Search JIM Advanced Search

Journal of Integrative Medicine ›› 2019, Vol. 17 ›› Issue (6): 430-437.doi: 10.1016/j.joim.2019.08.001

Previous Articles     Next Articles

Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones

Rabie Kachkoula,b, Tarik Squalli Housseinic,d, Mohamed Mohimb,c, Radouane El Habbania,Youssef Miyaha, AnissaLahrichia
  

  1. a. Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco
    b. Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, BP 2202, Fez, Morocco
    c. Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco
    d. Department of Nephrology, University Hospital Hassan II, BP 1835, Atlas, Fez, Morocco
  • Online:2019-11-10 Published:2019-08-28

Abstract

Objective

The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L. leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate stones.

Methods

This survey was carried out using hydroalcoholic extract (E.FA) and infusion (I.FA) of A. unedo leaves. The quantification of phenolic compounds, flavonoids, flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultra-performance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry. Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and by the ferric reducing/antioxidant power (FRAP) assay. Litholytic activity of E.FA and I.FA was studied using a special model that resembles circuitry of the urinary system.

Results

E.FA showed greater antioxidant efficacy than I.FA (P < 0.05). Its higher efficiency was shown via the values of median inhibitory concentration, which was close to (76.14 ± 0.91) μg/mL for E.FA versus (202.64 ± 5.77) μg/mL for I.FA using the DPPH method, and (53.77 ± 0.81) μg/mL for E.FA versus (236.86 ± 31.90) μg/mL for I.FA, using FRAP method. I.FA exhibited significantly higher litholytic activity compared to E.FA (P < 0.05), with dissolution values of 31.03% ± 0.63% versus 14.55% ± 0.65%, respectively.

Conclusion

Overall, the results suggest that the A. unedo is rich in bioactive compounds, and possesses antioxidant and litholitic abilities that are worthy of further study.

Key words: Antioxidant activity, Arbutus unedo L., Litholytic activity, Phenolic compounds, Liquid chromatography, Photodiode array, Electrospray ionization tandem, Mass spectrometry

[1] Asmae Assaidi, Ikram Dib, Monique Tits, Luc Angenot, Said Bellahcen, Nourelhouda Bouanani, Abdelkhaleq Legssyer, Mohammed Aziz, Hassane Mekhfi, Mohammed Bnouham, Michel Frederich, Abderrahim Ziyyat. Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta. Journal of Integrative Medicine, 2019, 17(2): 115-124.
[2] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[3] Sandeep Kumar Singh, Arjun Patra. Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.). Journal of Integrative Medicine, 2018, 16(4): 273-282.
[4] Omar Farid, Farid Khallouki, Morad Akdad, Andrea Breuer, Robert Wyn Owen, Mohamed Eddouks. Phytochemical characterization of polyphenolic compounds with HPLC–DAD–ESI–MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides (Coss & Moq.) in normal and streptozotocin-induced diabetic rats. Journal of Integrative Medicine, 2018, 16(3): 185-191.
[5] Nanaware Sadhana, Sathiyanarayanan Lohidasan, Kakasaheb Ramoo Mahadik. Marker-based standardization and investigation of nutraceutical potential of Indian propolis. Journal of Integrative Medicine, 2017, 15(6): 483-494.
[6] Fu-feng Li, Jie Zhao, Peng Qian, Yi-qin Wang, Jing-jing Fu, Zhu-mei Sun, Hai-xia Yan, Li Yang. Metabolite changes in the greasy tongue coating of patients with chronic gastritis. Journal of Chinese Integrative Medicine, 2012, 10(7): 757-765.
[7] Hui-qing Zhang , Zi-fei Yin , Jia-yu Sheng , Zhu-qian Jiang , Bao-yu Wu , Yong-hua Su. A comparison study of pharmacokinetics between bufalin-loaded bovine serum albumin nanoparticles and bufalin in rats. Journal of Chinese Integrative Medicine, 2012, 10(6): 674-680.
[8] Venkatapura C. Devaraj, Burdipad G. Krishna, Gollapalle L. Viswanatha. Simultaneous determination of quercetin, rutin and kaempferol in the leaf extracts of Moringa oleifera Lam. and Raphinus sativus Linn. by liquid chromatography-tandem mass spectrometry. Journal of Chinese Integrative Medicine, 2011, 9(9): 1022-1030.
[9] Guo-di Lu, Jing Zhou, Hong-yue Ma, De-kang Wu, Da-wei Qian, Yu-ping Tang, Ming Hong, Yong-qing Hua, Jing-ao Duan. Analysis on competitive interaction between arenobufagin and verapamil hydrochloride with serum albumin. Journal of Chinese Integrative Medicine, 2011, 9(7): 768-774.
[10] Yong Wang, Shu-zhen Guo, Chun Li, Jian-xin Chen, Wen-jing Chuo, Fang Dong, Wei Wang, Ying-feng Wang. Analysis of plasma metabonomics of mini-swines with qi deficiency and blood stasis syndrome due to chronic myocardial ischemia. Journal of Chinese Integrative Medicine, 2011, 9(2): 158-164.
[11] Yun Ling , Hong-yu Zhao, Xin-liang Pan, Chun-xiang Zhou. Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography. Journal of Chinese Integrative Medicine, 2009, 7(11): 1057-1060.
[12] Hui Li, Ping Ren, Xi Huang, Wen-fu Tang, Hong-qiang Wei. Simultaneous determination and pharmacokinetic studies of ferulic acid and paeoniflorin in human serum by high performance liquid chromatography after oral administration of Modified Xiao-yao Decoction. Journal of Chinese Integrative Medicine, 2008, 6(11): 1178-1183.
[13] Zhi-min Liu, Xin Niu, Xue-zhi Yang, Shu-dong Niu.
Percutaneous absorption of aconitine and mesaconitine in extracts of Radix Aconitum kusnezoffii
. Journal of Chinese Integrative Medicine, 2006, 4(1): 68-72.
[14] Jun-yi Jiang, Xiang Li, Rong-li Qiu, Hai-xia Zhang, Zhi-ping Wu. Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography. Journal of Chinese Integrative Medicine, 2005, 3(1): 54-56.
[15] Chun-yan Guo, Xue-mei Bai, Wan-ming Zhang, Zhi-bao Wang, Yu-rong Fu. Determination of catalpol in dried rehmannia root and Taohong Siwu Decoction with high performance liquid chromatography. Journal of Chinese Integrative Medicine, 2004, 2(2): 135-137.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[5] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[6] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[7] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[8] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Ming-qing Wei , Jin-zhou Tian , Jing Shi , Fu-yun Ma , Ying-chun Miao , Yong-yan Wang. Effects of Chinese medicine for promoting blood circulation and removing blood stasis in treating patients with mild to moderate vascular dementia: a randomized, double-blind and parallel-controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1240-1246