Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (8): 741-745.doi: 10.3736/jcim20090807

• Original Experimental Research • Previous Articles     Next Articles

Effects of Feiyanning Decoction on proliferation of lung adenocarcinoma cell line and their production of interleukin-6 and interleukin-8 induced by tumor necrosis factor-α

Ju-yong Wang,Zhan Zheng, Zhen-ye Xu, Jing Guo, Wan So   

  1. Cancer Institute of Traditional Chinese Medicine,Longhua Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200032,China
  • Received:2009-05-04 Accepted:2009-07-08 Online:2009-08-20 Published:2009-08-15
  • Contact: Ju-yong Wang E-mail:wangjuyong1@126.com

Objective

To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on proliferation of lung adenocarcinoma cell line A549 cells and their production of interleukin-6 (IL-6) and IL-8 induced by tumor necrosis factor-α (TNF-α).

Methods

A549 cells were incubated with rat serum containing Feiyanning Decoction at 15% for 24, 48 and 72 h respectively. The cell proliferation was examined by 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt assay (WST-8). The production of IL-6 and IL-8 was tested by enzyme-linked immunosorbent assay after 48-hour treatment of reagents, and the expressions of IL-6 and IL-8 mRNAs were detected by reverse transcription-polymerase chain reaction. 

Results

Serum containing Feiyanning Decoction had obvious inhibitive functions in A549 cell proliferation after 48- and 72-treatment. TNF-α (1 μg/L) strongly induced the production of IL-6 and IL-8 as compared with the control serum in A549 cells, and the induced cytokine production was significantly suppressed by 15% serum containing Feiyanning Decoction (P<0.01). In addition, serum containing Feiyanning Decoction could inhibit the mRNA expressions of IL-6 and IL-8 (P<0.01).
Conclusion

Feiyanning Decoction can inhibit IL-6 and IL-8 production induced by TNF-α. It is therefore expected to be a new strategy for treating lung cancer.

Key words: Feiyanning Decoction, Cytokine, Lung neoplasms, Serologic pharmacology, In vitro

"

Gene Primer sequence Size of PCR product (bp)
IL-6 Forward: 5'-GATGCTACCAAACTGGATATAATC-3' 269
Reverse: 5'-GGTCCTTAGCCACTCCTTCTGTG-3'
IL-8 Forward: 5'-CTGTGTGAAGCTGCAGTTCT-3' 180
Reverse: 5'-TAGGCAGACCTCGTTTCCAT-3'
β-actin Forward: 5'-AAGTACTCCGTGTGGATCGG-3' 547
Reverse: 5'-TCAAGTTGGGGGACAAAAAG-3'

"

Group n OD value Rate of inhibition after treatment (%)
24 h 48 h 72 h 24 h 48 h 72 h
Control serum 4 0.325±0.014 0.497±0.021 0.521±0.019 0 0 0
Serum containing Feiyanning
Decoction
4 0.344±0.028 0.359±0.020 0.321±0.022 0.05±0.62 30.85±1.62* 37.66±1.48**

Figure 1

Effects of serum containing Feiyanning Decoction on IL-6 and IL-8 levels induced by TNF-α in A549 cells Confluent cells were incubated for 48 h with or without 15% serum containing Feiyanning Decoction, or TNF-α (1 μg/L). Concentrations of IL-6 and IL-8 in supernatant were measured by ELISA. Bars represent $\bar{x}$±s (n=3). **P<0.01, vs TNF-α."

Figure 2

Effects of serum containing Feiyanning Decoction on IL-6 and IL-8 mRNA expressions in A549 cells After the treatment, RNA was extracted. Levels of cytokines and β-actin mRNAs were examined by RT-PCR. The three panels on left show the expression levels of IL-6, IL-8 and β-actin mRNAs; bar graphs at the right show the expression levels of IL-6 or IL-8 relative to those of β-actin. The experiments were performed in triplicate. The data were represented as $\bar{x}$±s (n=3). **P<0.01, vs TNF-α."

[1] Balkwill F, Mantovani A . Inflammation and cancer: back to Virchow?[J]. Lancet, 2001,357(9255):539-545
doi: 10.1016/S0140-6736(00)04046-0
[2] Mantovani A, Allavena P, Sica A, Balkwill F . Cancer-related inflammation[J]. Nature, 2008,454(7203):436-444
doi: 10.1038/nature07205
[3] Coussens LM, Werb Z . Inflammation and cancer[J]. Nature, 2002,420(6917):860-867
doi: 10.1038/nature01322
[4] Román J, Giménez A, Lluis JM, Gassó M, Rubio M, Caballeria J, Parés A, Rodés J, Fernández-Checa JC . Enhanced DNA binding and activation of transcription factors NF-kappa B and AP-1 by acetaldehyde in HEPG2 cells[J]. J Biol Chem, 2000,275(19):14684-14690
doi: 10.1074/jbc.275.19.14684
[5] Xu ZY, Jin CJ, Shen DY, Li M, Zhou WD, Zhu YW, Zhang M, Deng HB, Wang ZQ, Li L, Shou WZ, Zheng Z . Clinical study on treatment of advanced non-small-cell lung cancer with Chinese herbal medicine in different stages combined with chemotherapy[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(10):874-878
doi: 10.3321/j.issn:1003-5370.2007.10.005
徐振晔, 金长娟, 沈德义, 李敏, 周卫东, 朱晏伟, 张铭, 邓海滨, 王中奇, 李蕾, 寿伟臻, 郑展 . 中医药分阶段结合化疗治疗晚期非小细胞肺癌的临床研究[J]. 中国中西医结合杂志, 2007,27(10):874-878
doi: 10.3321/j.issn:1003-5370.2007.10.005
[6] Wang JY, Xu ZY . Professor Xu Zhenye's experience in the treatment of lung cancer[J]. Liaoning Zhong Yi Za Zhi, 2007,34(9):1202-1203
王菊勇, 徐振晔 . 徐振晔教授治疗肺癌的经验[J]. 辽宁中医杂志, 2007,34(9):1202-1203
[7] Wang JY, Jin Y, Xu ZY, Zheng Z . Effects of Feiyanning Decoction on gene expression of nuclear factor-κB activated by tumor necrosis factor-α in lung adenocarcinoma cell line[J]. J Chin Integr Med, 2009,7(3):249-254
王菊勇, 金叶, 徐振晔, 郑展 . 肺岩宁方对肿瘤坏死因子α诱导的人肺癌细胞系核转录因子κB基因表达的影响[J]. 中西医结合学报, 2009,7(3):249-254
[8] Chen Q. Technology of research on pharmcology of traditional Chinese medicine[M]. Beijing: People's Medical Publishing House, 2000: 145
陈奇 . 中药药理研究方法学[M]. 北京: 人民卫生出版社, 2000: 145
[9] Li YK . Several issues of traditional Chinese medicine serological pharmacological experimental methods[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 1999,10(2):95-98
doi: 10.3321/j.issn:1003-9783.1999.02.011
李仪奎 . 中药血清药理学实验方法的若干问题[J]. 中药新药与临床药理, 1999,10(2):95-98
doi: 10.3321/j.issn:1003-9783.1999.02.011
[10] Cho WC . Latest discoveries and trends in translational cancer research: highlights of the 2008 Annual Meeting of the American Association for Cancer Research[J]. Technol Cancer Res Treat, 2008,7(4):269-277
doi: 10.1177/153303460800700401
[11] Dranoff G . Cytokines in cancer pathogenesis and cancer therapy[J]. Nat Rev Cancer, 2004,4(1):11-22
doi: 10.1038/nrc1252
[12] Blackwell TS, Christman JW . The role of nuclear factor-κB in cytokine gene regulation[J]. Am J Respir Cell Mol Biol, 1997,17(1):3-9
doi: 10.1165/ajrcmb.17.1.f132
[13] Cai Z, Chen Q, Chen J, Lu Y, Xiao G, Wu Z, Zhou Q, Zhang J . Monocyte chemotactic protein 1 promotes lung cancer-induced bone resorptive lesions in vivo[J]. Neoplasia, 2009,11(3):228-236
doi: 10.1593/neo.81282
[14] Esfandi F, Mohammadzadeh Ghobadloo S, Basati G . Interleukin-6 level in patients with colorectal cancer[J]. Cancer Lett, 2006,244(1):76-78
doi: 10.1016/j.canlet.2005.12.003
[15] Salgado R, Junius S, Benoy I, Van Dam P, Vermeulen P, Van Mark E, Huget P, Dirix LY . Circulating interleukin-6 predicts survival in patients with metastatic breast cancer[J]. Int J Cancer, 2003,103(5):642-646
doi: 10.1002/ijc.10833 pmid: 12494472
[1] Pádraic J.Dunne, Julie Lynch, Lucia Prihodova, Caoimhe O'Leary, Atiyeh Ghoreyshi, Sharee A. Basdeo, Donal J.Cox, Rachel Breen, Ali Sheikhi, Áine Carroll, Cathal Walsh, Geraldine McMahon, Barry White. Burnout in the emergency department: Randomized controlled trial of an attention-based training program. Journal of Integrative Medicine, 2019, 17(3): 173-180.
[2] J.M. Oliver-Baxter, H.S. Whitford, D.A. Turnbull, M.J. Bond. Effects of vitamin supplementation on inflammatory markers and psychological wellbeing among distressed women: A randomized controlled trial. Journal of Integrative Medicine, 2018, 16(5): 322-328.
[3] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
[4] Lilian Uchoa Carneiro, Ilna Gomes da Silva, Mirian Eliza Alves de Souza, Wellington da Silva Côrtes, Mário Geraldo de Carvalho, Bruno Guimarães Marinho. Antinociceptive and anti-inflammatory activities of leaf extracts from Annona tomentosa R.E.Fr. Journal of Integrative Medicine, 2017, 15(5): 379-387.
[5] Gloria Gronowicz, Eric R. Secor Jr., John R. Flynn, Liisa T. Kuhn. Human biofield therapy does not affect tumor size but modulates immune responses in a mouse model for breast cancer. Journal of Integrative Medicine, 2016, 14(5): 389-399.
[6] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[7] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[8] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
[9] Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[10] Li Cai-hong, Qiu Qing-hua, Wu Xing-wei, Gong Yuan-yuan, Xie Zheng-gao, Song Yi, Gu Qing. Chinese herbal medicine Lingqi Huangban Granule protects retinal pigment epithelial cells against oxidative stress-induced injury in vitro. Journal of Chinese Integrative Medicine, 2012, 10(1): 85-90.
[11] Jong-Min Kim, Kyung-Soon Kim, Yeon-Weol Lee, Chong-Kwan Cho, Hwa-Seung Yoo, Ji-Young Bang, Eung-Yoon Kim, In-Cheol Kang. Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis. Journal of Chinese Integrative Medicine, 2011, 9(9): 1005-1013.
[12] Zhao-hui Chu, Xiao-hua Liang, Xin-li Zhou, Ruo-fan Huang, Qiong Zhan, Jing-wei Jiang. Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway. Journal of Chinese Integrative Medicine, 2011, 9(5): 533-538.
[13] Saili Paul, Soumya Sundar Bhattacharyya, Asmita Samaddar, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Anticancer potentials of root extract of Polygala senega against benzo[a]pyrene-induced lung cancer in mice. Journal of Chinese Integrative Medicine, 2011, 9(3): 320-327.
[14] Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Boujedaini N. Polymeric nanoparticle encapsulation of a naturally occurring plant scopoletin and its effects on human melanoma cell A375. Journal of Chinese Integrative Medicine, 2010, 8(9): 853-862.
[15] Wen Sun, Jian Yu, Yu-min Shi, Hao Zhang, Ying Wang, Bing-bing Wu. Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage. Journal of Chinese Integrative Medicine, 2010, 8(4): 341-346.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[4] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[5] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[6] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[7] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[8] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[9] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[10] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079