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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (11): 1279-1285.doi: 10.3736/jcim20121112

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Danzhi Jiangtang Capsule and exercise on JNK signaling pathway in pancreatic tissues of diabetic rats

Yuan-jie Wu1(), Zhao-hui Fang2, Shu-guo Zheng3, Yuan-bo Wu4, Ai-hua Fan5   

  1. 1. Department of Basic Theory of Traditional Chinese Medicine, Anhui College of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    2. Department of Endocrinology, the First Hospital Affiliated to Anhui College of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
    3. Department of Pharmacology, Wannan Medical College, Wuhu 241002, Anhui Province, China
    4. Department of Neurology, Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001,Anhui Province, China
    5. Department of Diagnostics, Bengbu Medical College, Bengbu 233030, Anhui Province, China
  • Received:2012-04-27 Accepted:2012-05-25 Online:2012-11-20 Published:2018-11-15

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Objective: To explore the effects of exercise and Danzhi Jiangtang Capsule (DJC), a compound traditional herbal medicine, on the JNK signaling pathway in pancreatic tissues of diabetic rats and to investigate the possible mechanisms of exercise and DJC in treating diabetes.

Methods: Seventy-eight male Wistar rats were injected with low dose of streptozotocin and fed a high-fat diet to establish a diabetic model in rats. Then 60 diabetic rats were divided into diabetes group, exercise group, DJC group and exercise combined with DJC group. Another twelve rats were used as normal control. After eight months of treatment, the expression levels of phosphor-c-Jun N-terminal kinase (p-JNK), pancreatic and duodenal homeobox-1 (PDX-1), and insulin protein in pancreatic tissues from rats were detected by immunohistochemical method and Western blotting.

Results: In pancreatic tissues of diabetes group, the expression level of p-JNK protein was significantly higher than that in the normal group (P<0.01), and the expression levels of PDX-1 and insulin protein were significantly decreased (P<0.01). After administration of exercise and DJC, the expression level of p-JNK protein in pancreatic tissues of the diabetes group was decreased significantly, while the expression levels of PDX-1 and insulin protein were increased significantly (P<0.05 or P<0.01).

Conclusion: Exercise and DJC effectively protect islet β-cell function in diabetic rats, which might be due to a decreased JNK signaling pathway.

Key words: drugs,Chinese herbal, exercise, diabetes mellitus,experimental, insulin-secreting cells, JNK mitogen-activated protein kinases, rats

Figure 1

Immunohistochemical staining for detecting insulin in pancreatic islets of rats (light microscopy, ×400) A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise plus Danzhi Jiangtang Capsule group."

Figure 2

Immunohistochemical staining for detecting PDX-1 expression in pancreatic islets of rats (light microscopy, ×400) A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group. PDX-1: pancreatic and duodenal homeobox-1."

Table 1

Expressions of PDX-1 and insulin in pancreatic islets of rats (x±s)"

Group n PDX-1 Insulin
Normal 6 0.320±0.001 0.329±0.001
Untreated 6 0.143±0.010** 0.209±0.012**
DJC 6 0.216±0.025△△ 0.294±0.035△△
Exercise 6 0.242±0.022△△ 0.293±0.025△△
Exercise plus DJC 6 0.252±0.009△△ 0.316±0.014△△

Figure 3

Expression of PDX-1 in pancreatic islets of rats Data are expressed as x±s, n=4. **P<0.01, vs normal group; △P<0.05, △△P<0.01, vs untreated group. A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group.PDX-1: pancreatic and duodenal homeobox-1."

Figure 4

Expression of p-JNK in pancreatic islets of rats Data are expressed as x±s , n=4. **P<0.01, vs normal group; △P<0.05, △△P<0.01, vs untreated group. A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group. p-JNK: phospho-c-Jun N-terminal kinase."

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