红花注射液对急性冠状动脉综合征患者血小板糖蛋白Ⅱb/Ⅲa受体影响的随机对照研究

doi:10.3736/jcim20120311

Abstract

BACKGROUND: Glycoprotein (GP) Ⅱb/Ⅲa is an important index for assessing the function of platelets. To investigate the effects of Honghua Injection, a Chinese patent medicine made from extracts of Carthamus tinctorius L, on GP Ⅱb/Ⅲa is a key study in evaluating the inhibition properties of Honghua Injection on platelet aggregation.
OBJECTIVE: To observe the effects of Honghua Injection on platelet GPⅡb/Ⅲa receptors and explore the mechanisms of Honghua Injection in inhibiting platelet aggregation in patients with acute coronary syndrome.
DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: A total of 64 patients from Yueyang Hospital of Traditional Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine were randomly divided into Honghua Injection group (n=32, routine Western medicine plus Honghua Injection) and control group (n=32, routine Western medicine) according to the random number table. The patients were treated for 14 d.
MAIN OUTCOME MEASURES: The expressions of GPⅡb/Ⅲa (CD41), P-selection (CD62p), and lysosome-associated membrane GP (CD63) were measured by flow cytometry before and after treatment. Meanwhile the therapeutic effects of Huonghua Injection on angina and short-term prognosis were observed.
RESULTS: The observational period was 14 d and four patients in the control group were omitted due to early discharge. The expression of CD41 in both the Honghua Injection group and the control group was inhibited after treatment (P<0.05, P<0.01). The inhibition of the Honghua Injection group was stronger than that of the control group (P<0.05). There was no case of death, myocardial infarction and cerebral apoplexy during 14 d of treatment. The improvement of angina symptoms and electrocardiographic manifestation in the Honghua Injection group was greater than that of the control group (P<0.05).
CONCLUSION: Honghua Injection can inhibit the expression of GPⅡb/Ⅲa receptors by preventing aggregation of platelets and may be considered as an effective traditional Chinese medicine for acute coronary syndrome.

Key words: coronary disease, acute coronary syndrome, Chinese patent drugs, platelet membrane glycoprotein, platelet glycoprotein GPⅡb-Ⅲa complex;, P-selectin, randomized controlled trial

Zhu Yan-feng, Luo Hai-ming, Deng Zhong-long, Fu De-yu, Yao Wang, Dai Jian. Effects of the Chinese patent medicine, Honghua Injection, on platelet glycoprotein Ⅱb/Ⅲa receptors in patients with acute coronary syndrome: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(3): 318-323.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20120311

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References 13

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Item	Control group (n=28)	Treatment group (n=32)
Age (x±s, years)	72.07±10.27	69.56±11.46
Gender (male/female)	22/6	23/9
Acute myocardial infarction	14	13
Elective PCI	12	14
CABG	2	1

Group	n	CD41 (%)	Fluorescence value of CD41	CD62p (%)	CD63 (%)
Control
Before treatment	28	96.12±4.10	12.01±2.69	11.16±4.27	10.82±3.74
After treatment	28	93.41±4.19^*	10.36±2.63^*	10.89±4.75	10.09±3.56
Treatment
Before treatment	32	96.03±3.17	12.54±3.12	10.59±4.96	11.00±4.45
After treatment	32	90.85±4.53^**△	8.85±2.91^**△	10.55±4.70	10.26±4.30

Group	n	Obvious effective	Improve	Not effective	Aggravated
Control	28	14 (50.0)	10 (35.7)	4 (14.3)	0 (0)
Treatment	32	23 (71.9)	7 (21.9)	2 (6.2)	0 (0)

Group	n	Obvious effective	Improve	Not effective	Aggravated
Control	28	12 (42.9)	9 (32.1)	7 (25)	0 (0)
Treatment	32	21 (65.6)	8 (25.0)	3 (9.4)	0 (0)

Effects of the Chinese patent medicine, Honghua Injection, on platelet glycoprotein Ⅱb/Ⅲa receptors in patients with acute coronary syndrome: a randomized controlled trial

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