoR2), malonyl-coenzyme A (malony1-CoA), AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACCase), fatty acid synthase (FAS) and carnitine palmitoyl transferase-1 (CPT-1).
Results

Compared with the normal group, there were physiological changes associated with hepatic steatosis and inflammation in liver tissues in the untreated group as observed by oil red O staining and HE staining. The TG, FFA, malony1-CoA, FAS, and ACCase concentrations in liver tissues in the untreated group were elevated significantly. While the contents of ADP in serum and AdipoR2, CPT-1 and AMPK in liver tissues in the untreated group were decreased markedly. The pathological damages in each QSHYD-treated group were significantly less than those in the untreated group. The TG and FFA contents in liver tissues in each QSHYD-treated group were significantly decreased. The FAS, ACCase and malonyl-CoA concentrations in liver tissues of the high QSHYD-treated group were reduced markedly as compared with the untreated group. High- and medium-dose of QSHYD could significantly increase ADP content in serum and AMPK, CPT-1 and AdipoR2 contents in liver tissues.
Conclusion

QSHYD can affect the ADP-FFA pathway by increasing the content of serum ADP, which may be one of its important mechanisms in preventing and treating NAFLD in rats.

Key words: Fatty liver, Free fatty acid, Adiponectin, AMP-activated protein kinase, Qushi Huayu Decoction, Rats

Hong-shan Li, Qin Feng, Li-li Xu, Shao-dong Chen, Xue-mei Li, Yi-yang Hu. Effects of Qushi Huayu Decoction in prevention and treatment of fatty liver in rats based on adiponection-free fatty acid pathway. Journal of Chinese Integrative Medicine, 2009, 7(6): 546-551.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090610

http://www.jcimjournal.com/EN/Y2009/V7/I6/546

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References 12

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Group	n	TG (mg/g)	FFA (μmol/g)
Normal	6	8.7±2.6**	56.7±5.6**
Untreated	10	33.2±9.7	202.7±54.2
Low-dose QSHYD	8	22.7±5.8**	117.5±17.9**
Medium-dose QSHYD	9	19.9±3.7**	115.8±24.6**
High-dose QSHYD	10	19.6±4.0**	100.8±22.4**

Group	n	Number of rats with different degrees of hepatic steatosis
Group	n	F₀	F₁	F₂	F₃	F₄
Normal	6	6	0	0	0	0
Untreated	10	0	0	2	7	1
Low-dose group QSHYD	8	0	2	4	2	0
Medium-dose QSHYD	9	0	2	6	1	0
High-dose group QSHYD	10	0	3	5	2	0
Ridit score		0.069 8	0.220 9	0.441 9	0.779 1	0.988 4

Group	n	ADP (μg/mL)	AdipoR2 (ng/g)
Normal	6	25.7±3.8**	4 474±246**
Untreated	10	14.3±3.8	3 205±665
Low-dose QSHYD	8	17.7±5.1^△△	3 960±380**
Medium-dose QSHYD	9	20.1±5.4*	3 940±341**
High-dose QSHYD	10	23.9±4.5**	4 052±353**

Group	n	AMPK (ng/g)	ACCase (μg/g)	Malony1-CoA (μg/g)
Normal	6	418±26**	19±8**	270±30**
Untreated	10	219±78	79±31	511±88
Low-dose QSHYD	8	311±72*^△	64±29^△	421±97**^△
Medium-dose QSHYD	9	379±95**	60±18	409±43**
High-dose QSHYD	10	394±83**	39±19**	347±52**

Group	n	FAS	CPT-1
Normal	6	83±9**	2 119±199**
Untreated	10	152±29	1 480±275
Low-dose QSHYD	8	130±15^△	1 642±187
Medium-dose QSHYD	9	125±22*	1 679±173*
High-dose QSHYD	10	107±10**	1 716±171*

Effects of Qushi Huayu Decoction in prevention and treatment of fatty liver in rats based on adiponection-free fatty acid pathway

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