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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (10): 1101-1109.doi: 10.3736/jcim20111011

• Original Clinical Research • Previous Articles     Next Articles

Assessing the microcirculatory response following oral administration of Liuwei Dihuang formula by spectral analysis of skin-surface laser Doppler signals

Hsin Hsiu1(), Wei-Chen Hsu2, Shih-Ming Huang3, Chia-Ching Lin3, Hung-Wen Lin3   

  1. 1. Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, China
    2. Department of Traditional Chinese Medicine, Taipei City Hospital RenAi Branch, Taipei, Taiwan, China
    3. Department of Electrical Engineering, Yuan Ze University, Taoyuan, Taiwan, China
  • Received:2011-05-10 Accepted:2011-06-15 Online:2011-10-20 Published:2011-10-15
  • Contact: Hsiu Hsin E-mail:hhsiu@mail.ntust.edu.tw

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Objective: This study aimed to assess the skin-surface microcirculatory effects of oral administration of Liuwei Dihuang (LW) formula by analyzing the frequency content of laser-Doppler skin blood-flow signals at important acupoints.

Methods: Laser-Doppler signals were measured in male healthy volunteers in two groups of experiments: the LW experiment (n=42; aged (27.8±3.8) years) and the control experiment (n=28; aged (27.3±3.4) years). Each experiment involved a 20-minute baseline-data sequence and three following sets of effects data: recorded on the same day after oral administration of 250 mL water (28 ℃) accompanied with 5 g of LW formula (herbal extract granules), and recorded on the second day after taking other two doses of LW formula.

Results: During the second day, the increases in the mean and pulsatile components of the flux signal were the most prominent, and the relative energy contribution at 0.02 to 0.06 Hz was significantly decreased at Taixi (KI3). There were no any other significant changes in these parameters at other measurement sites.

Conclusion: The redistribution of skin-surface microcirculatory blood flow following oral LW administration observed here can be attributed to different changes in sympathetic neural activities and thus the different perfusion resistance through the arteriolar openings of the peripheral vascular beds at different measurement sites. The developed noninvasive and real-time monitoring technique could form part of an evidence-based method for studying the physiological effects and the underlying mechanisms of traditional Chinese medicine herbs.

Key words: Liuwei Dihuang decoction, drugs,Chinese herbal, microcirculation, laser-Doppler flowmetry, human body

Figure 1

Locations of the measurement sites on the footO: Taixi acupoint (Site 1); ×: nearby nonacupoint (Site 2); △: location of the thermistor."

"

Skin temperature (℃)
Group Site n M0 M1 M2 M3
Control Site 1 28 31.5±1.5 31.6±1.4 31.1±1.5 31.4±1.6
Site 3 28 31.3±1.3 31.5±1.4 31.8±1.4 31.3±1.6
Site 4 28 33.2±1.3 33.3±1.2 33.0±1.2 32.9±1.5
LW Site 1 42 31.6±1.3 31.7±1.2 31.9±1.2 31.7±1.3
Site 3 42 32.0±1.2 32.1±1.5 31.9±1.4 32.4±1.2
Site 4 42 33.2±1.1 33.2±0.8 33.3±1.1 33.0±0.9

Figure 2

Definitions of the LDF parametersTime 0 corresponds to the location of the ECG R-peak. MMBF is the mean value of the LDF flux signals during each 20-minute measurement period while PMBF is the mean value of the absolute values of all data points in the DC-filtered LDF waveform (i.e., the average value of the shaded area). LDF: laser-Doppler flowmetry; ECG: electrocardiograph."

"

Group Time n SBP (mmHg) DBP (mmHg) HR (beats/min) Environmental temperature (℃)
Control Day 1 28 114.0±6.4 77.6±5.5 70.3±6.4 23.0±0.9
Day 2 28 114.3±6.8 76.0±5.7 70.8±7.1 23.2±0.8
LW Day 1 42 111.5±6.0 75.4±5.6 70.0±6.4 23.2±0.7
Day 2 42 112.0±6.6 75.3±5.2 71.2±7.5 22.9±0.8

"

Group Period n Relative change
Site 1 Site 2 Site 3 Site 4
LW M1/M0 42 1.103±0.107* 1.083±0.115* 1.018±0.094 1.034±0.114
M2/M0 42 1.137±0.202* 1.141±0.249* 1.102±0.196* 1.048±0.220
M3/M0 42 1.211±0.384* 1.091±0.208* 1.124±0.264* 0.978±0.187
Control M1/M0 28 1.018±0.091 1.014±0.064 0.986±0.123 1.021±0.083
M2/M0 28 0.958±0.121 0.991±0.122 0.969±0.139 1.030±0.138
M3/M0 28 0.971±0.110 0.979±0.132 0.972±0.119 1.015±0.128

"

Group Period n Relative change
Site 1 Site 2 Site 3 Site 4
LW M1/M0 42 1.117±0.178 1.068±0.158 0.991±0.196 1.066±0.151
M2/M0 42 1.232±0.265* 1.180±0.266* 1.043±0.354 0.968±0.211
M3/M0 42 1.369±0.270* 1.130±0.258 1.095±0.484 0.979±0.261
Control M1/M0 18 1.044±0.125 1.010±0.118 1.027±0.145 1.041±0.138
M2/M0 18 0.979±0.138 1.017±0.147 0.987±0.149 0.978±0.170
M3/M0 18 1.031±0.155 0.989±0.144 1.041±0.151 1.030±0.148

"

Site Period Change in REC (%)
FR1 FR2 FR3 FR4 FR5
Site 1 M0 20.72±6.83 27.29±5.60 20.20±7.67 11.49±5.20 20.30±10.14
M1 20.70±7.61 25.43±6.17 19.85±7.71 13.14±6.10 20.87±11.31
M2 20.27±8.93 24.39±5.19* 20.93±7.20 12.24±5.48 22.17±12.63
M3 18.08±6.85 22.76±4.77* 22.21±8.29 12.70±8.12 24.24±10.49*
Site 2 M0 13.26±4.37 18.99±6.89 19.44±6.91 19.09±7.23 29.22±9.08
M1 13.21±4.90 18.57±6.02 19.38±6.34 19.53±7.03 29.31±9.15
M2 13.69±6.10 17.49±5.76 19.33±7.29 19.94±8.30 29.56±9.56
M3 13.90±6.94 17.72±6.75 20.18±7.08 19.96±8.46 28.24±8.86
Site 3 M0 16.29±7.75 27.67±7.88 24.31±5.76 14.70±7.89 17.02±8.88
M1 17.19±6.76 26.77±6.48 24.69±6.48 14.06±6.92 17.29±9.12
M2 17.09±6.32 27.66±7.29 24.79±6.91 13.62±6.29 16.83±7.98
M3 17.38±7.26 26.60±6.80 26.14±5.22 13.55±5.29 16.33±8.87
Site 4 M0 23.69±7.78 26.81±5.33 19.53±6.73 11.46±5.68 18.51±10.52
M1 24.48±8.77 25.36±5.90 19.02±7.05 12.66±6.05 18.48±10.97
M2 25.46±9.96 25.19±5.46 19.84±7.09 11.08±4.56 18.44±11.53
M3 23.24±7.99 23.71±6.01 20.84±7.39 12.01±7.28 20.20±11.57

Figure 3

Relation between changes in the PMBF and 1/(REC of FR2) of the LDF signal at the four measurement sites during M3Relative changes in the PMBF and 1/(REC of FR2) are defined as the M3 value divided by the average baseline value. R2 is 0.51 (P<0.01 by F-test) at Site 1, which indicates that the most prominent inverse proportional relation between PMBF and the REC of FR2. The R2 values are all smaller than 0.20 at other sites. PMBF: pulsatile microcirculatory blood flow; REC: relative energy contribution; FR: frequency; LDF: laser-Doppler flowmetry."

[1] Yang S, Zhou W, Zhang Y, Yan C, Zhao Y . Effects of Liuwei Dihuang decoction on ion channels and synaptic transmission in cultured hippocampal neuron of rat[J]. J Ethnopharmacol, 2006,106(2):166-172
doi: 10.1016/j.jep.2005.12.017
[2] Niimi H, Han JY, Patumraj S . Asian traditional medicine(ATM) based on in vivo microcirculation evidence[J]. Clin Hemorheol Microcirc, 2003,29(3-4):195-198
doi: 10.1200/JCO.2004.12.082 pmid: 14724341
[3] Hsiu H, Hsu WC, Chang SL, Hsu CL, Huang SM, Lin YY . Microcirculatory effect of different skin contacting pressures around the blood pressure[J]. Physiol Meas, 2008,29(12):1421-1434
doi: 10.1088/0967-3334/29/12/006 pmid: 18974438
[4] Hsiu H, Hsu WC, Huang SM, Hsu CL, Lin Wang YY . Spectral analysis of the microcirculatory laser Doppler signal at the Hoku acupuncture point[J]. Lasers Med Sci, 2009,24(3):353-358
doi: 10.1007/s10103-008-0569-8 pmid: 18504639
[5] Hsiu H, Hsu WC, Hsu CL, Huang SM, Lin YY . Microcirculatory changes by laser Doppler after infrared heating over acupuncture points - relevance to moxibustion[J]. Photomed Laser Surg, 2009,27(6):855-861
doi: 10.1089/pho.2008.2390
[6] Stefanovska A, Bracic M, Kvernmo HD . Wavelet analysis of oscillations in the peripheral blood circulation measured by laser Doppler technique[J]. IEEE Trans Biomed Eng, 1999,46(10):1230-1239
doi: 10.1109/10.790500
[7] Chao PT, Jan MY, Hsiu H, Hsu TL, Wang WK, Lin Wang YY . Evaluating microcirculation by pulsatile laser Doppler signal[J]. Phys Med Biol, 2006,51(4):845-854
doi: 10.1002/1438-9312(200102)103:23.0.CO;2-N pmid: 16467582
[8] Jan MY, Hsiu H, Hsu TL, Wang YY, Wang WK . The importance of pulsatile microcirculation in relation to hypertension[J]. IEEE Eng Med Biol Mag, 2000,19(3):106-111
doi: 10.1109/51.844389 pmid: 10834125
[9] Lee JJ, Tyml K, Menkis AH, Novick RJ, Mckenzie FN . Evaluation of pulsatile and nonpulsatile flow in capillaries of goat skeletal muscle using intravital microscopy[J]. Microvasc Res, 1994,48(3):316-327
doi: 10.1006/mvre.1994.1058
[10] Seki J, Satomura Y, Ooi Y . Velocity pulse advances pressure pulse by close to 45 degrees in the rat pial arterioles[J]. Biorheology, 2004,41(1):45-52
doi: 10.1002/bip.10520 pmid: 14967889
[11] Hsiu H, Chao PT, Hsu WC, Jan MY, Wang YY, Wang WK . The possible role of arterial radial vibration in heart rate and blood pressure matching[J]. Proc Inst Mech Eng H, 2008,222(5):773-779
doi: 10.1243/09544119JEIM349 pmid: 18756694
[12] Hsu TL, Chao PT, Jan MY, Wang WK, Li SP, Wang YY . Effects of angiotensin Ⅱ on flux rise time in rats(a time index of laser Doppler flowmetry) and its relation with microvascular structures[J]. Microvasc Res, 2008,75(2):211-216
doi: 10.1016/j.mvr.2007.07.006
[13] Hsiu H, Huang SM, Chao PT, Jan MY, Hsu TL, Wang WK, Wang YY . Microcirculatory characteristics of acupuncture points obtained by laser Doppler flowmetry[J]. Physiol Meas, 2007,28(10):N77-N86
doi: 10.1088/0967-3334/28/10/N01 pmid: 17906382
[14] Hsiu H, Hsu WC, Chen BH, Hsu CL . Differences in the microcirculatory effects of local skin surface contact pressure stimulation between acupoints and nonacupoints: possible relevance to acupressure[J]. Physiol Meas, 2010,31(6):829-841
doi: 10.1088/0967-3334/31/6/007
[15] Hsiu H, Hsu WC, Hsu CL , Huang SM . Assessing the effects of acupuncture by comparing needling the Hegu acupoint and needling nearby nonacupoints by spectral analysis of microcirculatory laser Doppler signals. Evid Based Complement Alternat Med. 2011; [ Epub ahead of print].
[16] Ren DC, Du GH, Zhang JT . Protective effect of Ginkgo biloba extract on endothelial cell against damage induced by oxidative stress[J]. J Cardiovasc Pharmacol, 2002,40(6):809-814
doi: 10.1097/00005344-200212000-00001
[17] Zhang J, Fu S, Liu S, Mao T, Xiu R . The therapeutic effect of Ginkgo biloba extract in SHR rats and its possible mechanisms based on cerebral microvascular flow and vasomotion[J]. Clin Hemorheol Microcirc, 2000,23(2-4):133-138
[18] Neuhofer W, Pittrow D . Role of endothelin and endothelin receptor antagonists in renal disease[J]. Eur J Clin Invest, 2006,36(Suppl 3):78-88
doi: 10.1111/j.1365-2362.2006.01689.x pmid: 16919017
[19] Tigno XT, Gumila E . In vivo microvascular actions of Artemisia vulgaris L. in a model of ischemia-reperfusion injury in the rat intestinal mesentery[J]. Clin Hemorheol Microcirc, 2000,23(2-4):159-165
[20] Hsiu H, Hsu CL, Chiang WR, Chao PT, Hsu TL, Jan MY, Wang WK, Wang YY . Connection between RR-interval length and the pulsatile microcirculatory flow[J]. Physiol Meas, 2008,29(2):245-254
doi: 10.1088/0967-3334/29/2/007 pmid: 18256455
[21] Hsiu H, Hsu CL, Jan MY . Relations between beat-to-beat microcirculatory blood flow and variations therein[J]. Photomed Laser Surg, 2010,28(6):785-792
doi: 10.1089/pho.2009.2690 pmid: 21142723
[22] Hsiu H, Hsu WC, Hsu CL, Jan MY, Wang-Lin YY . Effects of acupuncture at the Hoku acupoint on the pulsatile laser Doppler signal at the heartbeat frequency[J]. Lasers Med Sci, 2009,24(4):553-560
doi: 10.1007/s10103-008-0610-y
[23] Hsiu H, Huang SM, Chen CT, Hsu CL, Hsu WC . Acupuncture stimulation causes bilaterally different microcirculatory effects in stroke patients[J]. Microvasc Res, 2011,81(3):289-294
doi: 10.1016/j.mvr.2011.03.001
[24] Bernardi L, Rossi M, Fratino P, Finardi G, Mevio E, Orlandi C . Relationship between phasic changes in human skin blood flow and autonomic tone[J]. Microvasc Res, 1989,37(1):16-27
doi: 10.1016/0026-2862(89)90069-1 pmid: 2921947
[25] Kvandal P, Landsverk SA, Bernjak A, Stefanovska A, Kvernmo HD , Kirkebøen KA.Low-frequency oscillations of the laser Doppler perfusion signal in human skin[J]. Microvasc Res, 2006,72(3):120-127
doi: 10.1016/j.mvr.2006.05.006 pmid: 16854436
[26] He H, Yang X, Shi M, Zeng X, Yang J, Wu L, Li L . Protective effects of hydroxysafflor yellow A on acute and chronic congestive cardiac failure mediated by reducing ET-1, NOS and oxidative stress in rats[J]. J Pharm Pharmacol, 2008,60(1):115-123
[27] Liu HR, Tang XY, Dai DZ, Dai Y . Ethanol extracts of Rehmannia complex(Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats[J]. J Ethnopharmacol, 2008,118(3):466-472
doi: 10.1016/j.jep.2008.05.015
[28] Wang WK, Hsu TL, Wang YY . Liu-wei-dihuang: a study by pulse analysis[J]. Am J Chin Med, 1998,26(1):73-82
doi: 10.1142/S0192415X98000105 pmid: 9592596
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