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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 455-458.doi: 10.3736/jcim20050609

• Original Clinical Research • Previous Articles     Next Articles

Effects of Liuwei Dihuang Pills on expressions of apoptosis-related genes bcl-2 and Bax in pancreas of OLETF rats

Yao-ming Xue1,Ren Luo2,Bo Zhu1,Yan Zhang1,Yong-hua Pan1,Chen-zhong Li1   

  1. 1. Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China
    2. Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China
  • Received:2005-08-15 Online:2005-11-20 Published:2005-11-20
  • Contact: Yao-Ming XUE E-mail:brightxue@163.com

Objective

To investigate the effects of Liuwei Dihuang Pills (LWDHP) on expressions of apoptosis-related genes bcl-2 and Bax in pancreas of OLETF rats.

Methods

Forty male OLETF rats were randomly divided into LWDHP-treated group and untreated group. Another ten male LETO rats were included in normal control group. OLETF rats in the LWDHP-treated group were given LWDHP (2.4 g·kg -1·d -1) orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. Body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerance test (OGTT). Pancreas weights were recorded after rats were killed and the expression levels of bcl-2 mRNA and Bax mRNA were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results

In the LWDHP-treated group, the expression of bcl-2 mRNA in the pancreas of rats at the age of 40 weeks (1.25±0.07) was much higher than that in the untreated group (1.01±0.16), P<0.01. Bax mRNA level in the LWDHP-treated group (0.57±0.11) was obviously lower than that in the untreated group (1.18±0.28), P<0.01. There was no significant difference of pancreas-to-body weight ratios between the LWDHP-treated group and the untreated group. The ability of glucose tolerance was improved in the LWDHP-treated group.

Conclusion

LWDHP can up-regulate the expression of bcl-2 and down-regulate the expression of Bax at transcription level, which maybe contribute to the anti-apoptosis effects of LWDHP.

Key words: Diabetes mellitus, Type 2, Apoptosis, Gene bcl-2, Gene Bax, Liuwei Dihuang Decoction, Rats, OLETF

CLC Number: 

  • R587.1

Tab 1

Blood glucose concentration and AUC of rats in 3 groups ($\bar{x}$±s )"

Group n PBG 2 h
(mmol/L)
AUC
(mmol/L)
Normal control group (LETO)
at age of 30 weeks 7 7.5±0.9 28.8±1.7
at age of 34 weeks 4 8.6±1.2 30.6±1.7
at age of 40 weeks 4 7.9±1.2 29.8±0.6
Untreated group (OLETF)
at age of 30 weeks 17 10.5±1.9 46.5±4.4△△
at age of 34 weeks 14 13.7±4.2 57.6±10.2△△
at age of 40 weeks 14 14.8±3.2 63.4±8.9△△
LWDHP-treated
at age of 30 weeks 20 9.4±2.3 40.4±10.3*△△
at age of 34 weeks 14 11.6±3.1 47.4±11.1*△△
at age of 40 weeks 14 12.0±3.0** 54.0±10.9*△△

Tab 2

Pancreas-to-body weight ratio of rats in 3 groups ($\bar{x}$±s , %)"

Group n Pancreas-to-
body weight ratio
Normal control group (LETO)
at age of 8 weeks 3 0.17±0.02
at age of 32 weeks 3 0.20±0.01
at age of 40 weeks 4 0.19±0.02
Untreated group (OLETF)
at age of 8 weeks 3 0.16±0.04
at age of 32 weeks 3 0.15±0.06
at age of 40 weeks 3 0.14±0.02**
LWDHP-treated
at age of 32 weeks 5 0.19±0.02
at age of 40 weeks 7 0.16±0.01**

Tab 3

Expression levels of bcl-2 and Bax mRNAs of rats in 3 groups ($\bar{x}$±s )"

Group n bcl-2 mRNA Bax mRNA
Normal control group (LETO)
at age of 8 weeks 3 1.48 0.19 0.26 0.05
at age of 32 weeks 3 1.42±0.20 0.28±0.12**
at age of 40 weeks 4 1.33±0.11** 0.41±0.08**
Untreated group (OLETF)
at age of 8 weeks 3 1.36±0.22 0.23±0.04
at age of 32 weeks 3 1.11±0.06 1.31±0.10
at age of 40 weeks 3 1.01±0.16 1.18±0.28
LWDHP-treated
at age of 32 weeks 5 1.29±0.17 0.28±0.17**
at age of 40 weeks 7 1.25±0.07** 0.57±0.11**
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