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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (4): 435-441.doi: 10.3736/jcim20110413

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Chinese herbal medicine Compound Zhuye Shigao Granule in rats with radiation-induced esophagitis

Jun-zhang Lu1(), Li Zhao1, Jian-ping Ren2, Xiu-tang Cao3, Hai-xia Li4, Hong Zhao1   

  1. 1. Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China
    2. Institute of Irradiation and Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
    3. Department of Medical Statistics, Chinese PLA General Hospital, Beijing 100853, China
    4. Department of Internal Medicine, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2011-01-18 Accepted:2011-03-14 Online:2011-04-20 Published:2011-04-15
  • Contact: Lu Jun-zhang E-mail:lujunzhang@sina.com

Objective: To explore the preventive and therapeutic effects of Compound Zhuye Shigao Granule (CZSG), a compound Chinese herbal medicine, on radiation-induced esophagitis in rats.
Methods: Fifty-six Wistar rats were randomly divided into 5 groups: normal control group (8 rats), model group (12 rats), and high-, medium- and low-dose CZSG groups (12 rats in each group). The rats in the normal control and model groups were given normal saline 10 mL/kg body weight and those in the CZSG-treated groups were given solution of CZSG at doses of 1.15, 2.30, or 4.60 g/kg body weight respectively by intragastric administration once a day for 7 days. Then esophagitis was induced by local irradiation of 60Co ray (490.25 cGy/min, totaled 30 Gy). The administration was continued for another 7 days or 14 days, meanwhile body weight and daily food intake of the rats were recorded. Seven days after the irradiation, 4 rats in each group were sacrificed under anesthesia, then, the esophageal tissue was obtained for histopathological examination and the degrees of esophageal tissue injury and neutrophil infiltration were scored. Fourteen days after the irradiation, all the survival rats were dealt in the same way.
Results: (1) Seven days after the irradiation, the esophageal tissue sections in the model group showed typical histopathological changes of radiation-induced esophagitis, whereas in the CZSG groups the histopathological changes were lessened dose-dependently and in the high-dose CZSG group the esophageal tissue remained basically intact. (2) The scores of esophageal tissue injury and cellular infiltration in the high- and medium-dose CZSG groups were both significantly less than in the model group (P<0.05). (3) The body weight of the rats increased in the normal control group, whereas it decreased obviously in the model group. In the medium- and high-dose CZSG groups, it did not decrease significantly. The decrease of body weight in the high-dose CZSG group was less than that in the model group (P<0.05). (4) Compared with the normal control group, the daily food intake was reduced in the other groups. However, it was significantly greater in the low, medium- and high-dose CZSG groups than in the model group (P<0.05).
Conclusion: In rats with radiation-induced esophagitis, CZSG can effectively relieve the esophageal tissue injury and cellular infiltration, increase daily food intake, and prevent rats from lose of body weight dose-dependently, which show that CZSG has the preventive and therapeutic effects for radiation-induced esophagitis in rats.

Key words: compound (TCD), radiation injuries, experimental, esophagitis, therapeutic methods (TCM), rats

Figure 1

Pathological changes of esophageal tissue 7 d after irradiation (Hematoxylin-eosin staining, light microscopy, ×10) A: Model group; B: Low-dose Compound Zhuye Shigao Granule group; C: Medium-dose Compound Zhuye Shigao Granule group; D: High-dose Compound Zhuye Shigao Granule group."

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Figure 2

Body weight of rats in each group CZSG: Compound Zhuye Shigao Granule."

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Figure 3

Average daily food intake of rats in each group CZSG: Compound Zhuye Shigao Granule."

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