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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (7): 746-751.doi: 10.3736/jcim20110708

• Original Experimental Research • Previous Articles     Next Articles

Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats

Tapas Kumar Sur, Biswajit Auddy, Dipankar Bhattacharyya()   

  1. Department of Pharmacology, Institute of Postgraduate Medical Education and Research, West Bengal University of Health Sciences,Kolkata 700020, West Bengal, India
    Department of Pharmacology, Institute of Postgraduate Medical Education and Research, West Bengal University of Health Sciences, Kolkata 700020, West Bengal, India
  • Received:2011-02-15 Accepted:2011-04-06 Online:2011-07-20 Published:2011-06-15
  • Supported by:
    M/s Health Reactive (Baddi, Solan, Himachal Pradesh, India) provid the research grant.

Objective: To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human platelet aggregation and isoproterenol (IS)-induced myocardial ischemia (MI) damage in male Wistar rats.
Methods: BR suspension 10, 20 and 30 μg was mixed with platelet-rich plasma and incubated at 37 ℃ for 30 min, respectively. Then, adenosine diphosphate (ADP, 20 mmol/L) or collagen (2 μg) was added in the mixture and the aggregation was observed against platelet-poor plasma mixed with equal volume of suspension of the same test samples. Wistar rats divided into 4 groups were used to investigate BR’s effects on IS-induced MI. Levels of serum creatinine kinase (CK), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated by standard commercial biological kits. Serum nitric oxide (NOx) was also measured. The lipid peroxides (LPO) and protein concentrations in heart tissues were measured.
Results: BR could inhibit ADP- or collagen-induced human platelet aggregation dose-dependently. Moreover, it could protect MI caused by IS in rats. BR reduced the levels of serum CK, AST, ALT and NOx dose-dependently and also lowered LPO in heart tissues in comparison with the MI control (P<0.01).
Conclusion: BR can inhibit human platelet aggregation and protect MI caused by IS in rats.

Key words: platelet aggregation inhibitors, myocardial ischemia, lipid peroxidation, nitric oxide, plant extracts, rats

"

Group n ADP-induced platelet
aggregation (%)
ADP control (ADP 20 mmol/L) 6 86.23±0.51
BR 10 μg 6 65.66±0.23**
BR 20 μg 6 51.41±0.45**
BR 30 μg 6 46.53±0.48**

"

Group n Collagen-induced platelet
aggregation (%)
Collagen control (collagen 2 μg) 6 72.10±0.49
BR 10 μg 6 59.68±0.38△△
BR 20 μg 6 48.37±0.31△△
BR 30 μg 6 44.51±0.28△△

"

Group n Serum level Heart tissue
LPO (nmol/L)
CK (U/L) AST (U/L) ALT (U/L) NOx (μmol/L)
Normal control 6 88.10±3.69 38.01±1.23 46.50±1.28 13.83±1.22 0.71±0.02
Ischemic model control (IS 85 mg/kg) 6 268.50±8.28▲▲ 90.65±2.57▲▲ 106.62±2.38▲▲ 40.83±1.01▲▲ 2.16±0.09▲▲
IS (85 mg/kg)+BR (200 mg/kg) 6 193.50±4.42□□ 62.16±1.47□□ 82.15±3.02□□ 28.82±0.60□□ 1.54±0.03□□
IS (85 mg/kg)+BR (400 mg/kg) 6 159.65±5.76□□ 50.51±1.25□□ 69.84±1.70□□ 24.31±0.88□□ 1.29±0.06□□
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