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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (4): 435-441.doi: 10.3736/jcim20110413

• Original Experimental Research • Previous Articles     Next Articles

Preventive and therapeutic effects of Chinese herbal medicine Compound Zhuye Shigao Granule in rats with radiation-induced esophagitis

Jun-zhang Lu1(), Li Zhao1, Jian-ping Ren2, Xiu-tang Cao3, Hai-xia Li4, Hong Zhao1   

  1. 1. Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China
    2. Institute of Irradiation and Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
    3. Department of Medical Statistics, Chinese PLA General Hospital, Beijing 100853, China
    4. Department of Internal Medicine, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2011-01-18 Accepted:2011-03-14 Online:2011-04-20 Published:2011-04-15
  • Contact: Lu Jun-zhang

Objective: To explore the preventive and therapeutic effects of Compound Zhuye Shigao Granule (CZSG), a compound Chinese herbal medicine, on radiation-induced esophagitis in rats.
Methods: Fifty-six Wistar rats were randomly divided into 5 groups: normal control group (8 rats), model group (12 rats), and high-, medium- and low-dose CZSG groups (12 rats in each group). The rats in the normal control and model groups were given normal saline 10 mL/kg body weight and those in the CZSG-treated groups were given solution of CZSG at doses of 1.15, 2.30, or 4.60 g/kg body weight respectively by intragastric administration once a day for 7 days. Then esophagitis was induced by local irradiation of 60Co ray (490.25 cGy/min, totaled 30 Gy). The administration was continued for another 7 days or 14 days, meanwhile body weight and daily food intake of the rats were recorded. Seven days after the irradiation, 4 rats in each group were sacrificed under anesthesia, then, the esophageal tissue was obtained for histopathological examination and the degrees of esophageal tissue injury and neutrophil infiltration were scored. Fourteen days after the irradiation, all the survival rats were dealt in the same way.
Results: (1) Seven days after the irradiation, the esophageal tissue sections in the model group showed typical histopathological changes of radiation-induced esophagitis, whereas in the CZSG groups the histopathological changes were lessened dose-dependently and in the high-dose CZSG group the esophageal tissue remained basically intact. (2) The scores of esophageal tissue injury and cellular infiltration in the high- and medium-dose CZSG groups were both significantly less than in the model group (P<0.05). (3) The body weight of the rats increased in the normal control group, whereas it decreased obviously in the model group. In the medium- and high-dose CZSG groups, it did not decrease significantly. The decrease of body weight in the high-dose CZSG group was less than that in the model group (P<0.05). (4) Compared with the normal control group, the daily food intake was reduced in the other groups. However, it was significantly greater in the low, medium- and high-dose CZSG groups than in the model group (P<0.05).
Conclusion: In rats with radiation-induced esophagitis, CZSG can effectively relieve the esophageal tissue injury and cellular infiltration, increase daily food intake, and prevent rats from lose of body weight dose-dependently, which show that CZSG has the preventive and therapeutic effects for radiation-induced esophagitis in rats.

Key words: compound (TCD), radiation injuries, experimental, esophagitis, therapeutic methods (TCM), rats

Figure 1

Pathological changes of esophageal tissue 7 d after irradiation (Hematoxylin-eosin staining, light microscopy, ×10) A: Model group; B: Low-dose Compound Zhuye Shigao Granule group; C: Medium-dose Compound Zhuye Shigao Granule group; D: High-dose Compound Zhuye Shigao Granule group."



Figure 2

Body weight of rats in each group CZSG: Compound Zhuye Shigao Granule."


Figure 3

Average daily food intake of rats in each group CZSG: Compound Zhuye Shigao Granule."

[1] Shen WJ, Wang LH. Injury of radiotherapy[M]. Beijing: China Press of Traditional Chinese Medicine, 2001: 146
申文江, 王绿化 . 放射治疗损伤[M]. 北京: 中国医药科技出版社, 2001: 146
[2] Lu JZ, Wang FW, Cui SX, Li HX, Hu J, Fang P, Liu ZF . Clinical study on Zhuye Shigao decoction in preventing and treating radiation esophagitis[J]. Zhong Yi Za Zhi, 2000,41(5):293-294
doi: 10.3321/j.issn:1001-1668.2000.05.022
路军章, 王发渭, 崔书祥, 李海霞, 呼健, 方平, 刘哲峰 . 竹叶石膏汤防治放射性食管炎的临床观察[J]. 中医杂志, 2000,41(5):293-294
doi: 10.3321/j.issn:1001-1668.2000.05.022
[3] Lu JZ, Zhao H, Liu Y, Feng LC . Clinical study on prevention and treatment for acute radiation esophagitis by Modified Lophatheri and Gypsi Fibrosi Decoction[J]. Zhonghua Zhong Yi Yao Za Zhi, 2010,25(1):59-62
路军章, 赵红, 刘毅, 冯林春 . 加味竹叶石膏汤防治急性放射性食管炎的临床研究[J]. 中华中医药杂志, 2010,25(1):59-62
[4] Trowers E, Thomas C Jr, Silverstein FE . Chemical and radiation induced esophageal injury[J]. Gastrointest Endosc Clin North Am, 1994,4(4):657-675
doi: 10.1016/S1052-5157(18)30474-4 pmid: 7812640
[5] Liao Z, Cox JD, Komaki R . Radiochemotherapy of esophageal cancer[J]. J Thorac Oncol, 2007,2(6):53-68
doi: 10.1097/01.JTO.0000275339.62831.5e pmid: 17545853
[6] Bradley J, Movsas B . Radiation pneumonitis and esophagitis in thoracic irradiation[J]. Cancer Treat Res, 2006,128:43-64
doi: 10.1007/b107220
[7] Gong YJ, Zhang L, Zhang X . Effect of Compound Shaogen Mixture for the treatment of acute radiation-induced esophagitis: an observation of 18 cases[J]. Xin Zhong Yi, 2008,40(2):39-40
宫彦军, 张莉, 张辛 . 复方芍根合剂治疗放射性食管炎18例疗效观察[J]. 新中医, 2008,40(2):39-40
[8] Fang P, Wang FW, Cui SX, Jiao SC . Clinical study on the effect of Yangyinjiandu decoction on acute radiation esophagitis[J]. Zhongguo Fang She Zhong Liu Xue Za Zhi, 2005,14(5):404-406
doi: 10.3760/j.issn:1004-4221.2005.05.007
方平, 王发渭, 崔书祥, 焦顺昌 . 养阴减毒汤防治放射性食管炎的疗效观察[J]. 中华放射肿瘤学杂志, 2005,14(5):404-406
doi: 10.3760/j.issn:1004-4221.2005.05.007
[9] Yao WH . Clinical study on the effect of Shashen Maidong decoction on acute radiation esophagitis[J]. Zhonghua Fang She Yi Xue Yu Fang Hu Za Zhi, 2004,24(4):376
姚卫华 . 沙参麦门冬汤加味防治放射性食管炎疗效观察[J]. 中华放射医学与防护杂志, 2004,24(4):376
[10] Huang ZF, Liu JB, Li HZ, Zhang ZJ, Tan ZQ, Chen C . Radiation esophagitis treated by Xuefuzhuyu decoction: a report of 32 cases[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2006,14(3):200-201
黄智芬, 刘俊波, 黎汉忠, 张作军, 谭志强, 陈闯 . 血府逐瘀汤治疗放射性食管炎32例[J]. 中国中西医结合消化杂志, 2006,14(3):200-201
[11] Duan YL, Fan XH, Shi ZZ, Sun CX, Liu LP, Liu GL . Effect of Xuejie in prevention and treatment of radiation esophagitis[J]. Zhonghua Fang She Yi Xue Yu Fang Hu Za Zhi, 2006,26(5):459
doi: 10.3760/cma.j.issn.0254-5098.2006.05.040
段玉龙, 范向辉, 史中州, 孙朝选, 刘利平, 刘桂玲 . 单味血竭对放射性食管炎的防治作用[J]. 中华放射医学与防护杂志, 2006,26(5):459
doi: 10.3760/cma.j.issn.0254-5098.2006.05.040
[12] Li AM, Dong X, Chen SH, Zhao XY, Meng QP . Clinical study on White Drug powder mixture used to prevent and treat radiation esophagitis[J]. Zhongguo Zhong Liu Lin Chuang Yu Kang Fu, 2006,13(1):89-91
doi: 10.3969/j.issn.1005-8664.2006.01.038
李爱梅, 董霞, 陈生宏, 赵晓燕, 孟庆萍 . 云南白药合剂防治放射性食管炎的临床观察[J]. 中国肿瘤临床与康复, 2006,13(1):89-91
doi: 10.3969/j.issn.1005-8664.2006.01.038
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