Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 821-826.doi: 10.3736/jcim20080810

• Original Experimental Research • Previous Articles     Next Articles

Effects of Scutellaria Barbata drug-containing serum on apoptosis and mitochondrial transmembrane potential of hepatoma H22 cells

Zhi-jun Dai1(), Xi-jing Wang1, Qian Xue2, Zong-zheng Ji3, Xiao-xu Liu1, Hua-feng Kang1, Hai-tao Guan1, Xiao-bin Ma1, Hong-tao Ren1   

  1. 1. Department of Oncology, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University,Xi'an,Shaanxi Province 710004, China
    2. Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China
    3. Department of General Surgery, The Second Affiliated Hospital of Medical College, Xi’an Jiaotong University,Xi’an, Shaanxi Province 710004, China;
  • Received:2007-11-29 Online:2008-08-20 Published:2008-08-15

Objective: To investigate the effects of serum containing Scutellaria Barbata extract (ESB) on apoptosis rate and mitochondrial transmembrane potential (MTP) of liver cancer cell line H22 from mice in vitro.
Methods: H22 cells were cultured in vitro and divided into 5 groups: blank control group, low-dose ESB group, medium-dose ESB group, high-dose ESB group and fluorouracil (5-Fu) group. Methyl thiazolyl tetrazolium assay was utilized to determine the proliferation rates of H22 cells. Cellular morphology was observed under a transmission electron microscope (EM). The rhodamine 123 was used as a fluorescence probe to label the H22 cells, and the fluorescence intensities were observed with a laser scanning confocal microscope. The fluorescence intensity of H22 cells indicated the MTP of H22 cells.
Results: The inhibition of serum containing ESB on the proliferation of H22 cells in vitro was observerd in a time-dependent manner. The typical morphological changes of apoptosis were observed after incubation with ESB-containing serum in high dose for 48h. The apoptosis rate of blank control group, 5-Fu group, low-dose ESB group, medium-dose ESB group and high-dose ESB group were (0.51±0.32)%, (11.26±2.97)%, (1.07±0.46)%, (3.15±1.12)%, (7.83±2.25)% respectively. ESB could reduce the MTP of H22 cells from mice as compared with those in the untreated group. The MTPs of the blank control group, ESB in low dose, medium dose, and high dose groups and 5-Fu group were (245.45±67.37), (127.42±41.35), (213.68±65.52), (186.34±56.37) and (142.65±39.44) respectively, which was negatively correlated with apoptosis rate.
Conclusion: ESB-containing serum effectively induces apoptosis, which may be related to the decrease of MTP in H22 cells.

Key words: Scutellaria barbata, liver cancer, apoptosis, mitochondrial transmembrane potential, drug- containing serum, in vitro

CLC Number: 

  • R285.5

Figure 1

Growth inhibiting effects of ESB-containing serum on H22 cell proliferation"

Figure 2

Morphological change of H22 cells after 48-hour treatment by high-dose ESB-containing serum (transmission electron microscopy, ×5 000) A: Normal H22 cells; B: Karyopyknosis and chromatic agglutination; C: Apoptotic body."

Figure 3

Effect of ESB-containing serum on cell cycle ratios and apoptosis rates of H22 cells A: Blank control group; B: 5-Fu group; C: Low-dose ESB group; D: Medium-dose ESB group; E: High-dose ESB group."

Figure 4

Effect of ESB-containing serum on MTP of H22 cells A: Blank control group; B: 5-Fu group; C: Low-dose ESB group; D: Medium-dose ESB group; E: High-dose ESB group."

[1] Yin X, Zhou J, Jie C , et al. Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549[J]. Life Sci, 2004,75(18):2233-2244
doi: 10.1016/j.lfs.2004.05.015
[2] Ye J, Xu XK, Zhou JW , et al. Prevention and treatment of herba Scutellariae barbatae compound on diethylnitrosamine-induced hepatocarcinoma of rat[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2002,10(2):67-70
doi: 10.3969/j.issn.1671-038X.2002.02.001
叶健, 徐锡坤, 周建伟 , 等. 复方半枝莲防治二乙基亚硝胺诱发大鼠肝癌的研究[J]. 中国中西医结合消化杂志, 2002,10(2):67-70
doi: 10.3969/j.issn.1671-038X.2002.02.001
[3] Lin JM, Liu Y, Luo RC . Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells[J]. Nan Fang Yi Ke Da Xue Xue Bao, 2006,26(5):591-593
doi: 10.3321/j.issn:1673-4254.2006.05.014
林敬明, 刘煜, 罗荣城 . 半枝莲抑制人肝癌QGY-7701细胞增殖研究[J]. 南方医科大学学报, 2006,26(5):591-593
doi: 10.3321/j.issn:1673-4254.2006.05.014
[4] Mohamad N, Gutiérrez A, Núñez M , et al.Mitochondrial apoptotic pathways[J]. Biocell, 2005,29(2):149-161
[5] Blattner JR, He L, Lemasters JJ . Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader[J]. Anal Biochem, 2001,295(2):220-226
doi: 10.1006/abio.2001.5219
[6] Wang WS, Zhou YW, Ye YH , et al. Studies on the flavonoids in herb from Scutellaria barbata[J]. Zhongguo Zhong Yao Za Zhi, 2004,29(10):957-959
doi: 10.3321/j.issn:1001-5302.2004.10.009
王文蜀, 周亚伟, 叶蕴华 , 等. 半枝莲中黄酮类化学成分研究[J]. 中国中药杂志, 2004,29(10):957-959
doi: 10.3321/j.issn:1001-5302.2004.10.009
[7] Wang G, Dong M, Liu XS , et al. Anti-tumor activity study of extract from Scutellaria barbata D. Don[J]. Xian Dai Zhong Xi Yi Jie He Za Zhi, 2004,13(9):1141-1142
doi: 10.3969/j.issn.1008-8849.2004.09.010
王刚, 董玫, 刘秀书 , 等. 半枝莲醇提物抗肿瘤活性的研究[J]. 现代中西医结合杂志, 2004,13(9):1141-1142
doi: 10.3969/j.issn.1008-8849.2004.09.010
[8] Xie LK, Deng T, Zhang QP , et al. Apoptosis of leukemia K562 cells induced by Scutellaria barbata extract[J]. Wuhan Da Xue Xue Bao Yi Xue Ban, 2004,25(2):115-117
谢珞琨, 邓涛, 张秋萍 , 等. 半枝莲提取物诱导白血病K562 细胞凋亡[J]. 武汉大学学报(医学版), 2004,25(2):115-117
[9] Gao D, Gao YL, Bai P . Influence of Scutellaria balbata on calcium beaconage of cervix cancer cells[J]. Zhong Yao Cai, 2003,26(10):730-733
doi: 10.3321/j.issn:1001-4454.2003.10.019
高冬, 高永琳, 白平 . 半枝莲对宫颈癌细胞钙信号系统影响[J]. 中药材, 2003,26(10):730-733
doi: 10.3321/j.issn:1001-4454.2003.10.019
[10] Han KQ, Ling CQ . Current situation and outlook of serum pharmacology reseach in anti-cancer effect of traditional Chinese medicine in vitro[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2003,23(9):717-719
doi: 10.7661/CJIM.2003.9.717
韩克起, 凌昌全 . 中药体外抗肿瘤效应血清药理学研究现状与前景[J]. 中国中西医结合杂志, 2003,23(9):717-719
doi: 10.7661/CJIM.2003.9.717
[11] Zhao JX, Guo FL, Bai DC , et al. Effects of Fuzheng Yiliu Granules on apoptotic rate and mitochondrial membrane potential of hepatocellular carcinoma cell line H22 from mice[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(3):271-274
赵健雄, 郭芙莲, 白德成 , 等. 扶正抑瘤颗粒对小鼠肝癌H22细胞凋亡率及线粒体膜电位的影响[J]. 中西医结合学报, 2006,4(3):271-274
[12] Lucken-Ardjomande S, Martinou JC . Newcomers in the process of mitochondrial permeabilization[J]. J Cell Sci, 2005,118(Pt 3):473-483
doi: 10.1242/jcs.01654 pmid: 15673686
[1] Chang-quan Ling, Jia Fan, Hong-sheng Lin, Feng Shen, Zhen-ye Xu, Li-zhu Lin, Shu-kui Qin, Wei-ping Zhou, Xiao-feng Zhai, Bai Li, Qing-hui Zhou, on behalf of the Chinese Integrative Therapy of Primary Liver Cancer Working Group. Clinical practice guidelines for the treatment of primary liver cancer with integrative traditional Chinese and Western medicine. Journal of Integrative Medicine, 2018, 16(4): 236-248.
[2] Jong-Min Kim, Kyung-Soon Kim, Yeon-Weol Lee, Chong-Kwan Cho, Hwa-Seung Yoo, Ji-Young Bang, Eung-Yoon Kim, In-Cheol Kang. Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis. Journal of Chinese Integrative Medicine, 2011, 9(9): 1005-1013.
[3] Bao Tan, Hai-lian Shi, Guang Ji, Jian-qun Xie. Effects of taraxerol and taraxeryl acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS. Journal of Chinese Integrative Medicine, 2011, 9(6): 638-642.
[4] Zhao-hui Chu, Xiao-hua Liang, Xin-li Zhou, Ruo-fan Huang, Qiong Zhan, Jing-wei Jiang. Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway. Journal of Chinese Integrative Medicine, 2011, 9(5): 533-538.
[5] Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Boujedaini N. Polymeric nanoparticle encapsulation of a naturally occurring plant scopoletin and its effects on human melanoma cell A375. Journal of Chinese Integrative Medicine, 2010, 8(9): 853-862.
[6] Ju-yong Wang, Zhan Zheng, Zhen-ye Xu, Jing Guo, Wan So. Effects of Feiyanning Decoction on proliferation of lung adenocarcinoma cell line and their production of interleukin-6 and interleukin-8 induced by tumor necrosis factor-α. Journal of Chinese Integrative Medicine, 2009, 7(8): 741-745.
[7] Huo-jun Zhang, Ji-jin Yang, Wei-xing Wang, Xu Jiang, Yan-jun Mao, Chao-ai Yang, Ji-xiang Guo. Effects of Jinlong Capsule on expressions of interleukin-2 and soluble interleukin-2 receptor in patients with primary liver cancer after transarterial chemoembolization therapy. Journal of Chinese Integrative Medicine, 2008, 6(9): 907-910.
[8] Zhi-qiang Pan, Zhao-qin Fang, Wen-li Lu, Chao Liang, Zhong-hua Wu, Xiao-mei Liu, Li Hou, Hui Zhang, Shao-yuan Zhuo, Ming-juan Liao, Bi-feng Gao. Differentially expressed genes in adrenal gland of H22 liver cancer mice with different syndromes and in different stages. Journal of Chinese Integrative Medicine, 2008, 6(8): 843-851.
[9] Zhi-jun Dai, Xi-jing Wang, Zong-zheng Ji, Zong-fang Li, Wei Tang, Hua-feng Kang, Xiao-bin Ma, Lei Liu. Scutellaria barbata extract enhances efficacy and reduces toxicity of chemotherapy in hepatoma H22-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(7): 720-724.
[10] Hong-yun Chen, Zhe Chen. Transcatheter arterial chemoembolization combined with other therapies for the treatment of primary hepatic carcinoma: a review. Journal of Chinese Integrative Medicine, 2008, 6(4): 422-426.
[11] Hai-bo Yu, Cheng-en Pan, Wu-jun Wu, Si-hai Zhao, Hui-feng Zhang. Effects of resveratrol on matrix metalloproteinase-9 expression in hepatoma cells. Journal of Chinese Integrative Medicine, 2008, 6(3): 270-273.
[12] Jia Guo, Dong Li, Qiu-fang Zhang. Acupuncture intervention combined with assisted reproductive technology: its different effects at different time points during the in vitro fertilization-embryo transfer course. Journal of Chinese Integrative Medicine, 2008, 6(12): 1211-1216.
[13] Zhen Sun, Yong-hua Su, Xiao-qiang Yue. Professor Ling Changquan's experience in treating primary liver cancer: an analysis of herbal medication. Journal of Chinese Integrative Medicine, 2008, 6(12): 1221-1225.
[14] Tan Li, Wei Wang, Tong Li. Antileukemic mechanism of resveratrol in vitro and in mice bearing L1210-tumor. Journal of Chinese Integrative Medicine, 2008, 6(12): 1267-1274.
[15] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50.
Full text



[1] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[5] Ya-hui Liang , Ping Li, Jing-xia Zhao, Xin Liu, Qi-fu Huang. Acetyl-11-keto-beta-boswellic acid and arsenic trioxide regulate the productions and activities of matrix metalloproteinases in human skin fibroblasts and human leukemia cell line THP-1. Journal of Chinese Integrative Medicine, 2010, 8(11): 1060-1069
[6] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[7] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[8] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Liang-ping Hu, Xiao-lei Bao. Three-factor designs unable to examine the interactions (Part 2). Journal of Chinese Integrative Medicine, 2012, 10(11): 1229-1232