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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (5): 490-494.doi: 10.3736/jcim20060510

• Original Experimental Research • Previous Articles     Next Articles

Effects of Runing Recipe medicated serum on expressions of genes in breast cancer cells

Sheng Liu1,Xiao-hong Xue1,Xin-wei Yang1,De-ming Lu2   

  1. 1. Department of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2006-09-30 Published:2006-09-15

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Objective

To explore the mechanisms of the effects of Runing Recipe in anti-invasion and anti-recurrence of breast cancer by experimental research in vitro.

Methods

SD female rats were randomly divided into Runing Recipe-treated group and its decomposed formulas Kidney-Warming Recipe and Liver-Soothing Recipe-treated groups, tamoxifen (TAM) -treated group, cyclophosphamide (CTX) -treated group, and normal control group to make medicated serums. Methods of matrigel basement membrane and real-time reverse transcription polymerase chain reaction were employed to investigate the gene expressions of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) after MDA-MB-435 cells were treated with the medicated serums.

Results

The gene expression of VEGF was dropped in CTX-treated, TAM-treated and Liver-Smoothing Recipe-treated groups. The gene expression of TIMP-1 was up-regulated in CTX-treated, Runing Recipe-treated and Kidney-Warming Recipe-treated groups; while MMP-9 was down-regulated in these groups.

Conclusion

The mechanisms of Runing Recipe in inhibiting the cancer cell invasion may be related to down-regulating the gene expressions of VEGF and MMP-9, and up-regulating the gene expression of TIMP-1.

Key words: Runing Recipe, Breast cancer, Experiment in vitro, RT-PCR

CLC Number: 

  • R285.5

Table 1

Effects of different drug-containing-serums on invasive ability of MDA-MB-435 cells in vitro (ヌ±S )"

Group n Cells number Inhibition rate (%)
Normal control 4 105.25±10.05
Runing Recipe-treated 4 67.00±9.97** 36.3
Kidney-Warming Recipe-treated 4 86.00±4.69* 18.3
Liver-Soothing Recipe-treated 4 101.25±12.71 3.8
Tamoxifen-treated 4 95.50±9.40 9.3
Cyclophosphamide-treated 4 72.25±11.09** 31.4

Table 2

Values of cycle threshold of VEGF、MMP-9 and TIMP-1 expression in 6 groups"

Group VEGF MMP-9 TIMP-1
Normal control 19.95 25.66 29.26
Runing Recipe-treated 21.25 29.09 25.36
Kidney-Warming Recipe-treated 20.37 32.73 24.56
Liver-Soothing Recipe-treated 23.50 25.63 28.70
Tamoxifen-treated 23.08 26.10 28.82
Cyclophosphamide-treated 23.43 31.83 23.93
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