SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (11): 1279-1285.doi: 10.3736/jcim20121112

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Danzhi Jiangtang Capsule and exercise on JNK signaling pathway in pancreatic tissues of diabetic rats

Yuan-jie Wu1(), Zhao-hui Fang2, Shu-guo Zheng3, Yuan-bo Wu4, Ai-hua Fan5   

  1. 1. Department of Basic Theory of Traditional Chinese Medicine, Anhui College of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
    2. Department of Endocrinology, the First Hospital Affiliated to Anhui College of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
    3. Department of Pharmacology, Wannan Medical College, Wuhu 241002, Anhui Province, China
    4. Department of Neurology, Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001,Anhui Province, China
    5. Department of Diagnostics, Bengbu Medical College, Bengbu 233030, Anhui Province, China
  • Received:2012-04-27 Accepted:2012-05-25 Online:2012-11-20 Published:2018-11-15

RichHTML

1

PDF (PC)

41

Cited

3

Like

Objective: To explore the effects of exercise and Danzhi Jiangtang Capsule (DJC), a compound traditional herbal medicine, on the JNK signaling pathway in pancreatic tissues of diabetic rats and to investigate the possible mechanisms of exercise and DJC in treating diabetes.

Methods: Seventy-eight male Wistar rats were injected with low dose of streptozotocin and fed a high-fat diet to establish a diabetic model in rats. Then 60 diabetic rats were divided into diabetes group, exercise group, DJC group and exercise combined with DJC group. Another twelve rats were used as normal control. After eight months of treatment, the expression levels of phosphor-c-Jun N-terminal kinase (p-JNK), pancreatic and duodenal homeobox-1 (PDX-1), and insulin protein in pancreatic tissues from rats were detected by immunohistochemical method and Western blotting.

Results: In pancreatic tissues of diabetes group, the expression level of p-JNK protein was significantly higher than that in the normal group (P<0.01), and the expression levels of PDX-1 and insulin protein were significantly decreased (P<0.01). After administration of exercise and DJC, the expression level of p-JNK protein in pancreatic tissues of the diabetes group was decreased significantly, while the expression levels of PDX-1 and insulin protein were increased significantly (P<0.05 or P<0.01).

Conclusion: Exercise and DJC effectively protect islet β-cell function in diabetic rats, which might be due to a decreased JNK signaling pathway.

Key words: drugs,Chinese herbal, exercise, diabetes mellitus,experimental, insulin-secreting cells, JNK mitogen-activated protein kinases, rats

Figure 1

Immunohistochemical staining for detecting insulin in pancreatic islets of rats (light microscopy, ×400) A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise plus Danzhi Jiangtang Capsule group."

Figure 2

Immunohistochemical staining for detecting PDX-1 expression in pancreatic islets of rats (light microscopy, ×400) A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group. PDX-1: pancreatic and duodenal homeobox-1."

Table 1

Expressions of PDX-1 and insulin in pancreatic islets of rats (x±s)"

Group n PDX-1 Insulin
Normal 6 0.320±0.001 0.329±0.001
Untreated 6 0.143±0.010** 0.209±0.012**
DJC 6 0.216±0.025△△ 0.294±0.035△△
Exercise 6 0.242±0.022△△ 0.293±0.025△△
Exercise plus DJC 6 0.252±0.009△△ 0.316±0.014△△

Figure 3

Expression of PDX-1 in pancreatic islets of rats Data are expressed as x±s, n=4. **P<0.01, vs normal group; △P<0.05, △△P<0.01, vs untreated group. A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group.PDX-1: pancreatic and duodenal homeobox-1."

Figure 4

Expression of p-JNK in pancreatic islets of rats Data are expressed as x±s , n=4. **P<0.01, vs normal group; △P<0.05, △△P<0.01, vs untreated group. A: Normal group; B: Untreated group; C: Danzhi Jiangtang Capsule group; D: Exercise group; E: Exercise combined with Danzhi Jiangtang Capsule group. p-JNK: phospho-c-Jun N-terminal kinase."

[1] Abdelli S, Abderrahmani A, Hering BJ, Beckmann JS, Bonny C . The c-Jun N-terminal kinase JNK participates in cytokine- and isolation stress-induced rat pancreatic islet apoptosis. Diabetologia. 2007; 50(8):1660-1669.
doi: 10.1007/s00125-007-0704-2
[2] Kaneto H, Nakatani Y, Kawamori D, Miyatsuka T, Matsuoka TA, Matsuhisa M, Yamasaki Y . Role of oxidative stress, endoplasmic reticulum stress, and c^Jun N-terminal kinase in pancreatic beta-cell dysfunction and insulin resistance. Int[J] Biochem Cell Biol. 2006; 38(5-6) : 782-793.
doi: 10.1016/j.biocel.2006.01.004
[3] Armstrong MJ, Boulé NG , Sigal RJ- Exercise interventions and glycemic control in patients with diabetes. JAMA. 2011; 306(6):607.
doi: 10.1001/jama.2011.1103 pmid: 21828318
[4] Si XC, Shang WB, Bian HM, Cheng HB. A rat model of type 2 diabetes induced by streptozotocin plus high-calorie diet. Anhui Zhong Yi Lin Chuang Za Zhi. 2003; 15(5) : 383-395Chinese.
doi: 10.3969/j.issn.1672-7134.2003.05.012
司晓晨, 尚文斌, 卞慧敏, 程海波 . 链脲佐菌素加高脂膳食诱导2型糖尿病大鼠模型.安徽中医临床杂志. 2003; 15(5) : 383-395.
doi: 10.3969/j.issn.1672-7134.2003.05.012
[5] Ploug T, Stallknecht BM, Pedersen O, Kahn BB, Ohkuwa T, Vinten J, Galbo H . Effect of endurance training on glucose transport capacity and glucose transporter expression in rat skeletal muscle. Am[J] Physiol. 1990 ; 259(6 Pt 1):E778-E786.
[6] Chen MD . Effects of “glucotoxicity” “lipotoxicity” and “glucolipotoxicity” on islet β-cells. Zhonghua Nei Fen Mi Dai Xie Za Zhi. 2009; 25(1):5-8. Chinese with abstract inEnglish.
doi: 10.3760/cma.j.issn.1000-6699.2009.01.002
陈名道 . 胰岛P细胞的“糖毒性”､“脂毒性”与“糖脂毒性中华内分泌代谢杂志. 2009; 25(1):5-8.
doi: 10.3760/cma.j.issn.1000-6699.2009.01.002
[7] Liu HZ, Liu J, Bao TT, Fang ZH. The influence of Danzhi Jiangtang Capsule on function of B cells in patients with type 2 diabetes. Anhui Zhong Yi Xue Yuan Xue Bao. 2007; 26(4) : 5-7Chinese.
doi: 10.3969/j.issn.1000-2219.2007.04.003
刘怀珍, 刘剑, 鲍陶陶, 方朝晖 . 丹蛭降糖胶嚢对2型糖尿病患者B细胞功能的影响.安徽中医学院学报. 2007; 26(4) : 5-7.
doi: 10.3969/j.issn.1000-2219.2007.04.003
[8] Fang ZH, Ni YQ. Intervention effect of Danzhijiangtang capsule on vascular endothelium injury induced by oxidative stress in type two diabetes mellitus. Zhongguo Quan Ke Yi Xue. 2008; 11(10A):1745-1746. Chinese with abstract in English.
doi: 10.3969/j.issn.1007-9572.2008.19.008
方朝晖, 倪英群 . 丹蛭降糖胶嚢对2型糖尿病患者血管内皮氧化应激状态的影响. 中国全科医学. 2008; 11(10A):1745-1746.
doi: 10.3969/j.issn.1007-9572.2008.19.008
[9] Gu CY, Yuan L, Tang ZS, Liu Z, Zhu L. Effect of exercise on the morphology and function of pancreatic beta-cell in type 2 diabetes rats model. Zhonghua Wu Li YiXue Yu Kang Fu Za Zhi. 2005; 27(3):145-148. Chinese with abstract inEnglish.
doi: 10.3760/j:issn:0254-1424.2005.03.005
谷成英, 袁莉, 唐兆生, 刘赘, 祝炼 . 运动对2型糖尿病大鼠胰岛细胞形态与功能的影响.中华物理医学与康复杂志. 2005; 27(3):145-148.
doi: 10.3760/j:issn:0254-1424.2005.03.005
[10] Brownlee M . The pathobiology of diabetic complications : a unifying mechanism. Diabetes. 2005; 54(6):1615-1625.
doi: 10.2337/diabetes.54.6.1615
[11] Kawamori D, Kajimoto Y, Kaneto H, Umayahara Y, Fujitani Y, Miyatsuka T, Watada H, Leibiger IB, Yamasaki Y, Hori M . Oxidative stress induces nucleo-cytoplasmic translocation of pancreatic transcription factor PDX-1 through activation of c^Jun NH2-terminal kinase. Diabetes. 2003; 52(12) : 2896-2904.
doi: 10.2337/diabetes.52.12.2896
[12] Wu YJ, Fang ZH, Zheng SG, Wu YB, Lü MA, Wang Z, Fan AH. Danzhijiangtang Capsule and exercise on oxidative stress of pancreatic tissue and islet p-cell function in diabetic rats. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012; 32(11):1531-1534. Chinese with abstract in English.
doi: 10.7661/CJIM.2012.11.1531
吴元洁, 方朝晖, 郑书国, 吴元波, 吕明安, 王正, 范爱华 . 丹蛭降糖胶嚢联合运动对糖尿病大鼠胰腺氧化应激及胰岛功能的影响.中国中西医结合杂志. 2012; 32(11):1531-1534.
doi: 10.7661/CJIM.2012.11.1531
[13] Weston CR, Davis RJ . The JNK signal transduction pathway. Curr Opin Genet Dev. 2002; 12(1) : 14-21.
doi: 10.1016/S0959-437X(01)00258-1 pmid: 11790549
[14] Kaneto H, Kawamori D, Nakatani Y, Gorogawa S, Matsuoka TA . Oxidative stress and the JNK pathway as a potential therapeutic target for diabetes. Drug News Perspect. 2004; 17(7):447-453.
doi: 10.1358/dnp.2004.17.7.863704 pmid: 15514704
[15] Shiraki N, Lai CJ, Hishikari Y, Kume S . TGF-βsignaling potentiates differentiation of embryonic stem cells to Pdx-1 expressing endodermal cells. Genes cells. 2005; 10(6) : 503-516.
doi: 10.1111/gtc.2005.10.issue-6
[16] Stoffers DA, Kieffer TJ, Hussain MA, Drucker DJ, Bonner-Weir S, Habener JF, Egan JM . Insulinotropic glucagon-like peptide 1 agonists stimulate expression of homeodomain protein IDX-1 and increase islet size in mouse pancreas. Diabetes. 2000; 49(5) : 741-748.
doi: 10.2337/diabetes.49.5.741
[1] Álisson de Carvalho Gonçalves, Einy Jéssika Siqueira Moreira, Guilherme Vannucchi Portari. Benfotiamine supplementation prevents oxidative stress in anterior tibialis muscle and heart. Journal of Integrative Medicine, 2019, 17(6): 423-429.
[2] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[3] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[4] Hilal Yuksel, Yasemin Cayir, Zahide Kosan, Kenan Tastan. Effectiveness of breathing exercises during the second stage of labor on labor pain and duration: A randomized controlled trial. Journal of Integrative Medicine, 2017, 15(6): 456-461.
[5] Hidetaka Hamasaki. Exercise and gut microbiota: Clinical implications for the feasibility of Tai Chi. Journal of Integrative Medicine, 2017, 15(4): 270-281.
[6] Marjan Ahmad Shirvani, Narges Motahari-Tabari, Abbas Alipour. Use of ginger versus stretching exercises for the treatment of primary dysmenorrhea: A randomized controlled trial. Journal of Integrative Medicine, 2017, 15(4): 295-301.
[7] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[8] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[9] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[10] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[11] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[12] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[13] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[14] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[15] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[4] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[5] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[6] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[7] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[8] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[9] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[10] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3