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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 298-303.doi: 10.3736/jcim20080316

• Original Experimental Research • Previous Articles     Next Articles

Experimental study of Gukangling Decoction combined with technetium [99Tc] methylene diphosphonate injection in treating osteoporotic rabbits

GD Zhao1(),KJ Gao2,YM Tian1,ZW Ye2,W Li2,HP Song1,ZY Cai1   

  1. 1. Department of Traditional Chinese Internal Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
    2. Department of Nuclear Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
  • Received:2007-03-13 Online:2008-03-20 Published:2018-10-11
  • Contact: GD Zhao E-mail:chenghualong@126.com

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Objective: To evaluate the advantage of Gukangling Decoction (GKLD), a compound traditional Chinese herbal medicine, combined with technetium [ 99Tc] methylene diphosphonate injection ( 99Tc-MDP) in treating osteoporosis in rabbits.Methods: A rabbit model of osteoporosis was established by intramuscular injection of dexamethasone (DX). Fifty-six rabbits were divided into 8 groups: Group A, B, C, D, E, F, G and H. Rabbits in groups A and B were intramuscularly injected normal saline as normal control, groups C and D were untreated groups, rabbits in group E were treated by 99Tc-MDP, rabbits in group F were treated by aminodiphosphate, rabbits in group G were treated by GKLD, and rabbits in group H were treated by 99Tc-MDP and GKLD. Rabbits in groups A and C were executed to demonstrate the establishment of the rabbit model of osteoporosis at the 8th week of experiment. Rabbits in the other six groups were executed after 16-week experiment (8-week treatment), and then bone structure and cell shape were observed by electron microscope, X-ray, CT and emission computed tomography (ECT). Bone density, biomechanical parameters, the levels of bone specific alkaline phosphatase (BALP) and bone Gla protein (BGP) were measured too.Results: After 8-week of intramuscular injection of DX, the bone trabecula in group A were regular and showed normal configuration, while the bone trabecula in group C were sparse, ruptured and showed damaged form. The bone density and biomechanical parameters in group A were higher than those in group C, indicating that the rabbit model of osteoporosis was established successfully. At the 9th week of experiment, the results of cell pathology in group D showed that the bone trabeculas were sparse, ruptured, defected or had hollow section, but the bone trabeculas in group B were regular and dense. The bone trabeculas in groups H and E were restored, and were thicker than those in group D. The bone quality in groups H and E was better than group D significantly, the bone quality in group F was better than group G, and the bone quality in group G was better than group D slightly.Conclusion: GKLD combined with 99Tc-MDP had superiority in treating osteoporosis of rabbits as compared with the respective single therapy.

Key words: osteoporosis, technetium Tc 99m medronate, therapy, rabbits

CLC Number: 

  • R681.4

Table 1

Experimental parameters of groups A and C ($\bar{x}$±s)"

Group n Bone density (g/cm3) Bone biomechanics (N)
Left femoral headL4 Left femoral head L4
A 7 0.29±0.02 0.30±0.02 405.34±55.41 750.87±53.72 131.50±21.85 7.54±0.42 12.49±1.51 115.30±16.79
C 7 0.24±0.02** 0.24±0.02 265.91±52.77** 369.67±94.92 66.23±10.85** 8.23±0.71** 45.00±7.30** 168.08±17.43**

Table 2

Experimental parameters after 16-week treatment ($\bar{x}$±s)"

Group n Bone density (g/cm3) Bone biomechanics (N) Bone trabecula volume (%) Radionuclide bone imaging Blood BALP (U/L) Blood BGP (mg/L)
Left femoral head L4 Left femoral head L4
B 6 0.31±0.01 0.30±0.02 404.43±43.66 698.38±77.52 121.60±33.45 4.13±0.82 9.00±2.83 104.46±14.69
D 5 0.24±0.01 0.23±0.02 269.43±40.60 349.35±57.29 65.28±21.91 6.73±0.84 42.83±14.55 176.18±26.34
E 7 0.29±0.04* 0.30±0.05* 386.46±77.08* 649.50±126.86* 132.31±48.79** 6.03±0.73** 12.71±4.35** 135.23±13.19**
F 7 0.26±0.03* 0.27±0.04* 364.43±66.51* 451.46±121.71* 108.96±43.21** 6.24±0.72** 19.57±6.85** 149.31±20.21**
G 6 0.24±0.02 0.24±0.05 282.27±45.04 347.35±63.42 70.31±25.48 6.52±0.85 39.83±5.02 151.58±19.78
H 7 0.30±0.04*△▲ 0.31±0.03*△▲ 432.77±17.53* 615.44±76.56* 129.41±14.63** 6.15±0.60** 10.86±1.87** 123.13±17.59**

Figure 1

Cytology characteristics of bone tissue in different groups A: Group B; B: Group D; C: Group E; D: Group F; E: Group G; F: Group H."

[1] Sun D. Radioactive nuclide bone imaging(1st ed)[M]. Hangzhou: Zhejiang University Press, 2000,( 1st ed):238-240
孙达 . 放射性核素骨显像(第1版)[M]. 杭州: 浙江大学出版社, 2000,( 第1版):238-240
[2] Pan HL, He D . Changes of biochemical indexes of bone metabolism for evaluation of the characteristics in patients with senile osteoporosis[J]. Zhongguo Lin Chuang Kang Fu, 2004,8(18):3596-3597
doi: 10.3321/j.issn:1673-8225.2004.18.078
潘宏良, 何德 . 老年性骨质疏松症患者骨代谢生化指标变化对其特征的评估应用[J]. 中国临床康复, 2004,8(18):3596-3597
doi: 10.3321/j.issn:1673-8225.2004.18.078
[3] Gao KJ, Ye ZW, Li W , et al. The study of 99Tc-MDP and BONING in the treatment on the animal osteoporosis model[J]. Zhonghua Yi Xue Shi Jian Za Zhi, 2005,4(9):865-867
高克加, 叶智卫, 李伟 , 等. 99Tc-MDP与“博宁”治疗骨质疏松动物模型分析[J]. 中华医学实践杂志, 2005,4(9):865-867
[4] Zhao WX, Li JH . Pharmacotherapy progresses of osteoporosis[J]. Yi Xue Yan Jiu Sheng Xue Bao, 2003,16(5):386-388
赵文新, 李金恒 . 骨质疏松症的药物治疗进展[J]. 医学研究生学报, 2003,16(5):386-388
[5] Sun J, Zhuge TY, Wang XX . A clinical study on the treatment of primary osteoporosis(POP) by "Migu" Capsule[J]. Zhongguo Zhong Yi Gu Shang Ke Za Zhi, 2003,11(6):37-40
孙捷, 诸葛天瑜, 王西迅 , 等. 密骨胶囊治疗老年性骨质疏松症疗效观察[J]. 中国中医骨伤科杂志, 2003,11(6):37-40
[6] Dong YL . Clinical observation of Liuwei Dihuang Pill in treatment of 60 cases of osteoporosis with deficiency syndrome[J]. Zhong Yi Yao Lin Chuang Za Zhi, 2004,16(2):135-136
doi: 10.3969/j.issn.1008-7486.2004.02.016
唐凤元, 石展英 . 中西医结合治疗骨质疏松性胸腰椎骨折疗效观察[J]. 广西中医学院学报, 2004,16(2):135-136
doi: 10.3969/j.issn.1008-7486.2004.02.016
[7] Tang FY, Shi ZY . Clinical observation of integrated traditional Chinese and Western medicine therapy on osteoporotic fracture of thoracic and lumbar vertebra[J]. Guangxi Zhong Yi Xue Yuan Xue Bao, 2004,7(2):31-32
doi: 10.3969/j.issn.1008-7486.2004.02.016
唐凤元, 石展英 . 中西医结合治疗骨质疏松性胸腰椎骨折疗效观察[J]. 广西中医学院学报, 2004,7(2):31-32
doi: 10.3969/j.issn.1008-7486.2004.02.016
[8] Ma JX, Liu XJ. Practical clinical nuclear medicine(1st ed)[M]. Beijing: Atomic Energy Press, 2002,( 1st ed):516-521
马寄晓, 刘秀杰 . 实用临床核医学(第1版)[M]. 北京: 原子能出版社实用临床核医学, 2002,( 第1版):516-521
[9] Song XW, Shi YY, Shen PZ . Effect of Chinese kidney-tonifying drug on the bone of osteoporotic rat[J]. Zhongguo Gu Shang, 1997,10(5):13-14
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
宋献文, 石印玉, 沈培芝 . 补肾中药对骨质疏松大鼠骨骼的影响[J]. 中国骨伤, 1997,10(5):13-14
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
[10] Song XW, Song ZJ, Shen PZ . Clinical observation on Chinese herbs for invigorating kidney on bone mineral density in women with postmenopausal osteoporosis[J]. Shi Yong Zhong Xi Yi Jie He Za Zhi, 1997,10(9):892
宋献文, 宋中建, 沈培芝 . 补肾中药治疗绝经后骨质疏松症的临床观察[J]. 实用中西医结合杂志, 1997,10(9):892
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