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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 974-978.doi: 10.3736/jcim20101010

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Bushen Shugan Recipe in regulating the hypothalamus-pituitary-ovarian axis in a rat model of stress-induced anorexia

WX Hu,CQ Yu,DX Zhai,J Han,DY Zhang   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2010-05-16 Accepted:2010-08-31 Online:2010-10-20 Published:2010-10-15


To investigate the effects of Bushen Shugan Recipe (BSSGR), a compound traditional Chinese herbal medicine, in regulating the hypothalamus-pituitary-ovarian axis (HPOA) in a rat model of stress-induced anorexia.

Anorexia was induced in rats by the methods of separation, diet restriction and constraint. Rats were divided into 4 groups randomly: control group, untreated group, sham-operated group and BSSGR group. After the experiments, body weights and oestrous cycles of the 4 groups were compared. The levels of serum estradiol (E2), hypophysis luteotrophic hormone (LH), hypophysis follicle stimulating hormone (FSH) and hypothalamus β-endorphin (β-EP) were detected by radioimmunoassay. The level of serum corticosterone (CORT) was detected by enzyme-linked immunosorbent assay.


Body weight of BSSGR group was significantly increased in comparison with sham-operated group(P<0.01); the oestrous cycle disordering rate was higher than those of the untreated group and sham-operated group; hypophysis LH and serum E2 were obviously increased in comparison with untreated group (P<0.05); hypothalamus β-EP was obviously decreased in comparison with sham-operated group (P<0.05); serum CORT was obviously decreased in comparison with untreated group (P<0.05), and significantly decreased in comparison with sham-operated group (P<0.01).

BSSGR increased hypophysis LH and serum E2, and decreased serum CORT and hypothalamus β-EP in rats with stress-induced anorexia.

Key words: Compounds(TCD), Anorexia nervosa, Stress models, Hypothalamus-pituitary-ovarian axis

Table 1

Body weights of rats in different groups"

Table 2

Levels of hypophysis FSH and LH and serum E2 of rats in different groups"

Table 3

Levels of hypothalamus β-EP and serum CORT of rats in different groups"

[1] Zhao P, Mao FQ, Li ZT . Investigation of clinical feature and psychiatric intervention of anorexia nervosa[J]. Guo Ji Zhong Hua Shen Jing Jing Shen Yi Xue Za Zhi, 2005,6(3):201-203
赵朋, 毛富强, 李振涛 . 神经性厌食症的临床特点及精神科干预对策[J]. 国际中华神经精神医学杂志, 2005,6(3):201-203
[2] Liu CH, Dou JT, Lu JM . The changes of endocrine function in patients with anorexia nervosa[J]. Lin Chuang Nei Ke Za Zhi, 2002,19(6):449-450
doi: 10.3969/j.issn.1001-9057.2002.06.018
吕朝晖, 窦京涛, 陆菊明 . 神经性厌食患者内分泌功能的改变[J]. 临床内科杂志, 2002,19(6):449-450
doi: 10.3969/j.issn.1001-9057.2002.06.018
[3] Shimizu N, Oomura Y, Kai Y . Stress-induced anorexia in rats mediated by serotonergic mechanisms in the hypothalamus[J]. Physiol Behav, 1989,46(5):835-841
doi: 10.1016/0031-9384(89)90045-0 pmid: 2628995
[4] Wang ZZ, Yu CQ . Effects of Bushen Huayu Qutan Recipe on local ovarian factors in androgen-sterilized rats[J]. J Chin Integr Med, 2008,6(4):361-365
王针织, 俞超芹 . 补肾化瘀祛痰方对雄激素致不孕大鼠卵巢局部调控因子的影响[J]. 中西医结合学报, 2008,6(4):361-365
[5] Zhang S, Wang X, Wei XB, Tan JH . Observation on the vaginal smear photographs for each stage of estrus cycle in rats[J]. Dong Wu Yi Xue Jin Zhan, 2006,27(2):69-72
doi: 10.3969/j.issn.1007-5038.2006.02.018
张森, 王新, 韦旭斌, 谭建华 . 大鼠发情周期各阶段的阴道细胞变化观察[J]. 动物医学进展, 2006,27(2):69-72
doi: 10.3969/j.issn.1007-5038.2006.02.018
[6] Wang JH, Wang MZ, Ouyang D, Wu QH . Effects of flavonoids from Semen Cuscutae on changes of β-EP in hypothalamuses and FSH and LH in anterior pituitaries in female rats exposed to psychologic stress[J]. Zhong Yao Cai, 2002,25(12):886-888
doi: 10.3321/j.issn:1001-4454.2002.12.015
王建红, 王敏璋, 欧阳栋, 伍庆华 . 菟丝子黄酮对心理应激雌性大鼠下丘脑β-EP与腺垂体FSH、LH的影响[J]. 中药材, 2002,25(12):886-888
doi: 10.3321/j.issn:1001-4454.2002.12.015
[7] Yen SS, Jaffe RB, Barbieri RL. Reproductive Endocrinology[M]. Beijing: Science Press,2001: 546-550
Yen SS, Jaffe RB, Barbieri RL. Reproductive Endocrinology[M]. 北京: 科学出版社,2001: 546-550
[8] Yang JB, Li XH . On the relationship between β-endorphin and female genital endocrine[J]. Shanxi Zhong Yi Xue Yuan Xue Bao, 2004,27(3):9-11
杨鉴冰, 李小花 . β-内啡肽与女性生殖内分泌的关系探讨[J]. 陕西中医学院学报, 2004,27(3):9-11
[9] Hou ZM, Wang YR . Application of tonifying kidney in gynecology[J]. Nei Meng Gu Zhong Yi Yao, 2008,( 2):20-21
doi: 10.3969/j.issn.1006-0979.2008.02.016
侯志明, 王艳荣 . 补肾法在妇科中的运用[J]. 内蒙古中医药, 2008,( 2):20-21
doi: 10.3969/j.issn.1006-0979.2008.02.016
[10] Ge JL . Examples of soothing liver in gynecology[J]. Jiangsu Zhong Yi Yao, 2005,26(10):33-34
doi: 10.3969/j.issn.1672-397X.2005.10.019
阁继兰 . 疏肝论治妇科病举隅[J]. 江苏中医药, 2005,26(10):33-34
doi: 10.3969/j.issn.1672-397X.2005.10.019
[11] Saketos M, Sharma N, Santoro NF . Suppression of the hypothalamic-pituitary-ovarian axis in normal women by glucocorticoids[J]. Biol Reprod, 1993,49(6):1270-1276
doi: 10.1095/biolreprod49.6.1270
[12] Breen KM, Billings HJ, Wagenmaker ER, Wessinger EW, Karsch FJ . Endocrine basis for disruptive effects of cortisol on preovulatory events[J]. Endocrinology, 2005,146(4):2107-2115
doi: 10.1210/en.2004-1457 pmid: 15625239
[13] Breen KM, Karsch FJ . Does cortisol inhibit pulsatile luteinizing hormone secretion at the hypothalamic or pituitary level?[J]. Endocrinology, 2004,145(2):692-698
doi: 10.1210/en.2003-1114
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