SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (3): 298-303.doi: 10.3736/jcim20080316

• Original Experimental Research • Previous Articles     Next Articles

Experimental study of Gukangling Decoction combined with technetium [99Tc] methylene diphosphonate injection in treating osteoporotic rabbits

GD Zhao1(),KJ Gao2,YM Tian1,ZW Ye2,W Li2,HP Song1,ZY Cai1   

  1. 1. Department of Traditional Chinese Internal Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
    2. Department of Nuclear Medicine, Central Hospital of Huangpu District, Shanghai 200002, China
  • Received:2007-03-13 Online:2008-03-20 Published:2018-10-11
  • Contact: GD Zhao E-mail:chenghualong@126.com

RichHTML

PDF (PC)

74

Cited

12

Like

Objective: To evaluate the advantage of Gukangling Decoction (GKLD), a compound traditional Chinese herbal medicine, combined with technetium [ 99Tc] methylene diphosphonate injection ( 99Tc-MDP) in treating osteoporosis in rabbits.Methods: A rabbit model of osteoporosis was established by intramuscular injection of dexamethasone (DX). Fifty-six rabbits were divided into 8 groups: Group A, B, C, D, E, F, G and H. Rabbits in groups A and B were intramuscularly injected normal saline as normal control, groups C and D were untreated groups, rabbits in group E were treated by 99Tc-MDP, rabbits in group F were treated by aminodiphosphate, rabbits in group G were treated by GKLD, and rabbits in group H were treated by 99Tc-MDP and GKLD. Rabbits in groups A and C were executed to demonstrate the establishment of the rabbit model of osteoporosis at the 8th week of experiment. Rabbits in the other six groups were executed after 16-week experiment (8-week treatment), and then bone structure and cell shape were observed by electron microscope, X-ray, CT and emission computed tomography (ECT). Bone density, biomechanical parameters, the levels of bone specific alkaline phosphatase (BALP) and bone Gla protein (BGP) were measured too.Results: After 8-week of intramuscular injection of DX, the bone trabecula in group A were regular and showed normal configuration, while the bone trabecula in group C were sparse, ruptured and showed damaged form. The bone density and biomechanical parameters in group A were higher than those in group C, indicating that the rabbit model of osteoporosis was established successfully. At the 9th week of experiment, the results of cell pathology in group D showed that the bone trabeculas were sparse, ruptured, defected or had hollow section, but the bone trabeculas in group B were regular and dense. The bone trabeculas in groups H and E were restored, and were thicker than those in group D. The bone quality in groups H and E was better than group D significantly, the bone quality in group F was better than group G, and the bone quality in group G was better than group D slightly.Conclusion: GKLD combined with 99Tc-MDP had superiority in treating osteoporosis of rabbits as compared with the respective single therapy.

Key words: osteoporosis, technetium Tc 99m medronate, therapy, rabbits

CLC Number: 

  • R681.4

Table 1

Experimental parameters of groups A and C ($\bar{x}$±s)"

Group n Bone density (g/cm3) Bone biomechanics (N)
Left femoral headL4 Left femoral head L4
A 7 0.29±0.02 0.30±0.02 405.34±55.41 750.87±53.72 131.50±21.85 7.54±0.42 12.49±1.51 115.30±16.79
C 7 0.24±0.02** 0.24±0.02 265.91±52.77** 369.67±94.92 66.23±10.85** 8.23±0.71** 45.00±7.30** 168.08±17.43**

Table 2

Experimental parameters after 16-week treatment ($\bar{x}$±s)"

Group n Bone density (g/cm3) Bone biomechanics (N) Bone trabecula volume (%) Radionuclide bone imaging Blood BALP (U/L) Blood BGP (mg/L)
Left femoral head L4 Left femoral head L4
B 6 0.31±0.01 0.30±0.02 404.43±43.66 698.38±77.52 121.60±33.45 4.13±0.82 9.00±2.83 104.46±14.69
D 5 0.24±0.01 0.23±0.02 269.43±40.60 349.35±57.29 65.28±21.91 6.73±0.84 42.83±14.55 176.18±26.34
E 7 0.29±0.04* 0.30±0.05* 386.46±77.08* 649.50±126.86* 132.31±48.79** 6.03±0.73** 12.71±4.35** 135.23±13.19**
F 7 0.26±0.03* 0.27±0.04* 364.43±66.51* 451.46±121.71* 108.96±43.21** 6.24±0.72** 19.57±6.85** 149.31±20.21**
G 6 0.24±0.02 0.24±0.05 282.27±45.04 347.35±63.42 70.31±25.48 6.52±0.85 39.83±5.02 151.58±19.78
H 7 0.30±0.04*△▲ 0.31±0.03*△▲ 432.77±17.53* 615.44±76.56* 129.41±14.63** 6.15±0.60** 10.86±1.87** 123.13±17.59**

Figure 1

Cytology characteristics of bone tissue in different groups A: Group B; B: Group D; C: Group E; D: Group F; E: Group G; F: Group H."

[1] Sun D. Radioactive nuclide bone imaging(1st ed)[M]. Hangzhou: Zhejiang University Press, 2000,( 1st ed):238-240
孙达 . 放射性核素骨显像(第1版)[M]. 杭州: 浙江大学出版社, 2000,( 第1版):238-240
[2] Pan HL, He D . Changes of biochemical indexes of bone metabolism for evaluation of the characteristics in patients with senile osteoporosis[J]. Zhongguo Lin Chuang Kang Fu, 2004,8(18):3596-3597
doi: 10.3321/j.issn:1673-8225.2004.18.078
潘宏良, 何德 . 老年性骨质疏松症患者骨代谢生化指标变化对其特征的评估应用[J]. 中国临床康复, 2004,8(18):3596-3597
doi: 10.3321/j.issn:1673-8225.2004.18.078
[3] Gao KJ, Ye ZW, Li W , et al. The study of 99Tc-MDP and BONING in the treatment on the animal osteoporosis model[J]. Zhonghua Yi Xue Shi Jian Za Zhi, 2005,4(9):865-867
高克加, 叶智卫, 李伟 , 等. 99Tc-MDP与“博宁”治疗骨质疏松动物模型分析[J]. 中华医学实践杂志, 2005,4(9):865-867
[4] Zhao WX, Li JH . Pharmacotherapy progresses of osteoporosis[J]. Yi Xue Yan Jiu Sheng Xue Bao, 2003,16(5):386-388
赵文新, 李金恒 . 骨质疏松症的药物治疗进展[J]. 医学研究生学报, 2003,16(5):386-388
[5] Sun J, Zhuge TY, Wang XX . A clinical study on the treatment of primary osteoporosis(POP) by "Migu" Capsule[J]. Zhongguo Zhong Yi Gu Shang Ke Za Zhi, 2003,11(6):37-40
孙捷, 诸葛天瑜, 王西迅 , 等. 密骨胶囊治疗老年性骨质疏松症疗效观察[J]. 中国中医骨伤科杂志, 2003,11(6):37-40
[6] Dong YL . Clinical observation of Liuwei Dihuang Pill in treatment of 60 cases of osteoporosis with deficiency syndrome[J]. Zhong Yi Yao Lin Chuang Za Zhi, 2004,16(2):135-136
doi: 10.3969/j.issn.1008-7486.2004.02.016
唐凤元, 石展英 . 中西医结合治疗骨质疏松性胸腰椎骨折疗效观察[J]. 广西中医学院学报, 2004,16(2):135-136
doi: 10.3969/j.issn.1008-7486.2004.02.016
[7] Tang FY, Shi ZY . Clinical observation of integrated traditional Chinese and Western medicine therapy on osteoporotic fracture of thoracic and lumbar vertebra[J]. Guangxi Zhong Yi Xue Yuan Xue Bao, 2004,7(2):31-32
doi: 10.3969/j.issn.1008-7486.2004.02.016
唐凤元, 石展英 . 中西医结合治疗骨质疏松性胸腰椎骨折疗效观察[J]. 广西中医学院学报, 2004,7(2):31-32
doi: 10.3969/j.issn.1008-7486.2004.02.016
[8] Ma JX, Liu XJ. Practical clinical nuclear medicine(1st ed)[M]. Beijing: Atomic Energy Press, 2002,( 1st ed):516-521
马寄晓, 刘秀杰 . 实用临床核医学(第1版)[M]. 北京: 原子能出版社实用临床核医学, 2002,( 第1版):516-521
[9] Song XW, Shi YY, Shen PZ . Effect of Chinese kidney-tonifying drug on the bone of osteoporotic rat[J]. Zhongguo Gu Shang, 1997,10(5):13-14
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
宋献文, 石印玉, 沈培芝 . 补肾中药对骨质疏松大鼠骨骼的影响[J]. 中国骨伤, 1997,10(5):13-14
doi: doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz
[10] Song XW, Song ZJ, Shen PZ . Clinical observation on Chinese herbs for invigorating kidney on bone mineral density in women with postmenopausal osteoporosis[J]. Shi Yong Zhong Xi Yi Jie He Za Zhi, 1997,10(9):892
宋献文, 宋中建, 沈培芝 . 补肾中药治疗绝经后骨质疏松症的临床观察[J]. 实用中西医结合杂志, 1997,10(9):892
[1] Tara Rajendran. Addressing the need for personalizing music therapy in integrative oncology. Journal of Integrative Medicine, 2022, 20(4): 281-283.
[2] Tara Rajendran, Martha Summa-Chadwick. The scope and potential of music therapy in stroke rehabilitation. Journal of Integrative Medicine, 2022, 20(4): 284-287.
[3] Lu Yin, Yun An, Xiao Chen, Hui-xin Yan, Tao Zhang, Xin-gang Lu, Jun-tao Yan. Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury. Journal of Integrative Medicine, 2022, 20(3): 265-273.
[4] Bin Zhao, Yong Chen, Shi-min Liao, Jian-ying Zheng, Shi-hua Yan, Dong-shu Zhang. Successfully treated recalcitrant atopic eczema with acupoint autohemotherapy: A case report and hypothesized mechanism of the therapy. Journal of Integrative Medicine, 2022, 20(2): 182-186.
[5] Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[6] Joon-ho Lee, Yuan-ji Wei, Zhong-yan Zhou, Yu-ming Hou, Cheng-long Wang, Li-bo Wang, Hong-jin Wu, Yu Zhang, Wei-wei Dai. Efficacy of the herbal pair, Radix Achyranthis Bidentatae and Eucommiae Cortex, in preventing glucocorticoid-induced osteoporosis in the zebrafish model. Journal of Integrative Medicine, 2022, 20(1): 83-90.
[7] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[8] Gai Ran, Xi-lin Feng, Yi-lin Xie, Qing-yun Zheng, Peng-peng Guo, Ming Yang, Ying-lu Feng, Chen Ling, Li-qing Zhu, Chen Zhong. The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy. Journal of Integrative Medicine, 2021, 19(6): 515-525.
[9] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[10] Zahra Karimzadeh, Mansooreh Azizzadeh Forouzi, Haleh Tajadini, Mehdi Ahmadinejad, Callista Roy, Mahlagha Dehghan. Effects of lavender and Citrus aurantium on pain of conscious intensive care unit patients: A parallel randomized placebo-controlled trial. Journal of Integrative Medicine, 2021, 19(4): 333-339.
[11] Ning Zhang, Xiao-he Xiao. Integrative medicine in the era of cancer immunotherapy: Challenges and opportunities. Journal of Integrative Medicine, 2021, 19(4): 291-294.
[12] Riska Siregar, Aris Setyawan, Syahruramdhani Syahruramdhani. A model to standardize safety and quality of care for cupping therapy. Journal of Integrative Medicine, 2021, 19(4): 327-332.
[13] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[14] Nurul Afiedia Roslim, Aryati Ahmad, Mardiana Mansor, Myat Moe Thwe Aung, Farrahdilla Hamzah, Haszalina Hassan, Pei Lin Lua. Hypnotherapy for overweight and obese patients: A narrative review. Journal of Integrative Medicine, 2021, 19(1): 1-5.
[15] Johannes Loeser, Brigitte Layer, Christopher Plata, Klaus-Maria Perrar, Tim Hucho, Rebecca Kulbida. Hirudotherapy attenuates arthritic pain in patients with various chronic pain syndromes: A retrospective analysis. Journal of Integrative Medicine, 2020, 18(5): 425-433.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3