Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (3): 318-323.doi: 10.3736/jcim20120311

• Original Clinical Research • Previous Articles     Next Articles

Effects of the Chinese patent medicine, Honghua Injection, on platelet glycoprotein Ⅱb/Ⅲa receptors in patients with acute coronary syndrome: a randomized controlled trial

Zhu Yan-feng,Luo Hai-ming(),Deng Zhong-long,Fu De-yu,Yao Wang,Dai Jian   

  1. Department of Vasculocardiology, Yueyang Hospital of Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2011-07-24 Accepted:2011-12-10 Online:2012-03-20 Published:2018-10-10

BACKGROUND: Glycoprotein (GP) Ⅱb/Ⅲa is an important index for assessing the function of platelets. To investigate the effects of Honghua Injection, a Chinese patent medicine made from extracts of Carthamus tinctorius L, on GP Ⅱb/Ⅲa is a key study in evaluating the inhibition properties of Honghua Injection on platelet aggregation.
OBJECTIVE: To observe the effects of Honghua Injection on platelet GPⅡb/Ⅲa receptors and explore the mechanisms of Honghua Injection in inhibiting platelet aggregation in patients with acute coronary syndrome.
DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: A total of 64 patients from Yueyang Hospital of Traditional Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine were randomly divided into Honghua Injection group (n=32, routine Western medicine plus Honghua Injection) and control group (n=32, routine Western medicine) according to the random number table. The patients were treated for 14 d.
MAIN OUTCOME MEASURES: The expressions of GPⅡb/Ⅲa (CD41), P-selection (CD62p), and lysosome-associated membrane GP (CD63) were measured by flow cytometry before and after treatment. Meanwhile the therapeutic effects of Huonghua Injection on angina and short-term prognosis were observed.
RESULTS: The observational period was 14 d and four patients in the control group were omitted due to early discharge. The expression of CD41 in both the Honghua Injection group and the control group was inhibited after treatment (P<0.05, P<0.01). The inhibition of the Honghua Injection group was stronger than that of the control group (P<0.05). There was no case of death, myocardial infarction and cerebral apoplexy during 14 d of treatment. The improvement of angina symptoms and electrocardiographic manifestation in the Honghua Injection group was greater than that of the control group (P<0.05).
CONCLUSION: Honghua Injection can inhibit the expression of GPⅡb/Ⅲa receptors by preventing aggregation of platelets and may be considered as an effective traditional Chinese medicine for acute coronary syndrome.

Key words: coronary disease, acute coronary syndrome, Chinese patent drugs, platelet membrane glycoprotein, platelet glycoprotein GPⅡb-Ⅲa complex;, P-selectin, randomized controlled trial

Table 1

General information of patients in two groups"

Item Control group (n=28) Treatment group (n=32)
Age (x±s, years) 72.07±10.27 69.56±11.46
Gender (male/female) 22/6 23/9
Acute myocardial infarction 14 13
Elective PCI 12 14
CABG 2 1

Figure 1

Flow diagram of this randomized trial"

Table 2

Changes of CD41, CD62p and CD63 expressions in two groups before and after treatment (x±s)"

Group n CD41 (%) Fluorescence value of CD41 CD62p (%) CD63 (%)
Control
Before treatment 28 96.12±4.10 12.01±2.69 11.16±4.27 10.82±3.74
After treatment 28 93.41±4.19* 10.36±2.63* 10.89±4.75 10.09±3.56
Treatment
Before treatment 32 96.03±3.17 12.54±3.12 10.59±4.96 11.00±4.45
After treatment 32 90.85±4.53**△ 8.85±2.91**△ 10.55±4.70 10.26±4.30

Table 3

Changes of clinical symptoms in two groups (n, %)"

Group n Obvious effective Improve Not effective Aggravated
Control 28 14 (50.0) 10 (35.7) 4 (14.3) 0 (0)
Treatment 32 23 (71.9) 7 (21.9) 2 (6.2) 0 (0)

Table 4

Changes of electrocardiogram results in two groups (n, %)"

Group n Obvious effective Improve Not effective Aggravated
Control 28 12 (42.9) 9 (32.1) 7 (25) 0 (0)
Treatment 32 21 (65.6) 8 (25.0) 3 (9.4) 0 (0)
[1] Xu DM, Jin Y, Xu WP . Carthamus tinctorius injection in treating stable angina pectoris. Zhongguo Xin Yao Yu Lin Chuang Za Zhi. 2003; 22(1): 13-15. Chinese with abstract in English.
徐东明, 金晔, 徐伟平 . 红花注射液治疗稳定型心绞痛.中国新药与临床杂志. 2003; 22(1):13-15.
[2] Li JJ, Jiang LJ, Hou YL, Liang HF , Zhao ZG. Characteristics of erythrocyte rheology in patients with coronary atherosclerotic heart disease after Honghua injection. Zhongguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 2007 ; 11 (20): 3912-3915. Chinese with abstract in English.
李俊杰, 姜丽杰, 侯亚利, 梁海峰, 赵自刚 . 冠状动脉粥样硬化性心脏病患者红细胞流变性特征在红花注射液干预后的表现.中国组织工程研究与临床康复. 2007; 11(20) : 3912-3915.
[3] Xu KL, Fan HC, Shen WY, Huang TQ , Liu LM. Clinical treatment of cerebral infarction by Safflower Injection via carotid artery infusion. Zhong Cheng Yao. 2005 ; 27(6) : 678-681. Chinese with abstract in English.
徐开蕾, 范华昌, 沈文英, 黄太权, 刘龙民 . 颈动脉灌注红花注射液治疗脑梗死的临床研究.中成药. 2005; 27(6):678-681.
[4] Guo ML, Zhang G, Zhang W, Zhang HM, Su ZW . Determination of safflor yellow A by RP-HPLC and resources quality comparison in Carthamus tinctorius. Zhongguo Zhong Yao Za Zhi. 2006; 31(15): 1234- 1236. Chinese with abstract in English.
郭美丽, 张戈, 章伟, 张汉明, 苏中武 . 红花药材黄色素A含量的RP-HPLC测定及其资源的质量评价.中国中药杂志. 2006; 31(15):1234-1236.
[5] Guo ZQ, Cheng Z, Li L, Liu QY, Wu HB, Gu Y . Effect of administration of Safflower yellow injection on the platelet aggregate rate and transforming growth factor-β1 in patients without ST elevation acute myocardial infarction. Lin Chuang Xin Xue Guan Bing Za Zhi. 2010; 26 (8): 591-593. Chinese with abstract in English.
郭张强, 成忠, 李论, 刘启云, 吴红波, 顾晔 . 红花黄色素注射液对急性非ST段抬髙型心肌梗死患者血小板聚集率和转化生长因子β1的影响.临床心血管病杂志. 2010; 26(8):591-593.
[6] Xia YY, Min Y, Sheng YC . The effects of hydroxysafflor yellow A on the formation of thrombus and platelet aggregation in rats. Zhongguo Yao Li Xue Tong Bao. 2005; 21(11): 1400-1401. Chinese with abstract in English.
夏玉叶, 闵旸, 盛雨辰 . 羟基红花黄色素A对大鼠血栓形成和血小板聚集功能的影响.中国药理学通报. 2005; 21(11) : 1400-1401.
[7] Rossi F, Rossi E, Pareti FI, Colli S, Tremoli E, Gallo L . In vitro measurement of platelet glycoprotein Eb/IIIa receptor blockade by abciximab: interindividual variation and increased platelet secretion. Haematologica. 2001 ; 86(2):192-198.
[8] Wang JY. Internal medicine. Beijing: People’s Medical Publishing House. 2005: 276-278. Chinese.
王吉耀.内科学.北京:人民卫生出版社. 2005: 276-278.
[9] Evaluation criteria for the curative effects of angina pectoris due to coronary heart disease and electrocardiographic manifestation ( Passed by the Symposium on Treatment of Angina Pectoris of Coronary Heart Disease and Arrhythmia, 1979, Shanghai). Zhongguo Yao Shi. 1987; 1(1): 71. Chinese.
冠心病心绞痛及心电图疗效评定标准( 中西医结合治疗冠心病心绞痛及心律失常座谈会, 1979, 上海). 中国药事. 1987; 1(1):71.
[10] Xu JF, Chen GF, Li ZL, Chen H . Antiplatelet and anticoagulant therapy for acute coronary syndrome. Yi Xue Zong Shu. 2010; 16(16): 2479-2482. Chinese with abstract in English.
徐杰丰, 陈国锋, 李子龙, 陈晖 . 急性冠状动脉综合征的抗血小板与抗凝治疗.医学综述. 2010; 16(16):2479-2482.
[11] Li XF, Jiang YR, Gao ZY, Yin HJ, Chen KJ . Screening, identification and analysis of platelet differential functional proteins in patients with coronary heart disease of blood-stasis pattern. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 ; 30(5): 467-473. Chinese with abstract in English.
李雪峰, 蒋跃绒, 髙铸烨, 殷慧军, 陈可冀 . 冠心病血瘀证血小板差异功能蛋白筛选、鉴定及功能分析.中国中西医结合杂志. 2010; 30(5):467-473.
[12] EPISTENT Investigators . Randomised placebo-controlled and balloon-angioplasty-controlled trial to assess safety of coronary stenting with use of platelet glycoprotein- H b/IE a blockade. Lancet. 1998; 52(9122):87-92.
[13] Maia F, Sá FC, Feres F . Glycoprotein Eb/IIIa inhibitors in clinical practice. Arq Bras Cardiol. 2009; 92(1):68-76.
doi: 10.1590/S0066-782X2009000100011
[1] Sherman Gu, Arthur Yin Fan. Controversial conclusions from two randomized controlled trials for acupuncture's effects on polycystic ovary syndrome or in vitro fertilization support. Journal of Integrative Medicine, 2020, 18(2): 89-91.
[2] Gerli E.G. Herr, Fábio Goulart da Silva, Francisco José Cidral-Filho, Fabricia Petronilho, Lucinéia Gainski Danielski, Mariana Pereira de Souza Goldim, Afonso Shiguemi Inoue Salgado, Franciane Bobinski, Daniel Fernandes Martins, Eliane R. Winkelmann. Effects of the use of bioceramic wraps in patients with lower limb venous ulcers: A randomized double-blind placebo-controlled trial. Journal of Integrative Medicine, 2020, 18(1): 27-.
[3] Xin-yu Yue, Zhuo-qi Feng, Xi-yi Yu, Jia-min Hu, Xiao-Jing He, Shi Shu. Fire-needle acupuncture for upper limb spastic paralysis after stroke: Study protocol for a randomized controlled trial. Journal of Integrative Medicine, 2019, 17(3): 167-172.
[4] Ya-ru Huangfu, Wei Peng, Bao-jun Guo, Zhi-fu Shen, Li Li, Shi-wei, Liu, Hui Zheng, You-ping Hu. Effects of acupuncture in treating insomnia due to spleen-stomach disharmony syndrome and its influence on intestinal microbiome: Study protocol for a randomized controlled trial. Journal of Integrative Medicine, 2019, 17(3): 161-167.
[5] Pádraic J.Dunne, Julie Lynch, Lucia Prihodova, Caoimhe O'Leary, Atiyeh Ghoreyshi, Sharee A. Basdeo, Donal J.Cox, Rachel Breen, Ali Sheikhi, Áine Carroll, Cathal Walsh, Geraldine McMahon, Barry White. Burnout in the emergency department: Randomized controlled trial of an attention-based training program. Journal of Integrative Medicine, 2019, 17(3): 173-180.
[6] J.M. Oliver-Baxter, H.S. Whitford, D.A. Turnbull, M.J. Bond. Effects of vitamin supplementation on inflammatory markers and psychological wellbeing among distressed women: A randomized controlled trial. Journal of Integrative Medicine, 2018, 16(5): 322-328.
[7] Parunkul Tungsukruthai, Preecha Nootim, Wiwan Worakunphanich, Nareerat Tabtong. Efficacy and safety of herbal steam bath in allergic rhinitis: A randomized controlled trial. Journal of Integrative Medicine, 2018, 16(1): 39-44.
[8] Zahra Hajmohammadi, Mojtaba Heydari, Majid Nimrouzi, Pouya Faridi, Mohammad Javad Zibaeenezhad, Gholamhossein Ranjbar Omrani, Mesbah Shams . Rhus coriaria L. increases serum apolipoprotein-A1 and high-density lipoprotein cholesterol levels: A double-blind placebo-controlled randomized clinical trial. Journal of Integrative Medicine, 2018, 16(1): 45-50.
[9] Laura Belland, Laura Rivera-Reyes, Ula Hwang. Using music to reduce anxiety among older adults in the emergency department: A randomized pilot study. Journal of Integrative Medicine, 2017, 15(6): 450-455.
[10] Hilal Yuksel, Yasemin Cayir, Zahide Kosan, Kenan Tastan. Effectiveness of breathing exercises during the second stage of labor on labor pain and duration: A randomized controlled trial. Journal of Integrative Medicine, 2017, 15(6): 456-461.
[11] Yoshiharu Motoo, Takashi Hakamatsuka, Nobuo Kawahara, Ichiro Arai, Kiichiro Tsutani. Standards of Reporting Kampo Products (STORK) in research articles. Journal of Integrative Medicine, 2017, 15(3): 182-185.
[12] Maria T.Chao, AlexandraChang, SanjayReddy, James D.Harrison, JosephAcquah, MiriaToveg, TrilceSantana, Frederick M.Hecht. Adjunctive acupuncture for pain and symptom management in the inpatient setting: Protocol for a pilot hybrid effectiveness-implementation study. Journal of Integrative Medicine, 2016, 14(3): 228-238.
[13] Nouran A. Aleyeidi, Khaled S. Aseri, Shadia M. Matbouli, Albaraa A. Sulaiamani, Sumayyah A. Kobeisy. Effects of wet-cupping on blood pressure in hypertensive patients: A randomized controlled trial. Journal of Integrative Medicine, 2015, 13(6): 391-399.
[14] Maria Sousa Cláudia, Coimbra Daniela, Machado Jorge, J. Greten Henry. Effects of self-administered exercises based on Tuina techniques on musculoskeletal disorders of professional orchestra musicians: A randomized controlled trial. Journal of Integrative Medicine, 2015, 13(5): 314-318.
[15] Marco Gemma, Elisa Nicelli, Luigi Gioia, Elena Moizo, Luigi Beretta, Maria Rosa Calvi. Acupuncture accelerates recovery after general anesthesia: A prospective randomized controlled trial. Journal of Integrative Medicine, 2015, 13(2): 99-104.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[2] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[3] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[4] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246
[5] Mei-na Ye, Hong-feng Chen, Yi-qin Cheng, Yan-sheng Zhang, Ping Li, Gang Gui, Li-ying Chen, Hao Chen, Hong-yu Dai. Study protocol for a self-controlled case study to evaluate the safety and standardization for external application of Chinese medicine Jiuyi Powder. Journal of Chinese Integrative Medicine, 2011, 9(11): 1199-1205
[6] Xiang-yu Kong, Yuan Hao, Tai-xiang Wu, Yan-ming Xie. Adverse drug reactions or adverse events of Chaihu Injection: A systematic review. Journal of Chinese Integrative Medicine, 2010, 8(12): 1124-1132
[7] Yong Miao Edwin. Clinical critical qualitative evaluation of the selected randomized controlled trials in current acupuncture researches for low back pain. Journal of Chinese Integrative Medicine, 2010, 8(12): 1133-1146
[8] Yu-tong Fei, Jian-ping Liu, He Yu, Xia Wan. Introduction to the consolidated criteria for reporting qualitative research. Journal of Chinese Integrative Medicine, 2008, 6(2): 115-118
[9] Zhu-yi Zhang, Shi-qing Yu, Li-yun Miao, Xiao-ying Huang, Xiao-ping Zhang, Yu-ping Zhu, Xiao-hong Xia, Dan-qi Li. Artesunate combined with vinorelbine plus cisplatin in treatment of advanced non-small cell lung cancer: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(2): 134-138
[10] Hui Li, Xiao-qing Liu, Bing Zhang, Li Ni, Zhi-jian Ling. Effects of active component in Cichorii on lipid metabolism of rat with hypertriglyceridemia complicated by hyperuricemia and hyperglycemia. Journal of Chinese Integrative Medicine, 2008, 6(2): 157-162